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QoS Implementation in Access Network
Adámek, David ; Molnár, Karol (referee) ; Filka, Miloslav (advisor)
This diploma thesis deals with computer networks which play a key role in present society. More and more demands are placed on these networks (especially on quality of services and throughput) because of fast-growing developement of services such as voice and video real-time transmissions. All the packets in typical Ethernet networks are equal. Therefore it is sometimes impossible for the application to maintain required throughput speed of a network. For this case it is useful to take some mechanisms into the consideration. These mechanisms (sometimes called QoS) are able to distinguish different types of transmissions and traffic, analyze them and then prioritize them according to some pre-defined set of rules. The most famous mechanisms are Integrated Services and Differentiated Services. The implementation of Differentiated Services is the goal of this thesis.
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Immunomodulation using two types of ligands
Adámek, David ; Bezouška, Karel (advisor) ; Pompach, Petr (referee)
CD69 and NKR-P1 are receptors belonging to C-lectin family of receptors. We have recently identified two types of effective ligands for these receptors: dimeric N-acetyl-D-glukosamines (activators) and (calix[4]arenes) inhibitors. By using them, we can modulate immune response. First class of ligands (N-acetyl-D-glukosamines) is able to bind to CD69 and NKR-P1 and activate the cells. Second class of ligands (calix[4]arenes) is able to effectively inhibit functions of CD69, but not functions of NKR-P1. The aim of this study was to learn how to modulate immune response by various combinations of these compounds, and to explore their concentrations suitable for tumor therapies. Effect of used ligand and their combinations was studied in standard tests of cell proliferation, cell death and cytotoxic killing.
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Preparation of recombinant forms of the extracellular part of mouse leukocyte receptors from NKR-P1 family.
Adámek, David
Mouse NK cell receptors belonging to NKR-P1 family plays role in activation, inhibition and cytokine secretion by these cells. Aim of this thesis is preparation of extracellular parts of C57BL/6 mouse strain activating receptors mNKR-P1A and mNKR-P1C. Production vectors with coding sequences of both proteins were prepared. Next, optimization of production in E. coli was done and appropriate in vitro refolding and purification protocol were developed. Purified proteins were characterized by mass spectrometry and labeled by a fluorescent dye. Primary screening for potential ligand was performed. Further work will involve structural characterization of the receptors and identification of their ligands. These data may help to clarify the function of NK cells.
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Preparation of recombinant forms of the extracellular part of mouse leukocyte receptors from NKR-P1 family.
Adámek, David
Mouse NK cell receptors belonging to NKR-P1 family plays role in activation, inhibition and cytokine secretion by these cells. Aim of this thesis is preparation of extracellular parts of C57BL/6 mouse strain activating receptors mNKR-P1A and mNKR-P1C. Production vectors with coding sequences of both proteins were prepared. Next, optimization of production in E. coli was done and appropriate in vitro refolding and purification protocol were developed. Purified proteins were characterized by mass spectrometry and labeled by a fluorescent dye. Primary screening for potential ligand was performed. Further work will involve structural characterization of the receptors and identification of their ligands. These data may help to clarify the function of NK cells.
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Preparation of recombinant forms of the extracellular part of mouse leukocyte receptors from NKR-P1 family.
Adámek, David ; Man, Petr (advisor) ; Brdička, Tomáš (referee)
Mouse NK cell receptors belonging to NKR-P1 family plays role in activation, inhibition and cytokine secretion by these cells. Aim of this thesis is preparation of extracellular parts of C57BL/6 mouse strain activating receptors mNKR-P1A and mNKR-P1C. Production vectors with coding sequences of both proteins were prepared. Next, optimization of production in E. coli was done and appropriate in vitro refolding and purification protocol were developed. Purified proteins were characterized by mass spectrometry and labeled by a fluorescent dye. Primary screening for potential ligand was performed. Further work will involve structural characterization of the receptors and identification of their ligands. These data may help to clarify the function of NK cells.
|
|
|
Immunomodulation using two types of ligands
Adámek, David ; Pompach, Petr (referee) ; Bezouška, Karel (advisor)
CD69 and NKR-P1 are receptors belonging to C-lectin family of receptors. We have recently identified two types of effective ligands for these receptors: dimeric N-acetyl-D-glukosamines (activators) and (calix[4]arenes) inhibitors. By using them, we can modulate immune response. First class of ligands (N-acetyl-D-glukosamines) is able to bind to CD69 and NKR-P1 and activate the cells. Second class of ligands (calix[4]arenes) is able to effectively inhibit functions of CD69, but not functions of NKR-P1. The aim of this study was to learn how to modulate immune response by various combinations of these compounds, and to explore their concentrations suitable for tumor therapies. Effect of used ligand and their combinations was studied in standard tests of cell proliferation, cell death and cytotoxic killing.
|
|
|
QoS Implementation in Access Network
Adámek, David ; Molnár, Karol (referee) ; Filka, Miloslav (advisor)
This diploma thesis deals with computer networks which play a key role in present society. More and more demands are placed on these networks (especially on quality of services and throughput) because of fast-growing developement of services such as voice and video real-time transmissions. All the packets in typical Ethernet networks are equal. Therefore it is sometimes impossible for the application to maintain required throughput speed of a network. For this case it is useful to take some mechanisms into the consideration. These mechanisms (sometimes called QoS) are able to distinguish different types of transmissions and traffic, analyze them and then prioritize them according to some pre-defined set of rules. The most famous mechanisms are Integrated Services and Differentiated Services. The implementation of Differentiated Services is the goal of this thesis.
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