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Evaluation of the bred of cows in the system without market milk production at ecological and conventional farm
ROUŠALOVÁ, Miroslava
The aim of work was comparing breeding of suckler cows in agriculture companies with different methods of farming. Both farms are in the same climate conditions in submontaine region in Blanský Les. I included a herd of cows {--} bred Aberdeen Argus from the conventionally company and crossbreeds (the higher blood percentage of race Aberdeen Argus) are at the ecological farm. In both farms was checked same indices (reproduction inbreed, health problems cows and calves, management herd, sustenance, grazing system and economy of breed). Data was assessed behind period two years 2005 and 2006.
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The impact of South China Sea dispute on trade relations between China and Vietnam
Nguyen Hong, Viet ; Vlčková, Jana (advisor) ; Hasík, Gabriel (referee)
This bachelor thesis investigates the impact of South China Sea dispute on mutual relations between China and Socialist Republic of Vietnam. Thesis lays great emphasis on the analysis of mutual trading relations. The goal of this work is to find out if the dispute over territory has negative influence on mutual relations and to what extent. Therefore, thesis is divided into 3 main chapters. The introductory chapter describes position and development of each country. Second chapter uses findings from the introductory part for the analysis of political and trade relations of both countries. It focuses on the history of mutual relations and mainly it lays emphasis on current mutual relations. Last chapter of the thesis is dedicated to the issues regarding South China Sea. Motives and causes of this dispute are described in this chapter. It provides a view on the course of this territorial dispute, and also impacts on mutual relations are analyzed. The output of all three chapters helps to formulate following conclusion. Dispute over South China Sea has impact on mutual relations. Predominantly, it influences political relations between China and Vietnam, therefore, this dispute is often mentioned in media. However, according to available data this dispute has not had major effect on trading relations. Mutual trading between both countries went off in dispute period without any limitations and barriers.
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Fytochemical potencial of polyfenols contain in berries
KRUMPHANZLOVÁ, Iva
This thesis sums up findings on polyphenols and their positive influence on human health. In these fytochemicals, unlike in micronutrients, recommended daily amount cannot be estimated. Micronutrient deficiency in food can cause deficiency symptoms, while in polyphenol deficiency, the deficiency does not occur. When they are present in sufficient amount in food, it is considered functional food, which is important for civilization illnesses prevention. At the beginning of the theoretical part, the polyphenol classification from pharmacological and chemical point of view is represented. On the basis of this classification important role of antioxidants and flavonoids is described. Special attention is being paid to resveratrol, which is populardue to its well known "French paradox". The following part considers the polyphenol influence on human health. The way in which polyphenols protect our body from certain diseases is explained in detail. Most of the health benefits are supported by studies conducted on animals. These studies are also mentioned and described in this paper. In the theoretical part the process of absorption and the polyphenols safety for human body is also explained. Polyphenol sources are listed in the last subchapter. The work is mainly aimed at blueberry fruits (Vaccinium myrtillus) and vine grapes (Vitis vinifera). Other berries are briefly mentioned as well, such as cranberries, raspberries, blackberries and currant. The goal of this work is to create a diet that would provide a functional daily amount of polyphenols. This amount can be compared to 120 ml of native blueberries. Only products, consumable without cooking, are used in this diet. The second goal is to chart, which food products are adequate source of polyphenols. On the basis of these goals, research inquiries were defined. Research inquiry No. 1: What amount of polyphenols is contained in berries, especially blueberries. Research inquiry No. 2: Which food products (without cooking) are ideal to be included in diet, to provide adequate daily amount of polyphenols. To asses polyphenol content in blueberries, analysis of polyphenol content acquired through GEOMED program from Nemocnice České Budějovice a.s., department of clinical pharmacology, was used. Assessments were made using the HPLC method (high performance liquid chromatography) in cooperation with the biological centre AV ČR in České Budějovice. Thanks to this analysis, the first inquiry can be answered. An interesting phenomenon is discovered, that frozen blueberries have higher polyphenol content then cooled ones. Malvidin (malvidin-3-6"-acetoyl glycoside) was chosen as priority anthocyan since it is most represented in the blueberry. Remaining information about polyphenol content in certain food products were drawn on Joseph Maroon´s book, MD, FACS (2010). The practical part involves a diet for 14 days, which meets the given requirements. The results clearly show that it is possible to create a diet rich in polyphenols. A list of blueberry products accessible to everyone is included. Blueberry dairy products, jams, beverages, fruit salads and fruit pyre have been analysed. The list shows which product contains the most polyphenols. The required amount can be achieved by the proper combination of given products. The ideal one proved to be 200 ml of red wine and one blueberry dairy product (with the malvidin value higher than 64). Findings in this paper will serve to promote polyphenol awareness and itseffects. This diet can be used in nursing homes, where their positive influence on equilibrium can be applied. It can also be used in spas and other alimentation related facilities.
