National Repository of Grey Literature 368 records found  1 - 10nextend  jump to record: Search took 0.06 seconds. 

Polymerase synthesis of modified DNA containing cytosine in major groove
Kielkowski, Pavel ; Hocek, Michal
(Cytosine-5-yl)ethynyl derivatives of pyrimidine and 7-deazaadenine 2-deoxyribonucleosides and nucleoside triphosphates (dNTPs) were prepared by aqueous Sonogashira coupling. All modified dNTPs were excellent substrates for DNA polymerases to give DNA bearing acetylene- linked cytosine(s) in the major groove.

The analysis of the regional budget economy in the Czech Republic and application on the capital city of Prague
Dušátková, Klára ; Peková, Jitka (advisor) ; Matula, Miloš (referee)
The aim of this thesis is to investigate the management of budgets in the regions of the Czech Republic and in the capital city of Prague between the years of 2003 and 2013. Comparison is made between the capital city of Prague, Central Bohemian Region and South Moravian Region. The thesis opens with description of historical and legislative framework and continues with strong reference to the theory of public finance. The research shows that the most important source of revenues for the regions are financial transfers. Majority of Prague's income comes from public tax revenues. Current expenditures are the most prominent outlay of the regions and of Prague, too. The regional and Prague's total debt has grown continuously over the researched period. Strong impact on the budget economy has had the global economic crisis which has influenced the budgets since 2009.

Determination of municipal fire protection units in the territory of South Bohemia region to perform task in the field of protection of population.
ŘIMNÁČ, Jiří
In order to preserve its existence since its origination, mankind has had to deal with a variety of adverse effects which have been largely influencing human conduct and behaviour. We become witnesses to events that are identified as exceptional, because they often occur unexpectedly and endanger the lives and health of people. These events cause great damage to property and the environment. They may arise from harmful reactions of forces and phenomena resulting from human activities, from natural forces or from a combination of both. Such events may be floodings, storms, technological accidents with the release of hazardous substances, major road accidents, extensive fires, acts of terrorism. The management of such incidents and emergencies requires the intervention of the Integrated Rescue System. The joint intervention of the components abides by legal standards and internal guidelines. During the intervention of the Integrated Rescue System, a crucial part of the activities is carried out by the Fire and Rescue Units. The diploma thesis deals with the legislation in the field of civil protection and population protection. Information on the history of civil protection and population protection tasks is provided. The administrative territory of the South Bohemian region is characterized. The issue of the Integrated Rescue System and its structure is dealt with. The Fire and Rescue Units are proposed as appropriate to protect the population.

Comparative evaluation of protein composition in human breast cancer cells using mass spectrometry
Flodrová, Dana ; Toporová, L. ; Macejová, D. ; Laštovičková, Markéta ; Brtko, J. ; Bobálová, Janette
Bottom-up proteomic approach was used for detailed characterization of proteins from\ntwo human tumour cell lines representing major clinically different types of breast\ncancer. The aim was to show the differences between them on proteomic level. Here\nwe present almost 100 unequivocally identified proteins out of which 60 were mutually\ndifferently expressed for MDA-MB-231 and MCF-7. Some well-known breast cancer\nmarkers like annexins A1, A2 and vimentin were found in the MDA-MB-231 cell line.\nOn the other hand, MCF-7 cells were found to be positive for cytokeratins and keratins\nand thus we were able to distinguish both cell lines sufficiently.

Kontextové modely pro statistickou kompresi dat
Paška, Přemysl ; Žemlička, Michal (referee) ; Dvořák, Tomáš (advisor)
Current context modelling methods use an aggregated form of the statistics reusing the data history only rarely. This work proposes two independent methods that use the history in a more elaborate way. When the Prediction by Partial Matching (PPM) method updates its context tree, previous occurrences of a newly added context are ignored, which harms precision of the probabilities. An improved algorithm, which uses the complete data history, is described. The empirical results suggest that this PPM sub-obtimality is one of the major cause of the problem of inaccurate probabilities in high context orders. Current methods (especially PAQ) adapt to non-stationary data by strong favoring of the most recent statistics. The method proposed in this work generalizes this approach by favoring those parts of the history which are the most relevant to the current data, and its imlementation provides an improvement for almost all tested data especially for some samples of non-stationary data.

