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Chiral separations of borane cluster compounds.
Horáček, Ondřej ; Kučera, Radim (advisor) ; Šatínský, Dalibor (referee) ; Bednář, Petr (referee)
Univerzita Karlova, Farmaceutická fakulta v Hradci Králové Katedra farmaceutické chemie a farmaceutické analýzy Kandidát: Mgr. Ondřej Horáček Školitel: doc. PharmDr. Radim Kučera, Ph.D. Název: Chirální separace klastrových sloučenin boru Anorganické trojrozměrné klastrové sloučeniny boru se staly předmětem zájmu vědců zejména díky svým zajímavým vlastnostem, např. výjimečné tepelné, biologické a chemické stabilitě, neobvyklé třístředodové dvouelektronové vazbě a trojrozměrné aromaticitě. Podtřídou boranových klastrů jsou karborany, které mají v kleci inkorporován alespoň jeden atom uhlíku, čímž kombinují vlastnosti anorganických a organických látek v jedné molekule. Atom uhlíku umožňuje modifikaci klastru různými organickými substituenty, což vede k existenci nepřeberného množství derivátů. Z tisíců těchto sloučenin byly důkladně zkoumány deriváty aniontového bis(dikarbolidu) kobaltu ([Co(C2B9H11)2]- ), a to zejména ve farmaceutické chemii, chemii materiálů, elektrochemii a analytické chemii. Zvýšený zájem o [Co(C2B9H11)2]- je obvykle vysvětlován jeho rozpustností ve vodě, chemickou a biologickou stabilitou, nízkou toxicitou, skrytým amfifilním charakterem a podobností s chemií ferrocenu. Mnoho derivátů [Co(C2B9H11)2]- uvedených v literatuře je chirálních. Navzdory jejich potenciálnímu využití ve...
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Chiral separations of borane cluster compounds.
Horáček, Ondřej ; Kučera, Radim (advisor) ; Šatínský, Dalibor (referee) ; Bednář, Petr (referee)
Univerzita Karlova, Farmaceutická fakulta v Hradci Králové Katedra farmaceutické chemie a farmaceutické analýzy Kandidát: Mgr. Ondřej Horáček Školitel: doc. PharmDr. Radim Kučera, Ph.D. Název: Chirální separace klastrových sloučenin boru Anorganické trojrozměrné klastrové sloučeniny boru se staly předmětem zájmu vědců zejména díky svým zajímavým vlastnostem, např. výjimečné tepelné, biologické a chemické stabilitě, neobvyklé třístředodové dvouelektronové vazbě a trojrozměrné aromaticitě. Podtřídou boranových klastrů jsou karborany, které mají v kleci inkorporován alespoň jeden atom uhlíku, čímž kombinují vlastnosti anorganických a organických látek v jedné molekule. Atom uhlíku umožňuje modifikaci klastru různými organickými substituenty, což vede k existenci nepřeberného množství derivátů. Z tisíců těchto sloučenin byly důkladně zkoumány deriváty aniontového bis(dikarbolidu) kobaltu ([Co(C2B9H11)2]- ), a to zejména ve farmaceutické chemii, chemii materiálů, elektrochemii a analytické chemii. Zvýšený zájem o [Co(C2B9H11)2]- je obvykle vysvětlován jeho rozpustností ve vodě, chemickou a biologickou stabilitou, nízkou toxicitou, skrytým amfifilním charakterem a podobností s chemií ferrocenu. Mnoho derivátů [Co(C2B9H11)2]- uvedených v literatuře je chirálních. Navzdory jejich potenciálnímu využití ve...
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Physiotherapy procedures in patients after disc surgery in lumbar spine
Kozohorská, Václava ; Babková, Lenka (advisor) ; Horáček, Ondřej (referee)
This thesis focuses on problems of physiotherapy procedures after a surgery of intervertebral disc in the meaning of intensity, timing and therapeutic procedures. In the general part it gives comprehensive overview about functional anatomy, kinesiologi, biomechanics of the spine and about the function of intervertebral disk. It also deals with methods of investigation and surgical interventions. In the special part, the thesis focuses on the most appropriate choice of timing, intesity and mainly on special methods which are the most commonly used in patients after surgery of intervertebral disc. It gives the basic charakteristics of each special methods in relation to patients after surgery of intervertebral disc. The practical part of this thesis includes patient case report after surgery of intervertebral disc which describes the examination before neurosurgical intervention, surgery, hospitalization and outpatient rehabilitation.
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Calculations of parameters of biologically active compounds by the quantum mechanical methods.
Horáček, Ondřej ; Palát, Karel (advisor) ; Krátký, Martin (referee)
8 ABSTRACT The aim of this work is to calculate the parameters of substituted bases and chosen phenylguanidinium cations, earlier synthesized at the Department of inorganic and organic chemistry on the Faculty of Pharmacy CU in Hradec Králové, using quantum chemical methods. I solved chosen problem by using computer programs Gaussian 03W and Hyperchem 8.0.10. I used program Gaussian to optimize the molecules and calculate the energy of the HOMO and LUMO, total energy and the atomic partial charge using the Mulliken population analysis. I used programe HyperChem to calculate the Van der Waals molecular volume; Van der Waals molecular surface; logP; the molar refraction; solvent-accessible surface of the probe size 1.0 Å; 1.2 Å; 1.4 Å; 1.6 Å and the atomic partial charge using methods PEOE. All parameters of selected substituted phenylguanidines above have been successfully calculated, and listed in the tables in the chapter called results. The calculated results were discussed in chapter called discussion. The calculated parameters will be used for further correlations and QSAR studies that will help to understand the biological activity of substituted phenylguanidines and hence their effect on the human organism.