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Nutrition and other factors, which have influence on the formation and prevention of colorectal cancer
BENEŠOVÁ, Lucie
The full title of my bachelor thesis is Nutrition and Other Factors Influencing the Development and Prevention of Colorectal Cancer. In the theory section of the paper I have concentrated on clarifying the term "colorectal cancer", its epidemiology, and furthermore, on diet and other risk factors concerning the development and prevention of this disease. In this theory section I have included information about screening tests for occult bleeding. I have addressed the key objectives in the practical section of the thesis. I had set two key objectives. The first was to research how well respondents were informed concerning diets beneficial in the prevention of colorectal cancer, and the second objective concentrated on assessing the respondents' behaviour in relation to the information they had acquired. The research questions were formulated thus: "How well are people informed about diets aimed at preventing colorectal cancer?" and "Do people behave according to information thus gained?" The qualitative research method a semi-structured interview was used for the research section. Ten male respondents over the age of 45 took part in the interview. Five of these males were Dr Ilona Paseková's outpatients all with a genetic disposition to colorectal cancer, with most experiencing problems related to this disease. The other five respondents were without any predispositions. After receiving answers to the questions posed to the respondents, I wanted to compare both groups in order to ascertain whether those with a genetic predisposition showed a greater interest in this disease. Furthermore, I wanted to find out whether those respondents were actively seeking information concerning the prevention or treatment of colorectal and anal cancer. All those interviewed were very open and willing to answer questions. Collection of data was carried out from January 2014 to March 2014. The conclusions of this thesis show that public awareness of colorectal cancer and its prevention is inadequate. Public awareness of diets beneficial in the prevention of colorectal cancer is markedly poor, even among high-risk respondents. Having observed this lack of awareness, I proceeded to question respondents as to their dietary and lifestyle habits. Furthermore, the respondents were asked if they were aware that colorectal cancer screening was available in the Czech Republic. The results showed that some respondents, however unaware of diets beneficial in the prevention of colorectal cancer, follow a healthy diet, thus fulfilling dieticians' recommendations concerning the prevention of this disease. When asked about preventive foods, respondents most often mentioned brassicas broccoli and cabbage, etc. Conversely, the most often mentioned foods said to increase the risk of disease were smoked meat and red meat. One respondent mentioned the preventive affects of shark cartilage. I was very surprised by this assertion and subsequently searched the internet for information relating to shark cartilage. To my surprise, I found that the respondent was indeed not mistaken, and that statements to that effect can be found on websites promoting dietary supplements containing shark cartilage extract. According to these sources sharks do not suffer from cancer and therefore, their cartilage is closely studied and dietary supplements containing its extracts are produced. Furthermore, I intended to compare two groups of respondents those with genetic dispositions and those without. After carrying out the respective interviews, their comparison appeared to be futile. In both groups I identified respondents who followed a healthy diet and lifestyle as well as those who did not. I found that there is no relation between high-risk habits and predisposition to colorectal cancer. Whether a respondent with such a predisposition decided to follow a healthy diet or change his dietary habits and lifestyle or not was purely a question of his personal preferences.
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Freezing technology of bull sperm in relation to its survivability and fertilization ability
Doležalová, Martina ; Stádník, Luděk (advisor) ; Jiří, Jiří (referee)
The aim of optimalization the insemination doses production is to provide the highest fertilization ability of spermatozoa during the demanding proces of processing fresh semen and its subsequent cryopreservation. Temperature changes causes spermatozoa damage during the cooling and freezing. Spermatozoa is exposed to cold shock and many others limiting factors, which leads to cell death and therefore to decline of fertilization ability of thawed insemination doses. For increasing spermatozoa resistance, exactly the plasma membrane resistance against cold shock was fraction of egg yolk LDL cholesterol (low density lipoprotein) at various concentrations into the comercially produced diluents added. It is believed that LDL acts possitively to plasma membrane and helps to maintain the fertilization ability of spermatozoa after thawing. Following step in the proces of insemination doses production is slow cooling of diluted semen and equilibration, when the straws are store at cooling box for 30 minutes to 240 hours. This period is necessary to penetrate of certain diluent components into the spermatazoa also maintain the balance between their intracellular and extracellular concentration. Also important is subsequent freezing temperature gradient of insemination doses. The most suitable freezing method is based on computer controlled temperature decline in freezing chamber which allows the precise control of ice crystals formation that could tear and kill the cell.
