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Evaluation of Flood Protection of Újezd u Brna
Lehotská, Lívia ; Jelínková, Iva (referee) ; Jandora, Jan (advisor)
The aims of the bachelor thesis is evaluation of flood protection of town Újezd u Brna and propose the increase levees of the Litava river or reduction levees under the city and realize the water from the river to inundation. The thesis will be transferred to the calculation using the HEC - RAS. The conclusion will be evaluated both proposals for protecting the city.
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Study of the action of ivermectin on purinergic P2X4 receptor
Jelínková, Irena ; Teisinger, Jan (advisor) ; Šťastný, František (referee) ; Krůšek, Jan (referee)
The P2X4 receptor is ATP-gated cation channel. It is the only mamrnalian purinergic receptor which is modulated by extracellularly applied ivennectin (IVM). rVM is an allosteric modulator that has several effects on receptor [unction: it increases sensitivity to agonists, potentiates maximum current amplitude and prolongs the deactivation kinetics of the channel after agonist washout. The aim of this study was to localize IVM binding site and using its positive allosteric effect to get new inforrnatioll about the structure and function of P2X. receptor. Initially we focused on identification of regions and residues responsible for IVM effect on channel function. We used several chimeras of P2X2 and P2X. receptors and P2X. receptors with single point mutatioll. Experiments with chimeric receptors revealed that extracellular sequence V49-V61 but not tbe sequ nce V64-Y315 is important for the effects af IVM on channel deactivation. Receptor-specific alanine mutations placed in transmembrane domains 029-V61 and N338-L358 showed the importance of residues W50, V61 and V357 for TVM effect Oll channel deactivation. We tested further the irnportance of aH residues in transmembrane domains. Cysteine scanning mutagenesis supported the relevance of previously identified W50 residue and showed the importance ofresidues...
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Solid-phase extraction and its miniaturization by method Lab-On-Valve for determination of pharmaceutical substances
Jelínková, Iva ; Chocholouš, Petr (advisor) ; Vlčková, Hana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical chemistry Candidate: Iva Jelínková Supervisor: PharmDr. Petr Chocholouš, Ph.D. Title of diploma thesis: Solid-phase extraction and its miniaturization by method Lab-On- Valve for determination of pharmaceutical substances This thesis deals with the development of the solid-phase extraction (SPE) method with the use of microextraction by packed sorbent (MEPS) for the determination of vitamins A, D, E and the possibility to automate this method. The determination was based on absorption of vitamins on microcolumn MEPS, interfering components were removed by washing solution (dilute acetic acid of pH 3) and extraction was performed by eluent solution (100% ACN). Detection was made by UV spectrophotometer at wave lengths of absorbing maximum for vitamin A - 325 nm, for vitamin D - 265 nm, for vitamin E - 295 nm. The method was optimized. There was developed a program, where concentrations of individual vitamins, dosing volumes, flow speed and composition solvents were tested. After the optimization method there was performed the extraction and the subsequent determination of blood plasma fortified with vitamins A, D, E by the sequential injection chromatography method. Recovery of the method for vitamin A was...
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The influence of syntactic structure (conjunctions) on spoken language (utterance section, intonation, pauses). Auditory analyses
Jelínková, Ivana ; Saicová Římalová, Lucie (advisor) ; Palková, Zdena (referee)
The presented thesis "The Influence of Syntactic Structure (Conjunctions) on Spoken Language (Tone Unit, Intonation, Pauses) - Auditory Analyses" explores the relations between conjunctions A (AND), ŽE (THAT), ABY (IN ORDER THAT), PROTOŽE (BECAUSE), ALE (BUT) in an exactly given syntactic structure and a system descritption of phonetic form of language - the tone unite, the intonation, and the pause. This research is based on the comparison of the public read and the public notread, but prepared utterances. At first we segment the chosen examples into the stress units; then we try to confirm the hypothesis that a conjuction begins a new tone unit; within the intonation, we monitor what is typical for the melodic line in the stress unit before the conjunction; the last part of our research is dedicated to pause and to their positions near the stress unit containing the conjunction. The research is based on the auditory analyses, therefore it does not use the instrumental analyses.
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The significance of extracellular DNA in osteoclastogenesis from peripheral blood precursors - in vitro study
Jelínková, Ivana ; Daňková, Pavlína (advisor) ; Korabečná, Marie (referee)
Introduction: Extracellular DNA (ecDNA) is a common component of blood plasma. Increased levels of ecDNA in plasma can be found in some autoimmune diseases like systemic lupus erythematosus (SLE), rheumatoid arthritis or celiac disease which are associated with inflammatory processes. These diseases are also associated with an increased risk of osteoporosis. Bone is a dynamic structure undergoing constant modelling caused by osteoblasts, osteocytes and osteoclasts. Shifting their equilibrium can lead to pathological conditions such as osteoporosis. In this thesis we focused on elucidating whether ecDNA, an inflammatory agent with proven immunoregulatory effects can alter differentiation potential of monocytes and alternatively lead to osteoclastogenesis via TLR9. Material and methods: We obtained monocytes from peripheral blood of healthy donors and cultivated them with four types of ODNs control (CO), stimulatory (ST), inhibitory (INH, telomeric (TLM) with phosphodiester (-pO) or phosphorothioate (-pS) backbone for two weeks to establish their effect on differentiation potential of monocytes into osteoclasts. Osteoclastogenesis was evaluated by number of yielded osteoclasts observed on a light microscope. To establish the effect of ODNs on osteoclast activity samples were analysed by qPCR for...
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Solid-phase extraction and its miniaturization by method Lab-On-Valve for determination of pharmaceutical substances
Jelínková, Iva ; Chocholouš, Petr (advisor) ; Vlčková, Hana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical chemistry Candidate: Iva Jelínková Supervisor: PharmDr. Petr Chocholouš, Ph.D. Title of diploma thesis: Solid-phase extraction and its miniaturization by method Lab-On- Valve for determination of pharmaceutical substances This thesis deals with the development of the solid-phase extraction (SPE) method with the use of microextraction by packed sorbent (MEPS) for the determination of vitamins A, D, E and the possibility to automate this method. The determination was based on absorption of vitamins on microcolumn MEPS, interfering components were removed by washing solution (dilute acetic acid of pH 3) and extraction was performed by eluent solution (100% ACN). Detection was made by UV spectrophotometer at wave lengths of absorbing maximum for vitamin A - 325 nm, for vitamin D - 265 nm, for vitamin E - 295 nm. The method was optimized. There was developed a program, where concentrations of individual vitamins, dosing volumes, flow speed and composition solvents were tested. After the optimization method there was performed the extraction and the subsequent determination of blood plasma fortified with vitamins A, D, E by the sequential injection chromatography method. Recovery of the method for vitamin A was...
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