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Biofilm formation in probiotic cultures and its application in pharmacy
Ryšávka, Petr ; Obruča, Stanislav (referee) ; Vorlová, Lenka (referee) ; Márová, Ivana (advisor)
The work was comprehensively focused on the development of adhesive forms of probiotics in the form of a biofilm on combined carriers with a prebiotic component. The second part dealed with the influence of food on the multiplication and survival of selected types of probiotic bacteria. Subsequently, the effect of individualized probiotic supplements on changes in the human intestinal microbiome was monitored. Suitable adherent probiotic strains for biofilm formation were selected and tested. Methods have been introduced and different variants of carriers for culturing and binding bacteria have been tested. In vitro experiments verified the stability of biofilm stucture and its resistance to low pH, bile and antibiotics in comparison with the planktonic cell form. The antimicrobial effect of probiotic strains in the form of a biofilm was studied. The cultivation of the multispecies biofilm on the combined carrier was optimized and the stability of the biofilm and the final viability of probiotic bacteria were confirmed. Furthermore, the influence of various foods and beverages on the viability of probiotic bacteria was evaluated with emphasis on the simulation of passage through the gastrointestinal tract. Both models, solutions with standardised concentrations of alcohol, sugar, salts, proteins or different pH and different types of real foods and beverages were tested. The effect of food and beverages was tested on monocultures of Lactobacillus acidophilus, Bifidobacterium breve and on probiotic capsules containing a mixed culture of probiotic microorganisms. The survival of probiotics in various food matrices in the simulated gastrointestinal tract was quantitatively different. We managed to define foods suitable for supporting the multiplication of probiotic bacteria. A separate part of the work was focused on the targeted modulation of the intestinal microbiome by individualized probiotics that were prepared on the basis of molecular biological analyzes of the intestinal microbiome aimed at detecting the percentage of lactobacilli, bifidobacteria and phylum Firmicutes and Bacteroidetes. Personalized probiotic supplementation confirmed the positive effect of this approach on microbiome changes, especially on the increase of the content of lactobacilli, bifidobacteria and the overall diversity of the microbiome.
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Wear regimes of aluminium alloys
Slivka, Václav ; Zapletal, Tomáš (referee) ; Šperka, Petr (advisor)
This thesis deals with the research of the wear regimes of the selected aluminium alloy in the conditions of dry friction. The aim of this work is the evaluation of the measure of an abrasive and adhesive wear of this alloy. This evaluation is made from the experiments which are made on the base of the knowledge of the research study. The dependence of the wear on the sliding speed and load, the friction on the sliding speed and load and the dependence of the normalised pressure on the normalised speed, so called the map of wear, are given on the base of the results of the measure.
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Surface modification of glass fibers for polymer composites
Knob, Antonín ; Marek,, Aleš (referee) ; Burša, Jiří (referee) ; Čech, Vladimír (advisor)
The doctoral thesis is aimed at preparation of glass fiber reinforced polymer composites with controlled interphase formed by plasma-polymerized tetravinylsilane and tetravinylsilane/oxygen thin films. The thin polymer films of specific physico-chemical properties and thickness were deposited to improve interfacial adhesion of glass fiber/polyester composites. The fiber surface modification was performed by using plasma enhanced chemical vapor deposition in low-temperature RF plasma operating in an various effective power range and different treatment time. Test results were examined in relation to the interlayer thickness and different treatment conditions. The prepared interlayers were analyzed to evaluate physico-chemical composition and properties (XPS, RBS, ERDA, FTIR and spectroscopic elipsometry). Selected mechanical properties were evaluated by AFM. Mechanical response of plasma interlayers was evaluated by short beam shear test and direct method of testing the interfacial shear strength using microindentation. The interphase shear failure was controlled by the shear strength at the interlayer/fiber interface as follows from experimental and model data.
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Surface modification of glass fibers using plasma nanotechnology
Sedlák, Filip ; Knob, Antonín (referee) ; Čech, Vladimír (advisor)
Diploma thesis is aimed at deposition of thin films on glass fibers using plasma-enhanced chemical vapor deposition from tetravinylsilane as a monomer. Such surface modified glass fibers were used as reinforcements for fabrication of polymer composites with unsaturated polyester resin as a matrix. Chemical and optical properties of prepared thin films were characterized using infared spectroscopy and spectroscopic ellipsometry. An influence of deposited thin films on the shear strength of composite material was observed using short-beam shear test.
