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Detection of biochemical substance using graphene sensor
Supalová, Linda ; Šimšíková, Michaela (referee) ; Bartošík, Miroslav (advisor)
Tato bakalářská práce studuje interakci biochemických látek s grafénem pomocí senzorů v uspořádání polem řízeného tranzistoru. Adsorbované atomy nebo molekuly mohou vyvolat dopování grafénové vrstvy, což může být experimentálně určeno pozorováním změny pozice Diracova bodu. Je studována závislost polohy Diracova bodu na přidané látce a stejně tak i časová odezva na přidání kapalného vzorku. Je pozorována citlivost na odlišné molekuly a jsou dikutovány důsledky výsledků pro adsorpci různých molekul.
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Calculation of Brown Coal Boiler for SCR Application
Mizerovský, Karel ; Bursa, Robert (referee) ; Skála, Zdeněk (advisor)
This Master´s thesis deals with the verification calculation of boiler which burns brown coal for the application of the selective catalytic reduction method. The thesis is divided into several parts. In the first of all is performed the calculation of the furnace chamber which involves stoichiometrics calculations and enthalpy calculations of air and flue gas. In the second part the heat balance of the boiler, the boiler losses and the thermal efficiency are determined. After all the heating surfaces are calculed for the verify the dimensions. In the conclusion of the thesis we looked for the temperature range which is used for the SCR application. Then the improvements for the appropriate temperature range are designed.
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DFT calculations of graphene regarding to biosenzoric applications
Špaček, Ondřej ; Friák, Martin (referee) ; Bartošík, Miroslav (advisor)
This diploma thesis is focused on calculation of both structure and electronic properties of the graphene after the adsorption of atomic and molecular oxygen and urea using the Density Functional Theory (DFT). The influence of van der Waals interactions on the structure and adsorption energy is studied, as well as influence of the thermal corrections, the charge density spatial distribution and the electronic doping of graphene after the adsorption of the adsorbant on the graphene.
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Utilization of light scattering techniques in study on protein denaturation
Köbölová, Klaudia ; Kalina, Michal (referee) ; Sedláček, Petr (advisor)
This bachelor thesis is focused on verifying the use of light scattering techniques in the study of protein denaturation. The theoretical part of the thesis describes proteins, their general properties, the process of denaturation and it provides an insight into light scattering. In the experimental part of the study I investigated the denaturation effects of four selected potential denaturing agents – urea, guanidine hydrochloride, sodium dodecyl sulfate, copper (II) sulfate – on two model enzymes: lipase and lysosyme. The denaturation effects of these denaturants were studied with the help of the dynamic light scattering method (DLS). In order to describe the change in particle size during denaturation, I used Z-Average, Volume mean and intensity distribution which were determined by this method.
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Technology of Selective Catalytic Reduction
Kudlík, Michael ; Janoušek, Michal (referee) ; Dundálek, Radim (advisor)
This bachelor thesis is focused on technology of selective catalytic reduction. The technology was created as a result of introduction of strict emission limits which manufacturers with this technology reach. First part of this thesis deal with the composition of the exhaust gases, where are dismembered the various elements, and then are given here the European emission limits.Then is thesis focused on ways of reducing emissions of diesel engines which describes the function of exhaust gas recirculation and a function of selective catalytic reduction. Then are described development systems selective catalytic reduction. The conclusion consists of the use and application of Automobile Manufacturers.
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Emission Levels for Tractor Engines
Vaško, Roman ; Beran, Martin (referee) ; Dundálek, Radim (advisor)
This bachelor’s thesis deals with the emission standards of tractor engines. There is a tightening of standards in recent year generally. The former part of the thesis contains description of specific pollutants that are exhaled into the air. The second part is focused on newly presented Tier 4i standards (Stage IV). The third part includes technologies, which provide engines to meet new regulations. The last chapters deal with an overview of the largest manufacturers of tractors, including Zetor, and their approach to emissions.
