Název: Adipokinetic hormone counteracts oxidative stress elicited in insects by hydrogen peroxide: in vivo and in vitro study
Překlad názvu: Adipokinetic hormone counteracts oxidative stress elicited in insects by hydrogen peroxide: in vivo and in vitro study
Autoři: BEDNÁŘOVÁ, Andrea
Typ dokumentu: Rigorózní práce
Rok: 2013
Jazyk: cze
Abstrakt: The involvement of members of Adipokinetic hormone (AKH) family in regulation of response to oxidative stress (OS) was investigated in the present study. OS was elicited in the insect body by exogenous application of hydrogen peroxide. In vivo experiments reveal that injection of hydrogen peroxide into the haemocoel of the firebug, Pyrrhocoris apterus, increases the level of AKH in the central nervous system (CNS) and in the haemolymph. The injection of hydrogen peroxide also increases the mortality of experimental bugs, whereas co-injection of hydrogen peroxide with Pyrap-AKH reduces mortality to control levels. Importantly, an increase in haemolymph protein carbonyl (an OS biomarker) levels is decreased to control levels when hydrogen peroxide is co-injected with Pyrap-AKH. Similar results were obtained using in vitro experiments. OS biomarkers such as malondialdehyde and protein carbonyls were significantly enhanced upon exposure of isolated CNS to hydrogen peroxide in vitro, whereas co-treatment of the CNS with hydrogen peroxide and Pyrap-AKH reduces their level significantly. Catalase activity was measured as well and the same pattern was observed. The results of the present study provide strong support on the feedback regulation between oxidative stressors and AKH action, and implicate AKH in counteracting OS.
Klíčová slova: Adipokinetic hormone; catalase; ELISA; hydrogen peroxide; malondialdehyde; oxidative stress; protein carbonyls; Adipokinetic hormone; catalase; ELISA; hydrogen peroxide; malondialdehyde; oxidative stress; protein carbonyls
Citace: BEDNÁŘOVÁ, Andrea. Adipokinetic hormone counteracts oxidative stress elicited in insects by hydrogen peroxide: in vivo and in vitro study. České Budějovice, 2013. rigorózní práce (RNDr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Instituce: Jihočeská univerzita v Českých Budějovicích (web)
Informace o dostupnosti dokumentu: Plný text je dostupný v digitálním repozitáři JČU.
Původní záznam: http://www.jcu.cz/vskp/35051

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-155492


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Školství > Veřejné vysoké školy > Jihočeská univerzita v Českých Budějovicích
Vysokoškolské kvalifikační práce > Rigorózní práce