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Biofunctionalization of water soluble CdTe quantum dots
Šibíková, Anna ; Drbohlavová, Jana (referee) ; Pekárková, Jana (advisor)
This bachelor thesis is focused on the research on biofuncionalization of water-soluble glutathione modified CdTe quantum dots (QDs). The thesis is divided into three parts. The first part is a theoretical introduction to the QDs and their synthesis, modifications and applications in biology together with an introduction to proteins and bioconjugation of QDs and BSA. In the second part I present the experimental results of water-soluble glutathione modified CdTe-GSH QDs preparation. The QDs were cleaned and bioconjugated with BSA at different concentrations. This is described in the third part of the thesis. I also discuss the obtained experimental results in the frames of available literature.
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Synthesis of quantum dots for proteins detection
Šibíková, Anna ; Fohlerová, Zdenka (referee) ; Pekárková, Jana (advisor)
This thesis is focused on synthesis of quantum dots (QDs) for protein detection. It comprises three parts. The first part summaries the theory of QDs, their synthesis, functionalization, interactions and applications in medicine. In the second part synthesis of CdTe/ZnS core/shell QDs modified by glutathione (GSH) is described, followed by the conjugation with biomolecules BSA and IgG. Several coupling agents such as EDC with NHS and CDI were used. In the last part, the final products were characterized by fluorescence spectroscopy and capillary electrophoresis. The results show the dependence of the fluorescence intensity of the QDs on pH range, concentration of BSA and IgG concentrations using different crosslinkers.
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Biofunctionalization of water soluble CdTe quantum dots
Šibíková, Anna ; Drbohlavová, Jana (referee) ; Pekárková, Jana (advisor)
This bachelor thesis is focused on the research on biofuncionalization of water-soluble glutathione modified CdTe quantum dots (QDs). The thesis is divided into three parts. The first part is a theoretical introduction to the QDs and their synthesis, modifications and applications in biology together with an introduction to proteins and bioconjugation of QDs and BSA. In the second part I present the experimental results of water-soluble glutathione modified CdTe-GSH QDs preparation. The QDs were cleaned and bioconjugated with BSA at different concentrations. This is described in the third part of the thesis. I also discuss the obtained experimental results in the frames of available literature.
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Synthesis of quantum dots for proteins detection
Šibíková, Anna ; Fohlerová, Zdenka (referee) ; Pekárková, Jana (advisor)
This thesis is focused on synthesis of quantum dots (QDs) for protein detection. It comprises three parts. The first part summaries the theory of QDs, their synthesis, functionalization, interactions and applications in medicine. In the second part synthesis of CdTe/ZnS core/shell QDs modified by glutathione (GSH) is described, followed by the conjugation with biomolecules BSA and IgG. Several coupling agents such as EDC with NHS and CDI were used. In the last part, the final products were characterized by fluorescence spectroscopy and capillary electrophoresis. The results show the dependence of the fluorescence intensity of the QDs on pH range, concentration of BSA and IgG concentrations using different crosslinkers.
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