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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
The methodology of in vitro cultuvation of opium poppy and production of homozygous materials for breeding
 Klíma, Miroslav ; Vyvadilová, Miroslava ; Kučera, Vratislav
The methodology includes several options for preparing and maintaining initial donor F1 plants with respect to breeder’s possibilities. The next part presents the basic techniques for explant preparation, establishing of in vitro culture, regeneration of shoots and whole plants, transferring to non-sterile conditions, multiplying, identification and selection of diploid materials for further breeding. Attached there are specifications of cultivation media used. Important stages are documented with the help of figures.
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Method of testing of the seed traits and seedling development under abiotic stress conditions for selection of oilseed rape genetic resources
 Bláha, Ladislav ; Vyvadilová, Miroslava
The method is based on laboratory tests of speed of seed germination, emergence and seedling growth under different stress conditions simulated by subnormal water level, extreme high and low temperatures. It allows to eliminate already at the seed level the plant materials (initial breeding materials and cultivars) which do not tolerate extreme temperatures and temperature changes during germination, have a low water use efficiency and are intolerant to abiotic stresors. It was confirmed that these genotypes have also poor field emergence and initial growth of roots with implications for further vegetation period, mainly for over wintering and spring regeneration what has significant influence on the yield. The method represents the tool for screening of genetic resources with the resistance to the abiotic stresors and this technology process is acceptable also for other crops.
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