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Coated slow-release fertilizers based on PHB
Bobková, Magdalena ; Sedláček, Petr (referee) ; Kontárová, Soňa (advisor)
This bachelor thesis deals with the preparation, characterization and optimization of slow-release fertilizers coated with a solution of poly-3-hydroxybutyrate in dioxolane. First, manual formulations of fertilizers consisting of different ratios of urea and poly-3-hydroxybutyrate were prepared and coated, which were studied for the release of urea in the water environment. Most of the formulations released only about 50 % of the fertilizer after two months, so the potential for their use as slow-release fertilizers was proved. Furthermore, pellets with different ratios of urea and poly-3-hydroxybutyrate were also prepared using a quarter-operation pelletizer and coated in a coating drum. The pellets were studied in water and soil environments and certain mixtures in field tests. The delayed release of urea was also confirmed, and their ability to adequately nourish the plants was proved. The formulation consisting of 80 % urea and 20 % biodegradable poly-3-hydroxybutyrate in the matrix, in which 50 % of the fertilizer was released in water after 44 days, was chosen as the most promising one. In the soil environment, a slower nitrogen release was also confirmed for this formulation, and at the same time, a positive effect on the weight development of the above-ground parts of the plants and on the chlorophyll content in the leaves was observed in all formulations of the coated fertilizers.
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Kontrola výživy a metabolismu dojnic v chovu s automatizovaným systémem krmení a dojení
Záboj, Dominik
The aim of the thesis was to evaluate the effect of changing the technology of feed loading (conventional feed wagon loading vs. automated feed loading) on the content of milk components, especially on the concentration of urea as an indicator of feed balance over time. For the analysis of the milk constituent content in relation to ration balance, individual samples from the performance check, pool samples analysed by the dairy and herd averages from the Lely Astronaut A4 milking robot were used, which were collected from 1st January 2021 to 31st December 2022. The results of the study document that the change in feeding technology had neither a positive nor a negative effect on the milk constituent content. Statistical evaluation revealed only a significant difference in the number of somatic cells in milk (p < 0.01). Other evaluated milk composition parameters (protein, fat, lactose, urea) were not significantly affected by the change in feeding technology (p > 0.05). In the correlation test between pool samples and individual samples, statistically significant correlations were found for urea (r = 0.66), fat (r = 0.45) and somatic cell count (r = 0.63) at a significance level of p < 0.05. However, correlation analysis of milk testing results from robotic milking with data from pool samples and individual performance control samples yielded inconsistent data, with a positive correlation found only between the mean somatic cell count from the robotic milking parlor and individual KU samples (r = 0.22). In a set of examinations of individual milk samples from KU (n = 2112), correlation analysis was performed between all determined components. Statistically significant correlations (p < 0.05) were found between protein and fat (r = 0.82), lactose and fat (r = 0.68), somatic cell count and fat (r = 0.13), urea and fat (r = 0.45), lactose and protein (r = 0.80), somatic cell count and protein (r = 0.13), urea and protein (r = 0.47) and for urea and lactose (r = 0.45). It is clear from the results that the results of the analysis of milk from the robotic milking parlour differ from the standard values found in performance monitoring and in the examination of pool milk samples, which thus appear to be more suitable for monitoring nutrition and metabolism.
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Termín a způsob aplikace klasické a inhibované močoviny ve výživě kukuřice
Miloš, Lukáš
The aim of this bachelor thesis was to examine the effect of mineral fertilizers application on the grain yield of corn. The 4-year field experiment was conducted in years 2019-2022 in the form of small plot field experiment at the Field experimental station Žabčice. The primary objective of the experiment was to compare the method and term of application, as experimental fertilizers were chosen classic urea and its fully inhibited variant, thus urea with nitrification (IN) and urease (IU) inhibitor. The following treatments were included in the experiment: 1. Urea + IN + IU to the depot (autumn), 2. Urea to the depot (autumn), 3. Urea + IN + IU to the depot (spring), 4. Urea to the depot (spring), 5. Urea + IN + IU wide (spring), 6. Urea wide (spring).The work also focuses on average comparison of term (autumn, spring) and method of application (to the depot, wide), regardless of investigated fertilizer as well as to the average yield of grain after the application of the examined fertilizers (urea, urea + IN + IU). The average four-year results showed a difference of 4.3 % in grain yield after the application of urea fertilizer (8.38 t/ha), regardless of the term and method of application, compared to its inhibited variant (8.03 t/ha). The application of the examined fertilizers in autumn resulted in an increase in average grain yield by 5.8% compared to application in spring. The last factor investigated was the method of application, with maize fertilised widely yielding 10.3% higher compared to depot fertilisation.
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