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Original title:
Fluorescent analysis of the differential protein expression in normozoospermic and asthenozoospermic sperm samples
Authors: Děd, Lukáš ; Čapková, Jana ; Kubátová, Alena ; Teplá, O. ; Pěknicová, Jana
Document type: Papers
Conference/Event: XXIst SYMPOSIUM OF BIOLOGY AND IMMUNOLOGY OF REPRODUCTION WITH INTERNATIONAL PARTICIPATION, Třešť (CZ), 2015-05-14 / 2015-05-16
Year: 2015
Language: eng
Abstract: Asthenozoospermia is one of the main seminal pathologies underlying male infertility. Previous proteomic studies have demonstrated the significant differences in the protein profiles between normozoospermic and asthenozoospermic sperm samples. Since these studies were primarily focused on the identification of differentially expressed proteins by mass spectrometry, we aimed to evaluate the ability of our diagnostic antibodies to detect the differential expression of selected protein markers by fluorescent microscopy and flow cytometry techniques. Therefore, we analyzed sperm samples from 30 men with normal and 30 men with astheno spermiograms, average by the panel of our diagnostic anti-human sperm (Hs) antibodies. Fluorescent microscopy and flow cytometry analysis revealed quantitative differences in the protein abundances between normo and astheno sperm samples, namely, in GAPDHs, evaluated with Hs-8 MoAb, VCP, evaluated with Hs-14 MoAb, and ATP synthase, evaluated with MoAb Hs-36. From the methodological point of view, we observed very high correlation between the data obtained by fluorescent microscopy and flow cytometry techniques and therefore both methods are useful for evaluation of protein differences associated with asthenozoospermia. From the clinical point of view, we observed the strong association of the low sperm motility in the sample with the expression of proteins, playing an important role in sperm energy metabolism (expected), but also with the expression of all tested intra-acrosomal proteins. These findings further demonstrate asthenozoospermia as a complex semen disorder frequently associated with other semen pathologies, which are not diagnosed by basic semen analysis, and the possibility to use monoclonal antibodies as a tool for diagnosis of protein associated sperm pathologies in the semen with the low sperm motility.
Keywords: asthenozoospermia; flow cytometry; monoclonal antibody
Project no.: GA P503/12/1834, GA 14-05547S, ED1.1.00/02.0109
Funding provider: GA ČR, GA ČR
Host item entry: Book of abstracts of XXIst Symposium of biology and immunology of reproduction
Institution: Institute of Biotechnology AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0262914
Permalink: http://www.nusl.cz/ntk/nusl-260982
The record appears in these collections:
Research > Institutes ASCR > Institute of Biotechnology
Conference materials > Papers
Authors: Děd, Lukáš ; Čapková, Jana ; Kubátová, Alena ; Teplá, O. ; Pěknicová, Jana
Document type: Papers
Conference/Event: XXIst SYMPOSIUM OF BIOLOGY AND IMMUNOLOGY OF REPRODUCTION WITH INTERNATIONAL PARTICIPATION, Třešť (CZ), 2015-05-14 / 2015-05-16
Year: 2015
Language: eng
Abstract: Asthenozoospermia is one of the main seminal pathologies underlying male infertility. Previous proteomic studies have demonstrated the significant differences in the protein profiles between normozoospermic and asthenozoospermic sperm samples. Since these studies were primarily focused on the identification of differentially expressed proteins by mass spectrometry, we aimed to evaluate the ability of our diagnostic antibodies to detect the differential expression of selected protein markers by fluorescent microscopy and flow cytometry techniques. Therefore, we analyzed sperm samples from 30 men with normal and 30 men with astheno spermiograms, average by the panel of our diagnostic anti-human sperm (Hs) antibodies. Fluorescent microscopy and flow cytometry analysis revealed quantitative differences in the protein abundances between normo and astheno sperm samples, namely, in GAPDHs, evaluated with Hs-8 MoAb, VCP, evaluated with Hs-14 MoAb, and ATP synthase, evaluated with MoAb Hs-36. From the methodological point of view, we observed very high correlation between the data obtained by fluorescent microscopy and flow cytometry techniques and therefore both methods are useful for evaluation of protein differences associated with asthenozoospermia. From the clinical point of view, we observed the strong association of the low sperm motility in the sample with the expression of proteins, playing an important role in sperm energy metabolism (expected), but also with the expression of all tested intra-acrosomal proteins. These findings further demonstrate asthenozoospermia as a complex semen disorder frequently associated with other semen pathologies, which are not diagnosed by basic semen analysis, and the possibility to use monoclonal antibodies as a tool for diagnosis of protein associated sperm pathologies in the semen with the low sperm motility.
Keywords: asthenozoospermia; flow cytometry; monoclonal antibody
Project no.: GA P503/12/1834, GA 14-05547S, ED1.1.00/02.0109
Funding provider: GA ČR, GA ČR
Host item entry: Book of abstracts of XXIst Symposium of biology and immunology of reproduction
Institution: Institute of Biotechnology AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0262914
Permalink: http://www.nusl.cz/ntk/nusl-260982
The record appears in these collections:
Research > Institutes ASCR > Institute of Biotechnology
Conference materials > Papers
Record created 2016-10-15, last modified 2021-11-24