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Financial analysis of the industrial enterprise
HŘEBEČKOVÁ, Darina
Financial analysis is the important device of assessing the financial situation of enterprise on the basis of the financial statements for several periods. It serves for decision making for both owners and shareholders, and banks and state assemblies. After the performed financial analysis the enterprise should be able to decide about the use of free funds, about its structure of assets and about the way of the funding to the future. The theoretical part deals with the aim and the object of the financial analysis, users of the financial analysis and techniques for evaluation of the financial health of enterprise. The practical part determines financial ratios of the financial analysis, such as profitability of the enterprise or solvency, applied on the industrial enterprise. The aim of the thesis is to evaluate the development of the financial situation in the selected industrial enterprise, identify the potential sources of the inefficiency and propose the measures for their elimination.
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Development of Meta-Server for Prediction of Mutations Effects on Protein Function
Lisák, Peter ; Burgetová, Ivana (referee) ; Jaša, Petr (advisor)
This bachelor thesis deals with analysis of genomic data, more specifically prediction of effects of mutations on protein function using a protein sequence or tertiary structure. The theoretical introduction describes the basics of genetics and bioinformatics and is followed by description of selected prediction tools such as SIFT, MAPP and AUTO-MUTE. A unified interface for work with different tools is proposed in the thesis. The meta-server interface allows running a computation and collecting results from one site. meta-server combines results of implemented tools and provides a consensual prediction, which is expected to be more accurate than the results from individual tools. Finally, testing of meta-server on the real data and comparisons of predictions with the experimentally obtained results are presented.
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Experiment CAPEX-U: Současnost a Budoucnost
Frolov, Oleksandr ; Koláček, Karel ; Štraus, Jaroslav ; Schmidt, Jiří ; Boháček, Vladislav ; Prukner, Václav
A new pulse capillary discharge driver with a laser-triggered main spark gap has been developed. The assembled CAPEX-U device consists of a Marx generator, of a pulse forming line, a gas-filled laser-triggered spark gap and a ceramic capillary. Its design was supported by mapping of electrostatic fields in the most exposed regions and by analysis of an equivalent circuit. Operation of the laser-triggered spark gap was tested on a single-channel model. In the paper, the results of the modelling and of the first electrical tests of the whole device (capillary current and voltage at the end of pulse forming line) are presented.
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Characterization and stabilization of pancreatin
Wurstová, Agáta ; Němcová, Andrea (referee) ; Obruča, Stanislav (advisor)
This work focuses on a study of enzyme mixture pancreatin, its characterization and subsequent encapsulation into liposomes. As a reference proteins bovine serum albumin and trypsin were used. Characterization of pancreatin consisted of two parts. The first part focuses on optimization of methods for the concentration determination by absorption spectrophotometry using basic methods for identifying proteins (Biuret method, Hartree-Lowry method and Bradford method). Moreover, UV spectrums of the protein were measured. As a method for identification of protein´s molecular weight, SDS-PAGE was used. To identify components of pancreatin, LPLC was employed in two modifications, ion-exchange chromatography and size exclusion chromatography. The second part is dedicated to the characterization of pancreatin as enzyme in terms of pH and temperature optimum for the enzyme activities of protease (pH 9, 8 and 50 °C), amylase (pH 7 and 40 °C) and lipase (pH 7 and 50 °C). The last part of this work aimed at an encapsulation of pancreatin into liposomes and DLS analysis of distribution of particles and their zeta potential. Liposomes did not spontaneously release encapsulated enzyme. To confirm that proteins were successfully entrapped into liposomes, their structure was disrupted by application of phospholipase D. In conclusion, liposomes can be utilized as delivery systems for native enzymes.