Bohemian lands and the War of 1813
Kessler, Vojtěch ; Bělina, Pavel (referee) ; Stellner, František (advisor)
In my work I try to analyze the war events of the year 1813, which took place on our land. I focused mostly on the reactions to the events from the inhabitants of the cities and the countryside. Therefore the key questions were - how did the inhabitants see and understand the major war events of their time. I also kept in mind the reflection about the economical difficulties caused by the military actions, or by a simple passing of the armies. For the obvious reasons I focused on the inhabitants of the north of the Bohemia region. It turned out that the war is still understood by a simple man of the beginning of the 19 century as a God's deal or as a caprice of the mighty. The people realized it's destructive potential only if it directly affected them. We could say that the level of the disagreement and the laments is inversely proportional to the distance between the places where the battles took place or where the armies were passing and the residences of the people manifesting them. Despite the sufferings of the war and the economic exhaustion, very soon after the battles had stopped, news about the natural cycles and the harvest started to reappear in the chronicles. Those were undoubtedly the events of a great importance for a person of that time.

Differentiation of offers and methods of working physiotherapy of persons with a mental or combined handicap in a practice of social service providers and another subjects.
POŠMUROVÁ, Nikola
In the Bachelor's thesis I have dealt with differentiation of tenders and rehabilitation methods. Specifically, individuals with mental or multiple disabilities and for providers of social services and other bodies. In the theoretical part I focused on defining the basic terms relating to that issue. In the introductory chapter I dealt with the definition of the concept of mental disability, causes of its creation and definition of degrees of mental retardation. The next chapter is devoted to the combined disability, specifically the yoke of the definition, causes of and classification. In the third chapter is devoted to the importance of the work, the status of the work in a person's life and the status of work for persons with disabilities, including individuals with mental or multiple disabilities in Bachelor thesis I. In the next chapter, I deal with comprehensive rehabilitation system, specifically the remedies rehabilitation, educational resources, social resources, rehabilitation, rehabilitation and special attention devoted work resources, rehabilitation, which will directly affect the Bachelor thesis. Furthermore, I determine a target group the work of rehabilitation and the preconditions for its implementation. In other chapters is devoted to the specification of tools and resources work rehabilitation, employment policy tools and I define jobs that are offered to persons with intellectual disabilities. In the practical part of the thesis, first determine the main aim and target work. The main goal was to determine what are the menus and methods of rehabilitation for people with mental or multiple disabilities with providers of social services. As the target I had set to determine what are the possibilities of transmission from individual to social services on the open employment market. Specifically, I've focused on the resources provided by the rehabilitation of daily, weekly care centers or day service center, in addition to the socio-therapeutic workshops, in a sheltered employment, and employment on the open employment market. To meet the objectives I used a qualitative approach, specifically the semi-structured interview with six providers of social services of the South Bohemian region The analysis of the information obtained, I discovered that if the device is operated daily, weekly social welfare institution or Centre, day services, the services provided are identical, not different from each other. In the case of socio-therapeutic workshops is the diversity of the wider, each device provides a variety of services, different options, some devices have socio-therapeutic workshops directly on the device, others have socio-therapeutic workshops and externally, outside of your device. Protected jobs are also in the majority of providers of social services offered both within and outside the device, always the odd jobs, such as in the kitchen, cleaning, in teahouses and the like. It always depends on the type and degree of disability, on the basis of a levy for the client to the appropriate activities. Location of the client on the open employment market is not very common in most devices, some devices that offer clients or do not provide. Partial objective was to detect transmission, I found that the only limiting factor is the client's disability that limits its options and device options, of course, that the services it offers and delivers. The aim of all devices is to achieve the greatest possible degree of autonomy of the client and allow it to advance from care centers or day service center in the socio-therapeutic workshops, found a protected job, and a job on the open employment market.