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Mechanical response of long-fiber-reinforced polymer composite
Škriniarová, Nina ; Prokeš, Jan (referee) ; Čech, Vladimír (advisor)
This diploma thesis is focused on monitoring of mechanical response of long-fiber reinforced polymer composite. Main part of this thesis was preparation of long-fiber reinforced composite specimens, on which matrix was reinforced by commercially sized glass fibers. For comparison of properties were prepared specimens reinforced by unsized glass fibers. Apart from preparing specimens reinforced by long glass fibers were prepared specimens contains long carbon fibers. For evaluation of mechanical response of the prepared composite specimen were used flexural test and short beam shear test. Mechanical response was evaluated with universal testing machine ZWICK Z010 and data were processed in OriginPro 8 program. Thanks to evaluation of the mechanical response of the prepared specimens was assessed adhesion to fiber-matrix interface. By examining the mechanical response or adhesion can be assessed efficiency of commercial fiber surface treatment and so standardize measurement to compare other options of surface treatments.
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Top of rail lubricant development
Skurka, Šimon ; Polach, Pavel (referee) ; Kvarda, Daniel (advisor)
Friction modification within the wheel-rail interface is an important way of achieving ecologically friendly transportation of both persons and goods. This thesis aims to develop a new TOR lubricant, which will be able to maintain suitable frictional conditions while securing minimal adhesion required for traction. All measurements were carried out on tribometer MTM in the ball-on-disc configuration. In the first step, individual components were examined. More complex compositions were measured after that and the three best of them were compared with commercial TOR lubricants. The results show a good ability of developed compositions to maintain required adhesion, reduce wear, and all of them had resistivity against over-lubrication. Lastly, the process of lubricant verification before its application in real traffic was discussed.
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Effect of Interlayer Composition on Bond Strength between FRC Framework and Composite Veneer
Ibarra, Jonatanh José ; Pavelka,, Vladimír (referee) ; Poláček, Petr (advisor)
Hlavním cílem této práce je studium aktuálního problému adheze mezi kompozitními materiály, a určení vlivu složení mezivrstvy v pevnosti adhezivního spoje mezi vláknové (FRC) a částicové (PFC) kompozity, používané ve stomatologii. FRC tyčinky byly vytvořené na bázi komerční dimetakrylatové pryskyřice a skleněných S vláken. PFC byl vytvořen ze směsi pryskyřic (bis-GMA a PEGDMA), plněných drceným barnatým sklem. Celkově bylo připraveno a vyzkoušeno 84 vzorků. Vzorky byly rozdělené do pěti hlavních skupin. První skupina byla vytvořena ze série vzorků bez mezivrstvy. Zbylé čtyři skupiny byly rozděleny dle složení mezivrstvy (tloušťky a druhu pryskyřic). Částicové kompozity vzorků se lišily obsahem plniva (0, 10, 40 hm %). FRC tyčinky byly stejné pro všechny vzorky. Univerzální testovací přístroj ZWICK Z010 byl použit k zjištění smykové pevnosti adhezivního spoje všech vzorků. Rastrovací elektronový mikroskop byl použít k pozorování místa porušení. Ze získaných výsledků vyplívá, že s přidáváním mezivrstvy mezi vláknovým a částicovým narůstá smyková pevnost spoje. Důležitost těchto výsledků je způsobená tím, že v posledních letech použití těchto materiálů ve stomatologii narůstá a adheze zůstává jeden z hlavních problémů při klinické praxi.
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Design of Experimental Stand for the Study of Railway Vehicles Sanding
Galas, Radovan ; Hruška, Marek (referee) ; Šperka, Petr (advisor)
Presented master’s thesis describes the design and the implementation of an experimental device used for the study of tribological aspects of wheel-rail contact. In this work, the reader will first learn the fundamentals such as adhesion, slip, traction curve, etc. Subsequently, the work is describing devices that are used for the study of wheel-rail contact around the world. This section is followed by the author proposed solution. The result is a twin disc device in the scale of 1:3. The device allows determining traction characteristics for different environmental conditions (presence of water, sand and oil in contact for different temperatures) and various operating parameters (contact pressure and speed). A part of this work are also the validation experiments and complete design documentation.
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Effect of surface on bonded joint
Trhoň, Vojtěch ; Halamík, Tomáš (referee) ; Kubíček, Jaroslav (advisor)
This master’s thesis is divided into two parts. In the first part are these topics: bonding theory, treatment of bonding surface, types of adhesives, adhesives in the transportation industry and construction and stress of bonded joints. In the second part of this thesis is experimental evaluation of the effect of surface of material on strength of bonded joint.
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