|
|
Information Strategy
Kratochvíl, Tomáš ; Dědič, Roman (referee) ; Koch, Miloš (advisor)
This diploma thesis is focused on the possibility of using modern approaches to information technology management. Specifically, it concentrates on demands for innovation of software development lifecycle management. The theoretical part of the thesis is focused on methods used in information strategy. Based on results of the analytical part, where I evaluate current approach to information technologies service management, I suggest solutions in the form of customer oriented information strategy and approach to the management of software development as a part of service lifecycle.
|
|
|
Spectroscopic study of verteporphin in liposomes
Laubrová, Veronika ; Dědic, Roman (advisor)
The diploma thesis examined the photochemical properties of verteporphin in liposomes. Absorption and fluorescence spectroscopy and time-resolved detection of singlet oxygen generated by verteporphin were used. The kinetics of singlet oxygen emission were studied under conditions of varying concentrations of dissolved oxygen, the presence of heavy water, and a specific quencher - sodium azide. It was found that verteporphin in liposomes is present in two distinct groups. It was also found that verteporphin in liposomes undergoes a photochemical change, and the formation of a photoproduct was observed in absorption and fluorescence spectra. The formation of the photoproduct is reflected in the times of increase and decrease of the kinetics of singlet oxygen luminescence. It has been demonstrated that the photoproduct is formed in the presence of oxygen in the ground state by an electron transfer mechanism from the triplet state of verteporphin and the formation and subsequent reaction of a superoxide radical.
|
|
|
1DOF helicopter model
Dědič, Roman ; Veselý, Libor (referee) ; Pohl, Lukáš (advisor)
This thesis deals with the design of a 1DOF helicopter fixture and its mathematical model. An important part of this thesis is the identification of the parameters of the physical system and subsequent verification by simulation. Another part is the implementation of the BLDC motor control that was used on the physical fixture. The last part of the thesis deals with the design of the pitch attitude control of the 1DOF helicopter arm. PID controllers and sliding mode control are used here. All control methods were simulated and then verified on the fixture. Hardware operation was performed using an STM microprocessor and Simulink software.
|
|
|
Spectroscopic study of verteporphin in liposomes
Laubrová, Veronika ; Dědic, Roman (advisor) ; Mojzeš, Peter (referee)
The diploma thesis examined the photochemical properties of verteporphin in liposomes. Absorption and fluorescence spectroscopy and time-resolved detection of singlet oxygen generated by verteporphin were used. The kinetics of singlet oxygen emission were studied under conditions of varying concentrations of dissolved oxygen, the presence of heavy water, and a specific quencher - sodium azide. It was found that verteporphin in liposomes is present in two distinct groups. It was also found that verteporphin in liposomes undergoes a photochemical change, and the formation of a photoproduct was observed in absorption and fluorescence spectra. The formation of the photoproduct is reflected in the times of increase and decrease of the kinetics of singlet oxygen luminescence. It has been demonstrated that the photoproduct is formed in the presence of oxygen in the ground state by an electron transfer mechanism from the triplet state of verteporphin and the formation and subsequent reaction of a superoxide radical.
|
|
| |
|
| |
|
|
Polystyrene photoactive nanomaterials producing singlet oxygen
Henke, Petr ; Mosinger, Jiří (advisor) ; Dědic, Roman (referee) ; Kolářová, Hana (referee)
The increasing number of multidrug-resistant strains of bacteria call for alternatives to antibiotic therapy and, more generally, for the antimicrobial material as a component of prevention. Of particular interest is the photodynamic inactivation of bacteria and other pathogens caused by photogenerated singlet oxygen. This work is focused on the field of photoactive polymer nanofiber membranes and nanoparticles, generating singlet oxygen, suitable for medical applications. We prepared different types of photoactive modified polystyrene nanofiber membranes with encapsulated or externally bound porphyrin photosensitizers. These materials efficiently produce highly reactive and cytotoxic singlet oxygen capable of restricted diffusion into to the external environment. Our results demonstrate the crucial role of wettability for materials of this type with a short diffusion length of generated singlet oxygen, illustrate the effect of temperature and indicate their potential use as multifunctional materials. Due to their antimicrobial properties, these materials are suitable alternative to antibiotics and local antiseptics. With good breathability and short diffusion length of singlet oxygen good results can be expect in in vivo tests. From these nanofiber materials we also prepared photoactive extremely...
|
|
|
In situ Luminescence Detection of Singlet Oxygen
Vyklický, Vojtěch ; Dědic, Roman (advisor) ; Mojzeš, Peter (referee)
The thesis summarises already published results of singlet oxygen luminiscence detection in relation to photodynamic therapy treatment. The spectroscopic and photosensitising properties of the photosensitizer TMPyP and their comparison with already published properties of TPPS4 are presented. Also the inuence of phosphatidylcholine on photosensitising properties of TPP and singlet oxygen lifetime was investigated. The thesis is mainly focused on adaptation and optimisation of experimental setup for singlet oxygen luminescence deteciton from the surface of solid samples with time and spectral resolution using optical fibres with luminescence probe. The usability was tested on polymer matrix (PMMA or polystyrene) containing protoporphyrine IX. Finally, the kinetics of singlet oxygen phosphorescence measured from surface of thin layer of 3T3 murine fibroblasts containing TPPS4 or TMPyP are presented. Thereby the feasibility of singlet oxygen detection in situ was demonstrated using this setup.
|
|
|
Fluorescence Detection of Skin Tumours
Stíbal, Adam ; Dědic, Roman (advisor) ; Hála, Jan (referee)
This thesis discusses the photodynamic therapy (PDT) and it's application for dia- gnostics of tumours. The PDT is based on production of reactive oxygen species (ROS) from the molecules of photosensitizers (PS). PS are preferentially accumulated in disea- sed tissues, where ROS are produced and cause selective destruction of the target tissue, while surrounding healthy tissue remains intact. The photodynamic diagnostics uses the fluorescence of PS for digital and spectral imaging. This thesis is focused on detection of tumours from spectral characteristics of protoporphyrin IX fluorescence induced in tis- sue by methyl-aminolevulinate. The main part of the research was realised on rats' scars in vivo, the solutions of homogenized mouse fibroblasts were also studied. The way to distinguish between diseased and healthy tissue was found using measurements of fluo- rescence bleaching kinetics.
|
|
| |
guest ::
RSS feed.