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Molecular and epidemiological characteristics of Burkholderia cenocepacia strain dominant in cystic fibrosis patients in the Czech Republic
Vilimovská Dědečková, Klára ; Dřevínek, Pavel (advisor) ; Macek, Milan (referee) ; Šmajs, David (referee)
Summary: Bacteria from BurkJwlderia cepacia complex represent the most serious pathogens for patients witb cystic fibrosis. l nc:ir scverity IS caused by their antibiotic resistance and ability to Sllrcad among patients wilh cystic fibrosis. Many cyslic fibrosis cenlrcs around IlIe world, also tbe biggest Czech Cystic Fibrosis Centre in University Hospital in Motol, faeed outbreak. In Ihe 1990's tlle epidernie strain HurMwlderia cenocepacia S1'32 spread in a population of cystic fibrosis patienlS. One of the goals of the tunent thesis was to study epidemiological situation of Blďkholderia cepada oornplex in the popolation of eystie fibrosis pmients allending Cysfie Fibrosis Centre in University Hospilal MOlol almost seventeen years after first cases of infected palients wilh Bec inrection. Otber goals were: to evaloate 3 usefulness of molccular genelie diagnosties of Burkholderia cepacia complex tetl years afier ils implementation in routine diagnosties, to improve surveillal1ee syslem not only by implementalion or simple and specifie tesl for detection of epidemie stmin, bul 3150 by implementation of genolyping melllods thal are able to detennine strain in olher cases of infeetion. The [asI goal was to characterize Ibe epidemie slrain frolll virolence point ofview. Dased oRthe thesis we: confirmed...
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Application of modern technologies for treatment of biological samples for HPLC analysis.
Dědečková, Klára ; Solich, Petr (advisor) ; Karlíček, Rolf (referee)
This diploma thesis is dealing with a using of new technologies for the treatment of biological material in the HPLC analysis. The main aim of this thesis was miniaturization of solid phase extraction process of blood serum on classical SPE columns to SPE plates for determination of liposoluble vitamins A and E by high performance liquid chromatography. The new extraction method for vitamin A and E in blood serum using the modern format of SPE plate was developed and optimized. This new method mineralizes a blood serum volume, makes sample treatment easy and can speed up the analysis.
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Molecular and epidemiological characteristics of Burkholderia cenocepacia strain dominant in cystic fibrosis patients in the Czech Republic
Vilimovská Dědečková, Klára ; Dřevínek, Pavel (advisor) ; Macek, Milan (referee) ; Šmajs, David (referee)
Summary: Bacteria from BurkJwlderia cepacia complex represent the most serious pathogens for patients witb cystic fibrosis. l nc:ir scverity IS caused by their antibiotic resistance and ability to Sllrcad among patients wilh cystic fibrosis. Many cyslic fibrosis cenlrcs around IlIe world, also tbe biggest Czech Cystic Fibrosis Centre in University Hospital in Motol, faeed outbreak. In Ihe 1990's tlle epidernie strain HurMwlderia cenocepacia S1'32 spread in a population of cystic fibrosis patienlS. One of the goals of the tunent thesis was to study epidemiological situation of Blďkholderia cepada oornplex in the popolation of eystie fibrosis pmients allending Cysfie Fibrosis Centre in University Hospilal MOlol almost seventeen years after first cases of infected palients wilh Bec inrection. Otber goals were: to evaloate 3 usefulness of molccular genelie diagnosties of Burkholderia cepacia complex tetl years afier ils implementation in routine diagnosties, to improve surveillal1ee syslem not only by implementalion or simple and specifie tesl for detection of epidemie stmin, bul 3150 by implementation of genolyping melllods thal are able to detennine strain in olher cases of infeetion. The [asI goal was to characterize Ibe epidemie slrain frolll virolence point ofview. Dased oRthe thesis we: confirmed...
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Application of modern technologies for treatment of biological samples for HPLC analysis.
Dědečková, Klára ; Karlíček, Rolf (referee) ; Solich, Petr (advisor)
This diploma thesis is dealing with a using of new technologies for the treatment of biological material in the HPLC analysis. The main aim of this thesis was miniaturization of solid phase extraction process of blood serum on classical SPE columns to SPE plates for determination of liposoluble vitamins A and E by high performance liquid chromatography. The new extraction method for vitamin A and E in blood serum using the modern format of SPE plate was developed and optimized. This new method mineralizes a blood serum volume, makes sample treatment easy and can speed up the analysis.
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