During 2012 to 2016 was repeatedly collected semen from the group of breeding bulls (n = 27, Holstein and Czech Fleckvieh breed) at AI centre. Semen which fulfill the standard entrance conditions in first step was evenly into several parts divided. For dilution the three types of comercially diluents AndroMed, Bioxcell and Triladyl with and without LDL addition were used. Into the diluents AndroMed and Bioxcell the concentration of LDL 4 %, 6 % and 8% into the dilent Triladyl 6 %, 8 % and 10 % was added. Diluted semen was filled into the glass capillares with volume 0,1 ml and temperature +4 °C. Subsequently the sample was placed to cold bath (0°C) for 10 minutes. Then the volume of capillare with physiological solution (37 °C) was mixed and for next 120 minutes was incubate. The effect of cold shock to proportion of live spermatozoa was evaluated by using Eosin and Nigrosine staining technique during heat test of spermatozoa survivability after spermatozoa heating and after 120 minutes of incubation.
The more suitable semen diluents which provide the higher spermatozoa resistance against cold shock were AndroMed and Bioxcell. Together the possitive effect of LDL addition into the diluents to lower decrease of proportion of live spermatozoa during heat test was found (P<0.05). The most suitable LDL concentration which had a favorable influence at spermatozoa resistance against cold shock was 6 % in diluent Bioxcell. Values of the proportion of live sperm were higher at the beginning of the heat test (+1.31% to + 3.2%) and after 120 minute incubation (+5.82% to +8.41%) compared to other diluents with and without addition of LDL.
In the next step the process of equilibration was optimized, is an important part of insemination doses production. The effect of the length of equilibration for subsequent fertilization ability of spermatozoa was evaluated using spermatozoa motility based of CASA and proportion of live spermatozoa after thawing and during heat survival test lasting 120 minutes (37 ° C). Suitable semen was diluted by comercially used diluent AndroMed based on soya lecithin, filled into the straws (0.25 ml), cooled and equilibrated in cooling box for 30, 120 and 240 minutes and freezed in programmable freezing box applying four types of freezing curves differing in temperature rate decline. There was used standard and by producer recommended 3. phase freezing curve, then 2. phase freezing curve, and 3. phase freezing curve with slower as well as rapid decline of temperature rate in freezing chamber, compared with standard freezing curve. The highest spermatozoa motility was found using 240 minutes of equilibration by +2.72% and +4.58% compared to other lengths of equilibration (P <0.05 to 0.01). The highest proportion of live spermatozoa was found using 120 minutes of equilibration (+6.87 % and +8.68 %). The highest average spermatozoa motility during heat test after thawing was achieved by using 2. phase freezing curve (from +2.97% to +10.37%, P <0.05), also in the proportion of live spermatozoa (from + 4.37% to +8.82%, P <0.01). When evaluating interaction between the length of equilibration and freezing curve (standard 3. phase and 2 . phase freezing curve), the highest average spermatozoa motility and proportion of live spermatozoa using 240 minutes of equilibration by both freezing curves was reached, there was no statistically significant differences. As well as, in all evaluated parts of this study the individual differences between ejaculate of bulls and within semen from one bull (P <0.05) as secondary effect were found.
To maintain good fertilization ability of semen during cryopreservation is necessary to increase the spermatozoa resistance against cold shock using addition of correct concentration of LDL into the commercially used diluents AndroMed and Bioxcell. Subsequently the fertilization ability of insemination dose is influenced by cooling, the length of equilibration and freezing. The length of equilibration 120 minutes and more as well as gentle way of freezing according to freezing curve, which ensures a gradual decrease of temperature in freezing chamber provided the higher average spermatozoa motility and proportion of live spermatozoa.
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Non-invasive methods for sex and steroid homones determination in gibbons of the genus Nomascus
Bolechová, Petra ; Bartoš, Luděk (advisor) ; Pavel, Pavel (referee)
The gibbon primates of the family Nomascus are classified as critically endangered species, and, to date, basic understanding and information about their biology is missing. With regard to the status of these animals in the wild and captive populations in zoos, being familiar with their reproduction, may improve captive breeding programs. Data collection in this study was to be carried out by the practical use of non-invasive methods (polymerase chain reaction for DNA extraction and enzyme immunoassays), using faecal samples for analysis. The first method, polymerase chain reaction, was used for sex determination in juveniles, because of their coat colour and visual similarity of secondary sex characteristic in both sexes; it is not possible to determinate sex without handling the animal. Another main purpose of this study is to try and answer the hypothesis regarding the ovarian cycle of females, factors influencing their hormone concentration and also the onset of sexual maturity in females and the timing of their fur colour change. Hypotheses were checked by monitoring the concentration of progesterone and oestrogen faecal metabolites and by evaluation of the composition of breeding groups of gibbons with the ZOO influence. During a four year period (from 2010 till 2014), there were a total of 51 animals analysed from 16 different zoos with a faecal sample count totalling 1618 samples. The results confirmed the use of noninvasive methods for sex determination, and thus ensuring the maximum welfare standards. Endocrinological analysis confirmed the hypothesis of the influence of the environment (ZOO) to the hormone concentrations and female´s pregnancy effect in conjunction with her age on the final results of the male - father hormone concentrations. The initial information in this study is the confirmation of the onset of ovarian cycle in young females without connection to their fur colour change and a significant factor of a mother´s impact and her territoriality. This study is the first to present a long term monitoring of ovarian cycles in females and hormone concentrations of other individuals, both male and female, and from various age groups. The results allow us to understand the possible impact of zoo environments on the reproductive status of these gibbons and contribute to the general improvement of breeding management.
