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Membrane endoglin and its role in pathogenesis of endothelial dysfunction in vitro
Vicen, Matej ; Nachtigal, Petr (advisor) ; Mladěnka, Přemysl (referee) ; Štěrba, Martin (referee)
Charles University, Faculty of Pharmacy in Hradec Kralove Department of Biological and Medical Sciences Candidate: M. Sc. Matej Vicen Supervisor: Prof. PharmDr. Petr Nachtigal, Ph.D. Title of Doctoral Thesis: Membrane endoglin and its role in pathogenesis of endothelial dysfunction in vitro Membrane endoglin (Eng) is a transmembrane glycoprotein that acts as a co-receptor in the transforming growth factor β (TGFβ) signalling cascade. Its expression and function have been extensively studied predominantly in relation to endothelial function under various pathological conditions, including hypercholesterolemia and atherogenesis. However, its role in the development of endothelial dysfunction remains controversial. Eng contributes to the formation of nitric oxide (NO) and is crucial in in vivo conditions for the development of the cardiovascular system and especially the heart. On the other hand, it may play an important role in inflammatory adhesion and transmigration of leukocytes to the endothelium, thus contributing to the development of endothelial dysfunction as the first stage of atherogenesis. Experiments on endothelial cells isolated from various blood vessels are used to study short-term effects of substances and drugs on the endothelium. One of the effects studied in endothelial cell...
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Effect of long-term exposure to soluble endoglin on the expression of adhesion molecules on endothelial cells
Tuschlová, Dominika ; Nejmanová, Iveta (advisor) ; Vicen, Matej (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Diploma Thesis: Effect of long-term exposure to soluble endoglin on the expression of adhesion molecules on endothelial cells Author of Diploma Thesis: Dominika Tuschlová Supervisor of Diploma Thesis: PharmDr. Iveta Najmanová, Ph.D. Background: The aim of this thesis was to find out the effect of long-term exposure to soluble endoglin (sEng) on the expression of adhesion molecules on endothelial cells (ICAM-1, VCAM-1, P-selectin). We have worked with a control and experimental group of mice on a standard diet, with different levels of sEng. Methods: Genetically modified female mice from the CBAxC57BL/6J strain with high production of human sEng were used for analysis. These mice formed an experimental group, while mice with a low level of sEng formed a control group. They were 12 months old females. We used biochemical analysis to determine the level of total cholesterol and triacylglycerols (TAG). Levels of sVCAM-1 markers in all mice studied were determined by ELISA. The expression of the adhesion molecules ICAM-1, VCAM-1 and P-selectin was monitored and evaluated by Western blotting, where the structural protein GAPDH was used as a control. Results: Biochemical analysis did not show a...
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Membrane endoglin and its role in pathogenesis of endothelial dysfunction in vitro
Vicen, Matej ; Nachtigal, Petr (advisor) ; Mladěnka, Přemysl (referee) ; Štěrba, Martin (referee)
Charles University, Faculty of Pharmacy in Hradec Kralove Department of Biological and Medical Sciences Candidate: M. Sc. Matej Vicen Supervisor: Prof. PharmDr. Petr Nachtigal, Ph.D. Title of Doctoral Thesis: Membrane endoglin and its role in pathogenesis of endothelial dysfunction in vitro Membrane endoglin (Eng) is a transmembrane glycoprotein that acts as a co-receptor in the transforming growth factor β (TGFβ) signalling cascade. Its expression and function have been extensively studied predominantly in relation to endothelial function under various pathological conditions, including hypercholesterolemia and atherogenesis. However, its role in the development of endothelial dysfunction remains controversial. Eng contributes to the formation of nitric oxide (NO) and is crucial in in vivo conditions for the development of the cardiovascular system and especially the heart. On the other hand, it may play an important role in inflammatory adhesion and transmigration of leukocytes to the endothelium, thus contributing to the development of endothelial dysfunction as the first stage of atherogenesis. Experiments on endothelial cells isolated from various blood vessels are used to study short-term effects of substances and drugs on the endothelium. One of the effects studied in endothelial cell...
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Effect of long-term exposure to soluble endoglin on the expression of adhesion molecules on endothelial cells
Tuschlová, Dominika ; Najmanová, Iveta (advisor) ; Vicen, Matej (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Diploma Thesis: Effect of long-term exposure to soluble endoglin on the expression of adhesion molecules on endothelial cells Author of Diploma Thesis: Dominika Tuschlová Supervisor of Diploma Thesis: PharmDr. Iveta Najmanová, Ph.D. Background: The aim of this thesis was to find out the effect of long-term exposure to soluble endoglin (sEng) on the expression of adhesion molecules on endothelial cells (ICAM-1, VCAM-1, P-selectin). We have worked with a control and experimental group of mice on a standard diet, with different levels of sEng. Methods: Genetically modified female mice from the CBAxC57BL/6J strain with high production of human sEng were used for analysis. These mice formed an experimental group, while mice with a low level of sEng formed a control group. They were 12 months old females. We used biochemical analysis to determine the level of total cholesterol and triacylglycerols (TAG). Levels of sVCAM-1 markers in all mice studied were determined by ELISA. The expression of the adhesion molecules ICAM-1, VCAM-1 and P-selectin was monitored and evaluated by Western blotting, where the structural protein GAPDH was used as a control. Results: Biochemical analysis did not show a...
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Cirkculating tumour cells and their role in breast cancer treatment
Kejzlarová, Eliška ; Svobodová, Zuzana (advisor) ; Vicen, Matej (referee)
The bachelor thesis summarizes the existing knowledge about circulating tumour cells (CTC) and their use in the diagnosis and treatment of breast cancer. First is presented a chapter about cancer in general. The following chapter deals with breast cancer, its etiology including genetic factors and other risk factors for its formation. Furthermore, there are types of breast cancer in women and men and the possibilities of their diagnosis and imaging methods. The next part is devoted to CTC, their definition and role in metastatic and primary breast cancer. Due to their low concentration in the blood of patients, special methods have been developed for their identification and detection. These methods are summarized in the following chapter. The number of CTCs in the blood of patients with breast cancer has a significant prognostic significance in the diagnosis and treatment by doctors, so it is important to monitor the current knowledge so that they can get into the common practice of cancer treatment as soon as possible. Key words: cancer, breast carcinoma, circulating tumour cells, diagnosis
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Microbiological load of raw materials (products) at downtimes in storage tanks and equipments
Kasalová, Lucie ; Vejsová, Marcela (advisor) ; Vicen, Matej (referee)
Lucie Kasalová Microbiological load of raw materials at downtimes in storage tanks and equipments Bachelor thesis Charles University, Faculty of Pharmacy in Hradec Králové Health bioanalytics Background: The aim of bachelor thesis is to find out whether the raw materials, used for food production, can be used for the recommended storage period but also after this period and to what extent they are affected by the increase of microbes. Further characterize the classical cultivation methods used in food microbiology and the process for the determination of microorganisms in food. In the experimental part, which deals with the testing of raw materials (fruit concentrates) stored in storage tanks, we try to find out to what extent they are subject to microbiological stress. For the comparison of the results of the experimental part, two sampling points will be selected - the first on the panel for the tank, the second on the mixing tank, where the raw material goes into the production part. Methods: The theoretical part is focused on description of cultivation methods used in microbiological laboratories for determination of individual microorganisms, especially determination of total number of microorganisms, determination of number of molds and yeasts etc. In the practical part, the differences in...
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