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Relationship between tensile strength and hardness of aluminium alloys
Růžička, Martin ; Jetela, Václav (referee) ; Klement, Josef (advisor)
This thesis deals with relationship between tensile strength and hardness of aluminium alloys. The introduction provides an overview of aluminum aloys and their properties. In the following section there is an analysis of the processing of aluminum alloys. A large part is devoted to the course of precipitation hardening. The second part of the thesis contains a practical part, which describes the methodology of measuring hardness and tensile strength. The measurement process is described below. At the end, the measured data are processed into various graphs and their results are evaluated.
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Study of (bio)molecular interactions and enzymatic reactions by capillary electrophoretic methods
Růžička, Martin
Non-covalent interactions participate in majority of processes in living organisms. The strength of interaction between (bio)molecules can be characterized by binding constants of respective complexes, which can be determined by variety of physico-chemical methods. From these methods, capillary electrophoresis features several advantages: (1) interactions takes place in aqueous solutions between free molecules without necessity of their immobilization, (2) short analysis time, (3) small consumption of analyzed compounds, and (4) easy automation of analyses. Within this dissertation thesis, methods of partial-filling affinity capillary electrophoresis (PF-ACE) were developed for study of interactions between double-strand DNA oligonucleotides and well characterized intercalator ethidium bromide as a model compound. Subsequently, binding constants of oligophenylene derivatives complexes with DNA oligonucleotides were determined. The PF-ACE method was optimized for study of enantioselective interactions between helquats and selected chiral acidic aromatic analytes. Several compounds whose enantiomers were separated using helquats as chiral selectors were identified. Capillary electrophoresis was applied as a separation analytical method for monitoring of peptide substrate cleavage by rhomboid...
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Study of (bio)molecular interactions and enzymatic reactions by capillary electrophoretic methods
Růžička, Martin ; Hudeček, Jiří (advisor) ; Mikšík, Ivan (referee) ; Křížek, Tomáš (referee)
Non-covalent interactions participate in majority of processes in living organisms. The strength of interaction between (bio)molecules can be characterized by binding constants of respective complexes, which can be determined by variety of physico-chemical methods. From these methods, capillary electrophoresis features several advantages: (1) interactions takes place in aqueous solutions between free molecules without necessity of their immobilization, (2) short analysis time, (3) small consumption of analyzed compounds, and (4) easy automation of analyses. Within this dissertation thesis, methods of partial-filling affinity capillary electrophoresis (PF-ACE) were developed for study of interactions between double-strand DNA oligonucleotides and well characterized intercalator ethidium bromide as a model compound. Subsequently, binding constants of oligophenylene derivatives complexes with DNA oligonucleotides were determined. The PF-ACE method was optimized for study of enantioselective interactions between helquats and selected chiral acidic aromatic analytes. Several compounds whose enantiomers were separated using helquats as chiral selectors were identified. Capillary electrophoresis was applied as a separation analytical method for monitoring of peptide substrate cleavage by rhomboid...
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Design and evaluation of a user inteface for cinematic lighting
Růžička, Martin ; Křivánek, Jaroslav (advisor) ; Vorba, Jiří (referee)
This diploma thesis deals with global illumination and generally with the process of illumination of prepared scenes. A program for illumination management was written for this purpose. It can manage both direct and indirect illumination in interactive time. Simple and comfortable user interface allows for addition, deletion and change in light settings. Different types of both point and area lights are supported. In the course of all work, the program displays current illumination of the scene. With the help of this application, a series of different experiments will be carried out. We will explore the way users work during illumination, the way they perceive different properties of global illumination, various options of its control and its comparison with common direct illumination.
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Chicken antibodies as a tool of passive immunization against microbial diseases of respiratory tract
Růžička, Martin ; Hodek, Petr (advisor) ; Švédová, Martina (referee)
Pseudomonas aeruginosa and Burkholderia cepacia are opportunistic human pathogens. Hazards pose to immunocompromited patient groups, most of whom are patients suffering from cystic fibrosis, for which is the infection caused by Pseudomonas aeruginosa or Burkholderia cepacia, often lethal. Bacteria exhibit resistance to most antibiotics and the immunization of patients, paradoxically, worsens the patients' condition. Hen antibodies, unlike mammalian do not activate the complement cascade and do not cause the inflammatory response, therefore, seem to be a suitable tool to protect high risk populations as a means of passive immunization. In this work were prepared specific chicken antibodies against significant virulent factor - bacterial lectins PAIIL and BClA responsible for adhesion. Antibody specificity was demonstrated by ELISA and Western blot. Antibodies were affinity purified and cleaved to fragments. Pneumocytes type II from the lungs of patients with Cystic Fibrosis were isolated and cultured. Tests of the adhesion of bacteria cells were performed on them using ELISA. As an alternative model rat pneumocytes and tumor cell line A549 has been used. Prepared antibodies specifically detect bacterial lectins on the surface of bacteria. Antibody has been shown to reduce adhesion of bacteria to...
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Optimalization of somatic presumptions for artistic gymnastics
Růžička, Martin ; Krištofič, Jaroslav (advisor) ; Marek, Jaroslav (referee)
The Name of The Thesis: Optimalization of physical preconditions m Artistic Gymnastics The Objectvive of The Thesis: To analyse and evaluate data obtained by testing of individua! physical parametrs of men's and juniors' members of The Czech Republic national team relating to demands ofArtistic Gymnastics. Method: We use modification of Sheldon's Method by Carter and Heath to set up the somatotype. Individua! measured values of gymnasts and their diffusion are represented in one somatograph. Each measured item is compared with general population in graph. Values of juniors are compared with values gained from official materials of FIG. We use Matiegka's method to determine the percentage ofindividual physical tissues Results: Graphs containing results represent valuable information for coaches of men's artistic gymnastics in relation to the choice of talented individuals and to the optimalization ofphysical preconditions. Key words: Artistic Gymnastics, Somatotype, body constitution, rnen, juniors. 3
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Particle systems
Růžička, Martin ; Kmoch, Petr (referee) ; Pelikán, Josef (advisor)
The presented work deals with the issue of particles. It aims to offer a system for particle creation, simulation and visualization. The introductory part of the paper is devoted to design a physical model. The physical model particularly stresses a fast and at the same time plausible approximation of physical laws. Afterwards, it deals with methods of describing the particle behavior. In order to achieve the highest possible flexibility, a scripting interface is used. The conclusion then describes the visualization of the final particles itself. It is realized using the designed extension of the OpenGL graphic library. The results of the work can be seen in an enclosed application which demonstrates the possibilities of the designed particle systems.
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Fluid and structure interaction
Růžička, Martin
The subject of this thesis is the numerical simulation of the interaction of two-dimensional incompressible viscous flow and a vibrating airfoil. A solid airfoil with two degrees of freedom, which can rotate around the elastic axis and oscillate in the vertical direction, is considered. The numerical simulation consists of the finite element solution of the Navier-tokes equations coupled with the system of ordinary differential equations describing the airfoil otion. The time dependent computational domain and a moving grid are taken into account with the aid of he Arbitrary Lagrangian-Eulerian (ALE) formulation of the Navier-Stokes equations. High Reynolds umbers up to 106 require the application of a suitable stabilization of the finite element scretization. Numerical tests prove that used method is sufficiently accurate and robust.
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