Original title: Utilization of micropreparative fast focusing by a new wide pH range electrolyte system based on bidirectional isotachophoresis
Authors: Vykydalová, Marie ; Duša, Filip ; Horká, Marie ; Šlais, Karel
Document type: Papers
Conference/Event: CECE Junior 2013, Brno (CZ), 2013-11-12 / 2013-11-13
Year: 2013
Language: eng
Abstract: We suggest the possibility of practical utilization of a new electrolyte system for fast preparative focusing in wide pH range based on bidirectional isotachophoresis. The focusing occurs on nonwoven fabric strip positioned in an open horizontal V-shaped trough. It is based on bidirectional ITP with multiple counter ions and spacers created from commercially available simple buffers. Milk spiked with ampicillin was used as a sample and high performance liquid chromatography was as a second dimension for analysis of fast preparative focusing fractions. Speed, easy fraction handling, and possibility of pre-concentration of analytes from a raw sample are the benefits of this technique.
Keywords: bidirectional isotachophoresis; electrolyte system; preparative focusing
Project no.: VG20112015021 (CEP)
Funding provider: GA MV
Host item entry: Chemické Listy. Roč. 107, Issue s3., ISSN 0009-2770

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available on demand via the digital repository of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0229245

Permalink: http://www.nusl.cz/ntk/nusl-170566


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