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Robot Localization Using Camera
Heřman, Petr ; Španěl, Michal (referee) ; Beran, Vítězslav (advisor)
The objective of this work is to design a simple localization method and its implementation in robot operating system ROS. This method uses a monocular camera as the only sensor and estimates the position in a known map. In experiments with prototypes are tested key points of type SURF, SIFT and ORB.
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Marketing Communication Mix
Norek, Milan ; Heřman, Pavel (referee) ; Kaňovská, Lucie (advisor)
Bachelor thesis is concerned with analysis of the current marketing situation of the company and the proposals in the area of comunication mix. The first part consists of basic terms and marketing theory. Analytical part focuses on introduction of the company and analysis of the current state. Solutions are proposed and evaluated in the last part.
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Robot Controlled by PIC Microprocessor Unit
Heřman, Petr ; Luža, Radim (referee) ; Rozman, Jaroslav (advisor)
This thesis describes design of a cheap robot. It includes implementation of firmware of low level control unit based on microcontroller PIC. The firmware drives motors, gains sensors data and communicates with the high level control unit. Furthermore the thesis presents realisation of connection to the robotic operation system ROS and its standard structures allowing usage of existing packages for the robot teleoperation and displaying sensor data on the remote computer. The thesis finally reports experiments with the robot. The constructed prototype is the model of the robotic lawn mower, however the whole solution has universal usage.
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Engines for Le Mans Series
Herman, Pavel ; Blaťák, Ondřej (referee) ; Svída, David (advisor)
This bachelor´s thesis deals with the overview of development the drive units, which are used by racing cars of Le Mans endurance series, mainly the race 24 hours of Le Mans. In the first part is outlined the general characteristic the series and the race 24 hours of Le Mans itself. There are briefly described the instructions and the most important technological limitations, observed by designers. The second part contains an owerview of racing cars engines, which are interesting from the historical point of view either by their construction or by achieved success. The last part is devoted to the constructional comparison of drive units of present participants the Le Mans series, their description and the important technical specifications.
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Proposal for Using Virtual Reality Technology in Marketing of a Company
Šmerda, David ; Herman, Pavel (referee) ; Kaňovská, Lucie (advisor)
This Bachelor Thesis describes the current advent of virtual reality (VR) technology and possible use of this technology in marketing of the company. I will apply this technology to PTÁČEK - velkoobchod a. s. In the first part I will focus on the theory of marketing, marketing communication, marketing trends including virtual reality and psychological aspects in connection with VR. In the second part I will introduce the company PTÁČEK - velkoobchod a. s. And using selected analyzes I will analyze its marketing environment, which I will summarize in the resulting SWOT analysis. The third part contains a proposal to improve the company's marketing through VR technology, it‘s implementation and solution in practice.
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The influence of disintegration of the input material for the production of methane by anaerobic fermentation
Herman, Pavel ; Frýba, Lukáš (referee) ; Houdková, Lucie (advisor)
This master‘s thesis deals with the influence of disintegration of brewer's grains for the production of biogas by anaerobic fermentation. The first part introduces us with the issue of biodegradable wastes. There are given their characteristics, ways of handling with them and legislative restrictions associated with them. In other sections there is a detailed description of anaerobic fermentation itself, including characteristics of biogas. Then there is issue of disintegration during fermentation including a list and characteristic of some disintegration methods. The main part of the thesis consists of description of the experiments on which it is observed increment of biogas based on the disintegration of brewer's grains and the evaluation of the individual experiments including the calculation of economic of the disintegration in laboratory conditions.
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Cellular protein interactions studied by advanced fluorescence imaging methods
Belejová, Sára ; Heřman, Petr (advisor) ; Krůšek, Jan (referee)
This thesis studies an important tumor suppressor, p53, and its interac�on partner, nucleophosmin (NPM), in living cells. Proteins are studied using fluorescence confocal microscopy techniques such as fluorescence life�me imaging and fluorescence anisotropy measurements. The primary focus of the research is on a specific variant of the p53 protein called p53-L344P, which is generated by a point muta�on from its original form (p53wt). We inves�gate the oligomeriza�on state of p53-L344P in vivo, which appears to be monomeric, confirming the results of in vitro experiments from other studies. Further, we show that p53wt and p53-L344P can form complexes with each other. We compare the interac�on of the NPMmutA protein with p53wt and p53-L344P proteins. Our findings reveal that the L344P mutant is not transferred from the nucleus to the cytoplasm in the presence of NPMmut, as is p53wt. Furthermore, we inves�gate the oligomeriza�on state of p53wt when it is in the cytoplasm and propose avenues for further research into this interac�on.
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Tracking membrane permeabilization on single lipid vesicles - method development.
Gücklhorn, David ; Šachl, Radek (advisor) ; Heřman, Petr (referee)
Protein complexes are challenging systems to study, especially when these complexes form on lipid membranes only for a short period of time. This is also the case of fibroblast growth factor 2 (FGF2), a protein that has many physiological and pathological functions in the human organism. It plays major role in the development of cancer as it promotes cell survival and angiogenesis. It also serves as a basis for development of novel treatments of nerve injuries. Despite being heavily studied for many years, it remains unclear how the protein is translocated into the extracellular space where it performs its function. To study complex systems such as FGF2 that self-assembles on the membrane into membrane penetrating pores we decided to develop a simple and efficient fluorescent microscopy method. This method is called double leakage single GUV assay (DLSGA). It utilizes giant unilamellar vesicles (GUVs) mimicking native cellular membranes. In a single experiment, up to 300 individual GUVs are imaged for the content of a leakage dye that reports on the presence of FGF2 pores. During three measurements and under different conditions, detailed information about pore-opening dynamics is gained for each GUV. Results of these measurements are then used to divide GUVs into six groups based on formation and...
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Properties and interactions of nucleoproteins studied by advanced lifetime-based imaging methods
Vacková, Anežka ; Heřman, Petr (advisor) ; Krůšek, Jan (referee)
The main aim of this thesis was implementation and testing of an additional imaging method for investigation of protein-protein interactions in our laboratory and to extend already established heteroFRET (Förster resonance energy transfer) method used with the fluorescence lifetime imaging (FLIM). We performed homoFRET experiments with both the steady-state and time-resolved anisotropy-imaging methods. The presence of homoFRET between fluorophores was done by partial fluorophore bleaching and by the "red edge" analysis. We compared the methods, especially their sensitivity, experimental requirements, complexity, and informational value. We effectively applied steady-state and time-resolved anisotropy imaging to six proteins of interest; both proved comparably effective and reliable as heteroFRET-FLIM for detection of the protein interactions. The red edge method appeared problematic due to background contribution, which could not be suppressed with the current setup and instrumentation. We validated steady-state and time-resolved anisotropy imaging by studying well- described oligomerizing nucleophosmin variants (NPMwt and NPMmut) and noninter- acting mutant NPMcut, expressed in living HEK-293T cells. Then, we also probed nu- cleolin (NCL) and free fluorescent tags NowGFP and mVenus, aiming to find...
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