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Examination of vegetative system in hereditary neuropathy
Jílková, Daniela ; Horáček, Ondřej (advisor) ; Kobesová, Alena (referee)
Aim: The aim of this thesis "Examination of vegetative system in hereditary neuropathy" is to consider the level of physical fitness, physical actvity level and quality of autonomic function in patients with hereditary neuropathy Charcot Marie Tooth and an assessment of interactions of these parameters, especially with regard to possible influence by the presence of autonomic neuropathy and with regard to neurological symptoms. Background: Although vegetative neuropathy was mentioned in the first publication about CMT, it is not widely discussed topic as part of it. Methods: Seventeen probands underwent spiroergometry, heart rate variability test, six minute walk test, rating by the CMT Neuropathy Score and Overall neuropathy disability scale and completing the IPAQ questionnaire. Results: We found symptoms of autonomic neuropathy in group of patients with CMT, especially high-frequency part of heart rate variability spectrum has a particularly significant decrease. Patients with autonomic neuropathy have shown a low fitness and functional capacity, tendency to obesity and hypertension. The observed correlation of autonomic neuropathy and neurological impairment was very weak. Conclusions: We find a neuropathy of the autonomic nervous system in patiens with hereditary motor and sensitive neuropathy...
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Gait disorders in hereditary neuropathy
Linhartová, Petra ; Horáček, Ondřej (advisor) ; Truc, Michal (referee)
Hereditary motor sensory neuropathy (CMT) exhibits characteristic symptoms, i.e. specific gait impairment. Little detailed information has been written on the gait analysis of this disease. The aim of this work was to collect information about gait disorders in patients with CMT, to perform a threedimensional gait analysis and prove the effectiveness of rehabilitation. There was a detailed kinesiological analysis, muscle strength test and Coda Motion System gait analysis done on one patient. The patient has visited rehabilitation for 12 weeks and after, the whole spectrum of examinations were repeated. Within the framework of the gate analysis there were the joint ranges in flexion-extension of the hip, knee and ankle evaluated and compared with the normal gait. After undergoing rehabilitation there was subjective improvement in the quality of walking and objective improvement compared to the initial examination's findings. This acknowledges the results of the kinesiological analysis, muscle strength test and Coda Motion System gait analysis. Results of this work provide an overview of the patterns of gait disorders in CMT and support the importance and effectiveness of rehabilitation in improving gait quality in CMT patients. Powered by TCPDF (www.tcpdf.org)
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Surgical treatment of Brachial plexus birth palsy and possibilities of Physiotherapy
Šolcová, Monika ; Zounková, Irena (advisor) ; Horáček, Ondřej (referee)
Obstetrical palsy of upper limb results from a perinatal lesion of brachial plexus nervous bunch. The incidence of this disease ranges from 0.42 to 5.1 / 1,000 live births. If the palsy isn't diagnosed early and treated adequately, child will be threatened by major consequences later. This work provides the reader with the current view on the surgical treatment of patients with brachial plexus birth palsy. It gives a comprehensive overview on early neurosurgical intervention and secondary orthopaedic reconstruction. It submits opinions on indicating criteria. It describes the main charakteristic and results of operations. It refers to the fact that the choice of a surgical procedure can be essential in therapy. The aim of this work is also to characterize the fundamental aspects of postoperative physiotherapy, which simultaneously goes along with the process of intervention therapy. The practical part of this work includes a case of a patient after a neurosurgical performance. It describes the preoperative examinations, the performed surgery and the implementation of postoperative rehabilitation.
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Achiral and chiral high performance liquid chromatography of drugs and their metabolites III.
Horáček, Ondřej ; Nobilis, Milan (advisor) ; Mokrý, Milan (referee)
Nabumetone is non-steroidal anti-inflammatory prodrug that is converted into (6-methoxynaphthalen-2-yl)acetic acid in the liver. The differences of in vitro nabumetone biotransformation (rat vs human liver cytosolic and microsomal fractions) were studied in previous communication. Study of stereospecificity of enzymes and the calculation of enantiomeric ratios of the biotransformed chiral metabolites is an equally important characteristic of enzymes such as the Michaelis constant. Calculation of enantiomeric ratios of chiral metabolites biotransformed from prochiral drugs in vitro is used to identify new enzymes responsible for the biotransformation of a given prochiral drug. This work deals with the optimization of separation and detection conditions for achiral and chiral LLE-HPLC-PDA analysis of nabumetone and its six metabolites in extracts from various biomatrixes (human liver microsomal and cytosolic fractions and minipig blood plasma). Differences in biotransformation of nabumetone and racemic mixture of 3-HO-nabumetone between man and woman were studied in vitro in extracts from liver cytosolic and microsomal fractions, using chiral LLE-HPLC-PDA analysis. Enantiomeric ratios of all chiral metabolites of nabumetone were calculated. Pharmacokinetics of 6-MNA in minipig blood plasma after...
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