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A pair of phase-synchronized semiconductor lasers for length metrology
Hucl, Václav ; Holá, Miroslava ; Čížek, Martin ; Řeřucha, Šimon ; Pham, Minh Tuan ; Pravdová, Lenka ; Lazar, Josef ; Číp, Ondřej
Semiconductor lasers are increasingly widespread in areas that have been so far dominated by He-Ne lasers. One of such areas is the length metrology, where He-Ne laser operating at 633nm wavelength is still a traditional source of coherent light for laser interferometers distance measuring. The advantages of semiconductor lasers compared with He-Ne laser are greater optical power and a significantly wider range of wavelength tuning without mode jumps. Another advantage is the possibility of frequency modulation of wavelength of the laser diode by directly changing the injection current. The benefits predetermine these laser diodes for the realization of dual-frequency laser sources for heterodyne interferometry. In this case, using a phase-locked loop technique the optical frequency of a laser diode is locked to the optical frequency of another laser diode with a certain frequency offset in the MHz order. In our contribution we present utilization of DBR laser diodes in implementing a dual-frequency laser source for laser interferometry with heterodyne detection.\n
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Automatic system for gauge blocks calibration optimized for legal length metrology
Buchta, Zdeněk ; Šarbort, Martin ; Čížek, Martin ; Hucl, Václav ; Řeřucha, Šimon ; Pikálek, Tomáš ; Dvořáčková, Š. ; Dvořáček, F. ; Kůr, J. ; Konečný, P. ; Lazar, Josef ; Číp, Ondřej
This paper presents a contactless system for automatic and contactless gauge blocks calibration based on combination of laser interferometry and low-coherence interferometry. In the presented system, the contactless measurement of the absolute gauge block length is done as a single-step operation without any change in optical setup during the measurement. The optical setup is combined with compact gauge block changer with capacity 126 gauge blocks, which makes the resulting system fully automatic. \nThe paper also presents in detail a set of optimization steps which have been done in order to transform the original experimental setup into the automatic system which meets legal length metrology requirements. To prove the measurement traceability, we conducted a set of gauge block length measurement comparing data from the optimized system and the established reference system and TESA–UPC operated in Czech Metrology Institute laboratory.
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HUMAN ACTION RECOGNITION IN VIDEO
Řezníček, Ivo ; Baláž, Teodor (referee) ; Sojka, Eduard (referee) ; Zemčík, Pavel (advisor)
Tato disertační práce se zabývá vylepšením systémů pro rozpoznávání činností člověka. Současný stav vědění v této oblasti jest prezentován. Toto zahrnuje způsoby získávání digitálních obrazů a videí společně se způsoby reprezentace těchto entit za použití počítače. Dále jest prezentováno jak jsou použity extraktory příznakových vektorů a extraktory pros- torově-časových příznakových vektorů a způsoby přípravy těchto dat pro další zpracování. Příkladem následného zpracování jsou klasifikační metody. Pro zpracování se obecně obvykle používají části videa s proměnlivou délkou. Hlavní přínos této práce je vyřčená hypotéza o optimální délce analýzy video sekvence, kdy kvalita řešení je porovnatelná s řešením bez restrikce délky videosekvence. Algoritmus pro ověření této hypotézy jest navržen, implementován a otestován. Hypotéza byla experimentálně ověřena za použití tohoto algoritmu. Při hledání optimální délky bylo též dosaženo jistého zlepšení kvality klasifikace. Experimenty, výsledky a budoucí využití této práce jsou taktéž prezentovány.
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Text and Data Mining of Grey Literature for the Purpose of Scientific Research
Myška, Matěj
The paper explores the legal possibilities of users to text and data mine repositories of grey literature for scientific research without the consent of the GL repository operator and right holders of the documents contained therein. Firstly the scope of the respective copyright and sui generis database rights exceptions for scientifi c research is analyzed. Secondly the term “scientific research” and its meaning in the regulatory instruments is explored. Lastly the debated mandatory exception for TDM for scientific research is introduced.
Fulltext: idr-1037_3 -  PDF
Slides: idr-1037_1 - PDF; idr-1037_2 - PDF
Video: idr-1037_4 -  MP4
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