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Utilisation of enzymatic labelling with 4-aminophtalimide and 4-hydroxybenzylideneimidazolinone fluorescent derivates for monitoring of DNA-protein interaction
Orság, Petr ; Pivoňková, Hana ; Riedl, Jan ; Hocek, Michal ; Fojta, Miroslav
The 5’-substituted deoxycytosine triphosphates with conjugated solvatochromic derivates of 4-aminophtalimide (API) and derivates of the green fluorescent protein, 4-hydroxybenzylideneimidazolinone (HBI) were synthetized and successfully tested for enzymatic incorporation using primer extension assay. Site specifically labelled oligonucleotide probes were prepared and tested for interaction with p53 and SSB proteins, displaying distinct DNA-binding properties. The incorporation of multiple fluorescent labels did not interfere with natural protein binding and protein interaction leaded in both cases the to the gradual ratiometric increase of the fluorescence intensity moreover accompanied with the changes of the fluorescence emission spectra profile. Neither effect was observed after incubation with BSA, non-DNA binding protein, confirming the specificity of the interaction. Modified nucleoside triphosphates with conjugated fluorescence labels derivates of API and HBI can be used as substrates for preparation of the specific oligonucleotide labelled probes and provide the novel tool for studying and monitoring the DNA-protein interaction.
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Řízení lidí v multikulturním prostředí
Moryc, Katarzyna Małgorzata ; Dvořáková, Zuzana (advisor) ; Vávra, Radovan (referee)
The main objective of the thesis was to define and analyze key aspects of managerial work in multicultural environment, in one of the Shared Services Center department, of multinational company based in Prague, Czech Republic. Further thesis aimed to define essential competencies of the successful manager and leader driving performance of multicultural department and provides recommendations to improve managerial performance in key aspects of manager s work such as communication, motivation, performance management and cross-cultural leadership. It is argued that presence of intercultural interactions between managers and their subordinates, impacts effectiveness of the managerial performance. Thesis consists of theoretical and practical part. First part of the thesis explores theoretical concepts regarding culture, communication, motivation and leadership with respect to multicultural environment specifics. In the second part, used research methods, conducted research analysis and outcomes are presented. Further in practical part recommendation towards analyzed aspects of managerial work that would lead to higher overall department performance are defined.
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Speculation on oil markets and its impact on commodity's price
Melcher, Ota ; Taušer, Josef (advisor) ; Baláž, Peter (referee) ; Müller, Štěpán (referee)
This study aims to analyse the precrisis period on the oil markets with a primary objective of assessing the role of speculation in the commodity's price development and its volatility. First it depicts the rapidly increasing speculative activity on the futures market together with the parallel oil price surge. The speculation is initially proxied by non-commercial traders' positions and subsequently quantified by Working's T-index. The paper then uses speculative traders' positions and both spot and futures prices to test for Granger causality within the framework of VAR models. For the sake of consistency it also evaluates causal links between speculation and inventories level. Further the study investigates the speculation impact on volatility of oil prices by employing various approaches in volatility quantification including GARCH models. Contrary to expectations we find that the speculatio's impact on both prices and their volatility is rather insignificant. In the last chapter we therefore seek for an explanation of the oil price developments by examining the market fundamentals. The interaction of supply and demand finally gives substantial evidence for understanding the price developments in the precrisis period.
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Analysing company's behaviour and communication on social networks
Němec, Milan ; Molnár, Zdeněk (advisor) ; Jelínek, Ivan (referee)
This thesis focuses on measuring company´s communication and behaviour on social networks. To capture the interaction between the company and its users the thesis uses web analytics tools. Therefore, the main objective of the thesis is analysis of these tools. To achieve this goal, the thesis characterizes social networks and their marketing opportunities for business promotion. Thanks to this, needs of using these tools is identified. The needs are: competitive analysis, optimizing communication, measuring achievement of objectives or detection competitive viral content. Research questions were determined and then analysis tools found answers to research questions. It was found that the tools provide various views of data and create a complete system for measuring and analysing behaviour and communication companies on social networks. The results were validated in a case study focused on the Facebook profile of the University of Economics in Prague. The study analysed the interaction profile of the school and its users and compared with others schools. In the results the analysis tools are validated and it is designed recommendation for improve engagement. This thesis revealed strengths and weaknesses of tools for measuring company´s communication and behaviour on social networks. It was found that these tools have wide range of use. They can be used for understanding the company's profile, its users, for purposes of competitive analysis, monitor corporate goals and optimize communications on social networks.
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Plasma spraying from liquids: plasma liquid interaction and coating build up
Tesař, Tomáš ; Mušálek, Radek ; Medřický, Jan ; Lukáč, František
Plasma spraying from liquid feedstocks is a rapidly developing field of thermal spraying since the coatings prepared from liquids exhibit some unique features, such as high hardness, thermal shock resistance or low thermal and electric conductivity. The key factor influencing the final coating character and properties is the input material which may be in the form of a suspension or a solution. Parameters of the selected suspension (solids concentration, viscosity, surface tension, chemical composition, etc.) or solution (concentration, etc.) determine its interaction with the plasma jet which strongly influences the coating buildup. This proceeding introduces the problematics of the interaction between the liquid feedstock material with the plasma jet and presents the way of evaluation of the coating buildup.