Interaction of heteroboranes with biomolecules: Accurate quantum chemical study
Fanfrlík, Jindřich ; Hobza, Pavel (advisor) ; Fišer, Jiří (referee) ; Otyepka, Michal (referee) ; Vondrášek, Jiří (referee)
Charles University in Prague Faculty of Science Department of Physical and Macromolecular Chemistry Interaction of heteroboranes with biomolecules: Accurate quantum chemical study Doctoral Thesis Abstract RNDr. Jindřich Fanfrlík Advisor: Prof. Ing. Pavel Hobza, DrSc. Institute of Organic Chemistry and Biochemistry, AS CR Center for Biomolecules and Complex Molecular Systems Prague 2008 Univerzita Karlova v Praze Přírodovĕdecká Fakulta Katedra fyzikální a makromolekulární chemie Interakce heteroboranů s biomolekulami: kvantovĕ chemická studie Autoreferát disertační práce RNDr. Jindřich Fanfrlík Školitel: Prof. Ing. Pavel Hobza, DrSc. Ústav organické chemie and biochemie, AV ČR Centrum biomolekul a komplexních molekulových systémů Praha 2008 Introduction The world wide epidemic of AIDS is caused by two species of the human immunodeficiency virus (HIV-1 and HIV-2). Its 9200-bases-long RNA genome contains three main genes: gag, pol and env. The enzymes located within the pol gene (protease PR and reverse transcriptase RT) have become major targets for drug discovery, e.g. RT inhibitor Tenofovir disoproxil fumarate (DF) discovered by A. Holy at UOCHB in Prague and manufactured by Gilead Sciences and also already ten HIV-1 PR inhibitors (PIs) have been approved for use in the clinics: The evolution of drug...

Environmental Impact Assessment Follow up analysis of Pankrác administrative centre
Franclová, Alena ; Keken, Zdeněk (advisor) ; Karel, Karel (referee)
This diploma thesis evaluates issues of environmental impact assessment, focusing on post-project analysis of the Pankrác administrative center. Follow-up evaluation is one of the most important practice through EIA which can fulfil one's potential. Major contribution is mainly the EIA feedback which will be compared between real and predicted impact. Follow-up will provide knowledge to avoid possible non-conformances in EIA evaluation process in the future. Methodical of this thesis is based on international standards of good practice in EIA follow-up, which should always correspond with the specific circumstances of the project and the specific context. The main principles are results-oriented approach, learning, institutional commitment and responsibility. Meaning of the follow-up is the main research question to evaluate the environmental impact assessment. The thesis evaluates the implementation of the main comments from the various point of views. All opinions, changes and views of all participants must be reviewed in implementation and operation phases of the EIA process. Another sub-section provides a comparison of the noise pollution before and after realization of the project and analytical part concludes evaluate compliance with the general principles of good practice. The results collect comprehensive assessment of the EIA process. The follow-up for Pankrác administrative center was important and it proved that positive cooperation with the investor made processes more effective in the terms of the environmental protection of the area. In the future it would be appropriate to dedicate more effort and time for the follow-up. Especially raising awareness of the existence and positive impact of the entire EIA process and also to protect the environmental and public health.

Changes in the business environment of Ukraine in the last decade and the possibility of cooperation with the Czech Republic
Tarasenko, Mariya ; Zamykalová, Miroslava (advisor) ; Krčál, Adam (referee)
The aim of this thesis is to analyze the business environment of Ukraine from year 2001 to 2011 and evaluate the major changes and trends. I will proceed from the basic assessment of the economic environment in Ukraine to its focus on business issues. At the beginning I will show the development of general macroeconomic indicators of Ukrainian economy, then I will give statistics on international trade and investment. The next section will evaluate the international trade of Ukraine with its main partners. After that I will focus on the possibilities of cooperation with Czech Republic. At the end I will show specific actions related to the establishment of a foreign legal entity. Based on the analysis and my own experience I will create recommendations for entrepreneurs wishing to start working and develop business in Ukraine. I will also make some proposals for improvements to be made in the business environment in Ukraine to ensure effective trade with foreign business partners. The result of this thesis should be the conclusion if Ukraine is moving in the right direction nowadays.