Protilátky proti slinám flebotomů u domácích zvířat z endemických oblastí viscerální leishmaniózy

Košťálová, Tatiana

The main aim of this thesis was to test sera of domestic animals from endemic areas of visceral leishmaniasis in north and northwest Ethiopia for antibodies against presumed vector P. orientalis salivary glands and to clarify behavior of the sand fly and the role of domestic animals in transmission of visceral leishmaniasis. Specific IgG antibodies against P. orientalis saliva were tested in dogs, cattle, goats, sheep and donkeys. Above the cut-off value there was 76 % analyzed dogs, 15 % cattle, 26 % goats, 60 % sheep and 45 % donkeys. Dogs, goats and donkeys revealed significantly higher anti-P. orientalis antibodies compared to control animals. In the case of cattle, goats and donkeys cut-off levels were very high and is questionable if observed results are relevant. Canine sera were screened also for anti-P. orientalis IgG2 and 88% of Ethiopean sera revealed significantly higher IgG2 than control sera. The next aim was to detect if canine sera from Ethiopia with anti-P. orientalis antibodies react with other bloodsucking insect saliva by using immunoblotting method. In saliva of sand fly Sergentomyia schwetzi, which is abundant in Ethiopia, canine sera reacted mostly with 36, 37 and 51 kDa proteins. Mosquito Culex quinquefasciatus is cosmopolitan species and canine sera most intensely...

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