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Evaluation of the effectiveness of teaching vocational subjects depending on physical activity before schooling.
Janošíková, Martina ; Hanušová, Marie (advisor)
The period of transition to secondary school brings substantial changes in the requirements for study obligations, autonomy, communication, expression, or separated from their families in the form of staying at the dormitory. Students enrolled in sport centers, in addition to these changes, still struggling with the responsibilities associated with sport. Because they are students first through fourth grades of secondary schools and secondary vocational schools, an age when image self-I am, what I believe in, arises in interactions with the outside world and their own experience, plays a very important role to influence the environment, educators, teachers and trainers, with which students spend most of their time in practice. This study looks at students comprehensively in terms of personal development as a multifactorial biopsychosocial unit, in terms of cognitive and motivational processes, states and properties from the perspective of social psychology dealing with the interactions, relationships and communication, psychology of individuals within the social group and its importance for the development of the individual . It monitors conditions and educational outcomes in terms of educational psychology, dealing with forming properties, opinions, attitudes and value orientation from the perspective of educational psychology.
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Microbiota of the digestive tract of bees and related insect and the influencing facftors
Hroncová, Zuzana ; Havlík, Jaroslav (advisor) ; Kalous, Lukáš (referee)
This thesis consists of six research chapters, out of which, five have already been published in research journals, 1 is in the process of submission and the last chapter presents original unpublished confidential data in the process of manuscript preparation.
The research presented in this thesis concerns the complex mechanisms of bees and wasps immune system focused on microbiota as component of immunity. As shown in the introduction, highly social and managed species of bees like honey bees and bumble bees, play key roles in natural and agricultural ecosystems worldwide. Recent losses of bees have been attributed to pesticide exposure, poor nutrition, increased parasite loads and habitat degradation. Over the past several years, governments, beekeepers, and the general public worldwide have become concerned by increased losses of honey bee colonies, calling for more research on how to keep colonies healthy. Our main aim was to explore the complex mechanisms of bees and related species gut bacterial populations, their links to insect immunity and investigate the opportunities for an intervention. Part of our aim was to characterise the bumble bee and wasp gut microbiota using 16S RNA sequencing in a search for potentially novel bacterial species.
We have tried to explain how microbiome interacts with the host and showed that major members of these communities appear to benefit the host. The simple gut communities of social bees present ideal model systems to investigate the underlying evolutionary and genetic processes of such interactions. Information based on our results may help in the design of proper probiotic supplementation strategies with respect to physiological conditions in the honey bee gut.
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Properties of Aerosol, Produced by Laser Ablation of Standard Materials for ICP-MS Analysis.
Holá, M. ; Nováková, H. ; Ondráček, Jakub ; Vojtíšek, M. ; Kanický, V.
Laser ablation (LA), together with inductively coupled plasma mass spectrometry (ICP-MS) as a detection system, has become a routine method for the direct analysis of various solid samples. The product of laser ablation contains a mixture of vapour, droplets and solid particles. All components are finally transported to a plasma by a carrier gas as a dry aerosol including mainly agglomerates of primary nanoparticles. In general, characterisation of aerosols by their particle size distribution (PSD) represents indispensable tool for fundamental studies of the interaction of laser radiation with various materials. The particle size distribution of dry aerosol originating from laser ablation of standard material was monitored by two aerosol spectrometers – Fast Mobility Particle Sizer (EEPS) and Scanning Mobility Particle Sizer (SMPS) simultaneously with laser ablation - ICP-MS analysis.\n
Fulltext: content.csg -  PDF
Plný tet: SKMBT_C22016102412241 - PDF
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Study of dielectrical properties of organic material thin films
Pospíšil, Jan ; Boušek, Jaroslav (referee) ; Mikula, Milan (referee) ; Zmeškal, Oldřich (advisor)
The dissertation is focused on the study of electric and especially dielectric properties of thin film organic materials with their huge potential for optoelectronics and other industrial sectors. The theoretical part deals with the use of organic materials in organic photovoltaic cells, the methods of deposition techniques and characterization. The theoretical knowledge of the dielectric spectroscopy, including methods for determining the physical properties and evaluation of experimental data are also described. The experimental part is devoted to the study of small molecule organic solar cells with bulk heterojunction composed of electron donor molecule of DPP(TBFu)2 and electron acceptor fullerene derivatives, such as PC60BM, PC70BM and TC60BM. The experimental part is divided into two main parts. The first part deals with the study of processes occurring at the interface between the active layer and the contact; the second part is focused on transport processes inside the structure of photovoltaic cells and also contains a study of perovskite solar cells.
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