Original title:
Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
Authors:
Ledvina, Vojtěch ; Klepárník, Karel Document type: Papers Conference/Event: CECE 2016. International Interdisciplinary Meeting on Bioanalysis /13./, Brno (CZ), 20161017
Year:
2016
Language:
eng Abstract:
Caspases are proteases that play key role in the process of apoptosis, the programmed\ncell death. Among them, caspase-3 and -7 are main executioner caspases that cleave\nmany vital proteins during apoptosis and after their widespread activation, the process\ncannot be reversed. To analyze caspase-3/7 activation within single cells, a miniaturized\ndevice for parallel analysis of eight samples was developed. The assay is based on the\nmodified luciferin-firefly luciferase bioluminescence (BL) system. Individual\nsuspended cells were collected and transferred into detection microvials using a\nmicromanipulator. The bioluminescence was detected using a photon counting head\nwith cooled photcathode. The LOD suitable for detection of active caspase-3/7 in both\napoptotic and non-apoptotic cells was reached.
Keywords:
apoptotic cells; non-apoptotic cells; single-cell analysis Project no.: GA14-28254S (CEP) Funding provider: GA ČR Host item entry: CECE 2016. 13th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-4-4
Institution: Institute of Analytical Chemistry AS ČR
(web)
Document availability information: Fulltext is available in the digital repository of the Academy of Sciences. Original record: http://hdl.handle.net/11104/0265776