Original title: HPLC-ED/UV for determination of vanillylmandelic acid in human urine after solid phase extraction
Authors: Němečková-Makrlíková, Anna ; Dejmková, H. ; Navrátil, Tomáš ; Barek, J. ; Vyskočil, V.
Document type: Papers
Conference/Event: International Students Conference “Modern Analytical Chemistry” /14./, Praha (CZ), 20180920
Year: 2018
Language: eng
Abstract: HPLC with electrochemical and spectrophotometric detection (ED/UV) after solid phase extraction (SPE) was used for determination of vanillylmandelic acid in human urine. HPLC-ED was performed at a glassy carbon electrode in a “wall-jet” arrangement in acetate-phosphate buffer at pH = 2.5 and gradient elution (increasing content of\nacetonitrile from 5 to 25% in 10 minutes) was used. Optimized parameters were following: flow rate of mobile phase 1 mL min−1, detection potential +1.1 V, detection wavelength 279 nm, injected volume 20 μL. Dependence of the peak current on the analyte concentration was linear in the concentration range from 10 to 150 μmol L−1, with obtained limits of detection 2.6 μmol L−1 (calculated from peak height) and 1.9 μmol L−1 (calculated from peak area) for HPLC-ED, and 11.0 μmol L−1 (calculated from peak height) and 9.8 μmol L−1 (calculated from peak area) for HPLC-UV.
Keywords: HPLC; solid phase extraction; tumor biomarker
Project no.: GA17-03868S (CEP)
Funding provider: GA ČR
Host item entry: Proceedings of the 14th International Students Conference “Modern Analytical Chemistry”, ISBN 978-80-7444-059-5

Institution: J. Heyrovsky Institute of Physical Chemistry AS ČR (web)
Document availability information: Fulltext is available on demand via the digital repository of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0290828

Permalink: http://www.nusl.cz/ntk/nusl-390729


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Research > Institutes ASCR > J. Heyrovsky Institute of Physical Chemistry
Conference materials > Papers
 Record created 2019-01-07, last modified 2019-03-28


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