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One-trial association task as a basic biological model of episodic memory in rats
Radostová, Dominika ; Stuchlík, Aleš (advisor) ; Horáček, Jiří (referee) ; Telenský, Petr (referee)
One-trial association task as a basic biological model of episodic memory in rats Abstract A very important structure in the animal and human brain is the hippocampus. The study one of its many functions, episodic memory, is facing with the limited possibilities of non-invasive methods. In this regard, animal models are helpful. In order to study the individual components of such a complex ability in detail, a new behavioral task testing the association of temporally close but separate stimuli was developed. The test animals were exposed to a sequence of a sound conditioned stimulus and an unconditioned electric shock, between which there was a delay of 2 seconds. 59% of the rats successfully learned the association as well as the correct escape reaction to solve the task. Based on the results, the so-called One-Trial Trace Escape Reaction task could be a very valuable tool for a more detailed understanding of animals' incidental one-trial learning important for episodic memory. Another important phenomenon investigated in this work is the development of new neurons in the hippocampus in adulthood and its influence on learning and flexibility. After blocking the animals' neurogenesis with the temozolomide, the animals' ability to learn to avoid an invisible sector in the active avoidance task was tested....
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Animal models of obsessive-compulsive disorder
Radostová, Dominika ; Stuchlík, Aleš (advisor) ; Kopřivová, Jana (referee)
Obsessive compulsive disorder (OCD) is a widespread chronic psychiatric disorder, presenting itself with a wide range of symptoms. The heterogenity of OCD manifestations hinders the resarch and the interpretation of acquired results. Clinical studies are complemented by new insights collected from studies of animal models of OCD, which are nowdays integral to the resarch of OCD. During the course of the resarch many genetic, behavioral and pharmacologic animal models were developed. Number of works were not worked on further, however some models had been studied for a long time. Evaluation of individual models is dependant on many factors, and therefore the degree of validity and suitability for resarch differs between them. Powered by TCPDF (www.tcpdf.org)
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Vliv klomipraminu a risperidonu na učení a flexibilitu u animálního modelu obsedantně kompulzivní poruchy
Radostová, Dominika ; Stuchlík, Aleš (advisor) ; Kopřivová, Jana (referee)
Chronic sensitization of dopamine D2/D3 receptors by agonist quinpirole (QNP) induces compulsive checking behaviour in rats, which is considered an animal model of obsessive-compulsive disorder (OCD). Previous study revealed deficit in cognitive flexibility in QNP sensitized rats. This thesis focused on determining if this cognitive flexibility deficit is ameliorated by co-administration of clomipramine (CMI), risperidone (RIS) or combination of both (CMI+RIS) to QNP treatment. Aversively motivated active place avoidance task on a Carousel maze with reversal was used. The number of entrances into a to-be-avoided shock sector was evaluated as measure of performance. Six treatment groups were used: control group, QNP group, CMI group, QNP/CMI combination, QNP/RIS combination and QNP/CMI/RIS combination. Surprisingly, when compared alone, significantly worse acquisition was observed for QNP group compared to control group. However, similarly to previous study, QNP group had a worse performance in a first reversal session compared to control group. When all groups were compared, only QNP/CMI group had worse initial learning compared to control group. In reversal learning, only QNP treated group had a significantly more entrances than control group in first reversal session. Results suggest that co-treatment...
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Vliv klomipraminu a risperidonu na učení a flexibilitu u animálního modelu obsedantně kompulzivní poruchy
Radostová, Dominika ; Stuchlík, Aleš (advisor) ; Kopřivová, Jana (referee)
Chronic sensitization of dopamine D2/D3 receptors by agonist quinpirole (QNP) induces compulsive checking behaviour in rats, which is considered an animal model of obsessive-compulsive disorder (OCD). Previous study revealed deficit in cognitive flexibility in QNP sensitized rats. This thesis focused on determining if this cognitive flexibility deficit is ameliorated by co-administration of clomipramine (CMI), risperidone (RIS) or combination of both (CMI+RIS) to QNP treatment. Aversively motivated active place avoidance task on a Carousel maze with reversal was used. The number of entrances into a to-be-avoided shock sector was evaluated as measure of performance. Six treatment groups were used: control group, QNP group, CMI group, QNP/CMI combination, QNP/RIS combination and QNP/CMI/RIS combination. Surprisingly, when compared alone, significantly worse acquisition was observed for QNP group compared to control group. However, similarly to previous study, QNP group had a worse performance in a first reversal session compared to control group. When all groups were compared, only QNP/CMI group had worse initial learning compared to control group. In reversal learning, only QNP treated group had a significantly more entrances than control group in first reversal session. Results suggest that co-treatment...
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Animal models of obsessive-compulsive disorder
Radostová, Dominika ; Stuchlík, Aleš (advisor) ; Kopřivová, Jana (referee)
Obsessive compulsive disorder (OCD) is a widespread chronic psychiatric disorder, presenting itself with a wide range of symptoms. The heterogenity of OCD manifestations hinders the resarch and the interpretation of acquired results. Clinical studies are complemented by new insights collected from studies of animal models of OCD, which are nowdays integral to the resarch of OCD. During the course of the resarch many genetic, behavioral and pharmacologic animal models were developed. Number of works were not worked on further, however some models had been studied for a long time. Evaluation of individual models is dependant on many factors, and therefore the degree of validity and suitability for resarch differs between them. Powered by TCPDF (www.tcpdf.org)
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