Original title: Simple route of caspase-3 FRET sensor synthesis using “click chemistry”
Authors: Lišková, Marcela ; Křenková, Jana ; Klepárník, Karel ; Pazdera, P. ; Foret, František
Document type: Papers
Conference/Event: CECE 2015. International Interdisciplinary Meeting on Bioanalysis /12./, Brno (CZ), 2015-09-21 / 2015-09-23
Year: 2015
Language: eng
Abstract: Programmed cell death or apoptosis is regulated process of cell suicide. The central role in apoptosis play cysteine proteases called caspases. Caspases recognize tetra-peptide sequences Asp-Glu-Val-Asp (DEVD) on their substrates and hydrolyze peptide bonds after aspartic acid residues. Various techniques for the determination of caspase-3 are commercially available e.g. Enzyme Linked Immuno-Sorbent Assay (ELISA), Western blotting or flow cytometric analysis. The products of the cleavage can be detected by spectrophotometry, fluorimetry, chemiluminescence (CL) or ELISA. In this work, we suggested fluorescent sensor based on easily prepared Förster Resonance Energy Transfer (FRET). We use very simple chemistry called “click”. This type of chemistry takes advantages of quickness, simplicity and cheapness. “Click chemistry” is based on usage of various functional group and cross-linkers to combine individual molecules together.
Keywords: caspases; cell analysis; click chemistry
Project no.: GA14-28254S (CEP)
Funding provider: GA ČR
Host item entry: CECE 2015. 12th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-3-7

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available at external website.
External URL: http://www.ce-ce.org/CECE2015/CECE%202015%20proceedings_full.pdf
Original record: http://hdl.handle.net/11104/0250534

Permalink: http://www.nusl.cz/ntk/nusl-200768


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