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Conducting connections of ceramic with FR4 in 3D structure
Pelc, Miroslav ; Adámek, Martin (referee) ; Kosina, Petr (advisor)
This work deals with conductive connections ceramic substrate with FR4 into three – dimensional structure. High frequency amplifier was created on ceramic substrate as a concrete application, for check quality substrate on resulting behavior circuit. The supply voltage for this amplifier is provided by the DC voltage source developed for FR4. Further the thesis deals with quality verification of substances conductive connection and individual aspects, with affect resulting reliability of connection.
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The via interconnection in LTCC
Sedliak, Erik ; Klíma, Martin (referee) ; Kosina, Petr (advisor)
This thesis deals with manufacturing process of LTCC 3D structures. Emphasis is placed mainly on the formation of via interconnections. In the second section of thesis, different via interconnections were designed, created and tested. Aim of this thesis was to evalute impact of via properties on vias function.
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Web Server for Protein Structure Prediction
Votroubek, Lukáš ; Očenášek, Pavel (referee) ; Burgetová, Ivana (advisor)
This work deals with proteins, especially with their structure and kinds of tertiary, or 3D, structure prediction. Tertiary structure prediction is very important for function prediction of this vitally important substance. Bioinformatics do this prediction much more effective and faster, because classical methods of structure prediction directly from molecule are very expensive and slow. On the other hand they are much more exact. Objective of this thesis is to describe tertiary structure prediction methods, describe used tools and possibility of automatic communication with them. Next objective is describe implementation of server, that will serve to protein engineers for more effective finding of information about tertiary structure from more servers without requesting each of them separately. Results of testing will be described in this work too.
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Pressure sensor made 3D structure
Hanus, Martin ; Pekárek, Jan (referee) ; Kosina, Petr (advisor)
This thesis deals with the points at issue of a 3D structure pressure sensor. The pressure sensor converts pressure into another physical quantity which is easier measure and which is processed subsequently. Usually ways of pressure sensing is for example capacitive, strain-gauge and optical. The 3D structures are constructed through the use of a LTCC technology, which can be laminated to create multi-layer structures, compared to standard high-temperature ceramics. Passive circuit components can be applied onto particular ceramic layers using either thin-layer or thick-layer technology. Practice part of thesis deals with several ways of construction a pressure sensor. First of all capacitive, strain-gauge and optical sensor and possible problems with construction individual sensors above all problems with lamination, cavity or burning
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Wake Behind a Cylinder: An Overview of Spatio-Temporal Aspects
Uruba, Václav
A circular cylinder in crossflow is subjected to the overview study from the point of view spatial and temporal characteristics. It represents itself a typical engineering problem, appearing in practice frequently in various forms. In fluid mechanics, this case is considered to be a typical canonical case, with relatively simple and straightforward definition, but complex and dynamical flow topology. The typical dynamics is characterized by quasi-periodic behavior called von Kármán – Bénard vortex street with a typical frequency expressed in dimensionless number called Strouhal number. The wake flow structure is considered to be characterized by a single frequency and 2D topology, homogeneous along the cylinder axis, very often. The presented paper concentrates on differences between this commonly accepted model and physical reality. Both temporal and spatial aspects of the flow in the wake behind a cylinder are to be addressed. The turbulent subcritical wake characterized by Reynolds number about 5 thousand will be considered, as this situation is a typical case in mechanical engineering applications.
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Mass spectrometry in structural proteomics: study of viral protein complexes
RAVI, Anirudhan
This thesis investigated the avian reovirus sigma NS protein and its interaction with RNA using mass spectrometric methods. The sigma NS structure is still not known; thus, hydrogen-deuterium exchange and cross-linking coupled to mass spectrometry were employed to understand protein conformation. This research provides insights into protein-RNA interactions and the obtained data will be used in future work to predict protein structure.
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Structural characterisation of Prp45 and its interaction partners
Kulhánková, Lucie ; Novotný, Marian (advisor) ; Kostovčíková, Klára (referee)
Prp45 is a SNW protein that is part of a spliceosome and therefore participates in splicing of pre-mRNA into mRNA. In spliceosome Prp45 joins as part of NTC complex before splicing reactions. Prp45 has several known interactional partners (such as small subunit of U2AF, Cyp2, Prp5, Prp22, Clf1 and Cwc3). These proteins serve many functions in the spliceosome from stability to regulation of splicing reactions. Spliceosome consists of several dozens of proteins and several RNA and undergoes massive structural changes during executing its function. Until recently very little was known about the spliceosome structure; however, recent studies allow us to look at interaction network of this machine. In this work we focused on identification of both previously predicted and new interactional partners of Prp45. Using available spliceosome structures we aimed to describe these proteins structurally and functionally. Key words: splicing, Prp45, 3D struktura, spliceosome, binding partners
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Regulation of cathepsin K activity by reactive inhibitory molecules applicable in biomedicine
Benýšek, Jakub ; Mareš, Michael (advisor) ; Obšilová, Veronika (referee) ; Kutil, Zsófia (referee)
3 Abstract Human cathepsin K (KatK) is a lysosomal cysteine protease expressed predominantly in osteoclasts. It is the most effective enzyme for collagen breakdown and its physiological function lies in the degradation of the extracellular bone matrix. Increased enzymatic activity of KatK is associated with osteoporosis, rheumatoid arthritis, and osteoarthritis. This makes KatK a target for the treatment of pathologies, and chemotherapeutics based on protease inhibitors are being developed for its regulation. This work deals with reactive peptidomimetic and low molecular weight inhibitors of KatK namely thiadiazoles, vinyl ketones, and cyanohydrazides. It focuses mainly on the determination of the binding mode of selective inhibitors and characterization of their key interactions with the active site of KatK. Crystallographic structural analysis was combined with approaches of computational chemistry, enzymological analysis, and cell-based assays to elucidate the relationship between structure and biochemical activity of the investigated inhibitors. The obtained results provide important information for the design and optimization of new highly effective and selective KatK inhibitors as potential drugs and diagnostic probes. Keywords: cathepsin K, protease, inhibitor, 3D structure, osteoporosis
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On the 3D Dynamics of the Wake Behind a Circular Cylinder
Uruba, Václav ; Procházka, Pavel P. ; Skála, Vladislav
Flow in the wake of a circular cylinder is studied experimentally using time-resolved stereo PIV method. Special attention is paid to 3D topology of dynamical structures. While the distribution of statistic quantities along the cylinder is uniform, and i.e. 2D, the instantaneous flow structure is fully 3D. Within the velocity fluctuating flow field the structures containing streamwise vorticity and velocity components are dominant. The POD modes connected with von Kármán vortex street are identified.
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Structural characterisation of Prp45 and its interaction partners
Kulhánková, Lucie ; Novotný, Marian (advisor) ; Kostovčíková, Klára (referee)
Prp45 is a SNW protein that is part of a spliceosome and therefore participates in splicing of pre-mRNA into mRNA. In spliceosome Prp45 joins as part of NTC complex before splicing reactions. Prp45 has several known interactional partners (such as small subunit of U2AF, Cyp2, Prp5, Prp22, Clf1 and Cwc3). These proteins serve many functions in the spliceosome from stability to regulation of splicing reactions. Spliceosome consists of several dozens of proteins and several RNA and undergoes massive structural changes during executing its function. Until recently very little was known about the spliceosome structure; however, recent studies allow us to look at interaction network of this machine. In this work we focused on identification of both previously predicted and new interactional partners of Prp45. Using available spliceosome structures we aimed to describe these proteins structurally and functionally. Key words: splicing, Prp45, 3D struktura, spliceosome, binding partners
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