Original title: First Report on Large Crabgrass Resistant to Atrazine in the Czech Republic
Translated title: První údaj o resistenci Digitaria sanguinalis vůči atrazinu v České republice
Authors: Salava, J. ; Chodová, D. ; Kočová, M. ; Krahulec, František
Document type: Papers
Conference/Event: Česká a slovenská konference o ochraně rostlin /17./, Praha (CZ), 2006-09-12 / 2006-09-14
Year: 2006
Language: eng
Abstract: [eng] [cze]
A survey on the occurrence of weeds surviving treatment with atrazine was carried out at the railways in the Czech Republic in 2000-05. Large crabgrass was widely distributed at the surveyed railway junctions. Seeds of large crabgrass were collected from plants growing in the surroundings of the railway station Prague-Bubny in 2003 and 2004. Resistance or susceptibility to atrazine was verified by the whole-plant response and chlorophyll fluorescence assays. A region of the psbA gene encoding D1 protein of Photosystem 2 was amplified by PCR and sequenced to determine the molecular basis for the atrazine resistance. It was found out that the resistance in the Prague-Bubny large crabgrass biotype was conferred by a glycine for serine substitution at residue 264 of the Dl protein of Photosystem 2. There was an excellent correspondence between the presence of the mutation and phenotypic resistance to atrazine of individual plants. V letech 2000-2005 byů sledován výskyt plevelů resistentních vůči atrazinovým herbicidům na železnicích České republiky. Digitaria sanguinalis je široce rozšířeným druhem na těchto železnicích a nádražích. Ze semen tohoto druhu sbíraných v železniční stanici Praha-Bubny v roce 2003-2004 byly vypěstovány resistentní rostliny, což bylo verifikováno jak odezvou celé rostliny, tak stanovením fluorescence chlorofylu. Úsek genu psbA kódujícího protein D1 fotosystému 2 byl amplifikován pomocí PCR a sekvencován k určení molekulárního podkladu resistence vůči atrazinu. Bylo zjištěno, že resistence tohoto biotypu rosičky vůči atrazinu je podmíněna nahrazením serinu glycinem v DI proteinu fotosystému 2.
Keywords: Digitaria sanguinalis; psbA gene; triazine resistance
Project no.: CEZ:AV0Z60050516 (CEP)
Host item entry: XVII. česká a slovenská konference o ochraně rostlin, ISBN 80-213-1516-4

Institution: Institute of Botany AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0153658

Permalink: http://www.nusl.cz/ntk/nusl-38088


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