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Development of prostate cancer radiotherapy from the 80s to the present day
SUCHANOVÁ, Markéta
Prostate cancer (CaP) remains the most common cancer diagnosed in men. This desease has worldwide increasing incidence. In the Czech Republic incidence has tripled during the last 20 years. The increase of the mortality is much more slower, but CaP is still the third leading cause of cancer-related death in men. CaP treatment management options may include active surveillance, radical prostatectomy, brachytherapy and external beam radiation therapy. The radiotherapy occupies an irreplaceable position. It is used as a curative therapy for clinically localized CaP with a low and intermediate risk. In the case of high risk CaP and locally advanced tumors are often treated with the addition of the hormonal therapy, which enhances tumor control and overall patient's survival. The radiotherapy is also used in some cases as an adjuvant treatment following a radical prostatectomy, as well as a salvage therapy in a biochemical relapse or as a palliative treatment if the cancer progresses. The aim of this study is to provide an overview of the development of CaP radiotherapy from the 80s till the present day. The specific objective of the study is to assess the incidence of the radiation-related complications (RRC) in patients with CaP treated with IMRT and PT. Among the methods of processing this thesis was using secondary data analysis. Practical information were obtained during the visits to the Department of the Radiation Oncology at the Hospital Ceske Budejovice a.s. (NCB) and the Proton Therapy Center Czech s.r.o. (PTC). During these visits was observed the radiotherapeutic treatment of the patients with CaP and valuable information were obtained through interviews with Mrs.Vančurová, MD and Mr. Vítek, MD., Ph.D., MBA This study analysed the incidence of the acute and late RRC in patients with CaP treated in these facilities. The acute and late side effects was assessed for GU and GI toxicity using RTOG / EORTC score. The specific problems were measured with the CTCAE v.4.0 scale. The overall incidence of the RRC was subjected to the statistical hypothesis testing using a two-sample unpaired t-test. The examined sample consisted of the 30 patients with CaP irradiated at NCB and the 30 patients from PTC with low, intermediate and high risk CaP. It was found that for the patients from NCB the rectum mean dose was 40,7 Gy and the mean dose of bladder was 39,4 Gy. For the patients treated at PTC the mean dose to the rectum was 13,2 Gy and to the bladder 11.1 Gy. This is related to the incidence of the RRC. Acute GU and GI toxicities Grade 2+ occurred in 33 % and 23,3 % of the patients treated in NCB. With a median follow-up of 22 months there was late GU toxicity Grade 2+ in 13,3 %. Late GI toxicity Grade 2+ occurred in 26,7 %, which exceeds incidence of the RRC described in the literature. Incidence of late GI toxicity Grade 3 and 4 was also increased, it appeared in 10 % (3 patients). Furthermore in one case cancer reoccurred and metastasized one and half year after the radiotherapy. In PTC no acute or late Grade 3+ toxicities were observed. Acute GU and GI toxicities Grade 2 occurred in 13,3 % and 6,7 %. After median follow-up of 21,5 months there was incidence of late GI toxicity Grade 2 3,3 %, late GU toxicity appeared only Grade 1 in 13,3 %. There was found statistically significant difference in occurrence of RRC in patients treated in NCB and PTC. The hypothesis that PT reduces the incidence of the radiation side-effects compared to the IMRT technique in the patients with the prostate cancer was confirmed. The prostate can move greatly during the day. It is recommended using CBCT, which is part of new linear accelerators at NCB, to check the position of the prostate before each irradiation. This study provides an overview of the treatment of CaP and may serve to increase of awareness of the RRC. The main contribution can be seen in the comparison of a standard approach with a promising alternative.
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Mathematical Modelling of Relation between Economic Quantities
Žigárdy, Martin ; Hřebíček, Jiří (referee) ; Chvátalová, Zuzana (advisor)
My work is aimed on creating and on subsequent economical interpretation of mathematical models presenting relations of continual development of PX index (Czech stock index) and continual developments of five important world titles, which are price of the West Texas Intermediate Crude Oil barrel, exchange rate between Dollar and Czech Crown as well as Euro and Czech Crown and indexes DAX (German stock index) and Dow Jones (American stock index). The models are create by using regression analysis and by mathematical software Maple version 12
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Dynamic modification of proteins for fluorometric detection in CZE
Horká, Marie ; Šlais, Karel
The separation techniques employing fluorescence detection are sensitive and selective so they have been often applied for the trace analysis of biological samples. The commonly used derivatization of proteins can lower the detection limits; however, they can change the acido-basic properties and mobilities when compared to the native species. Recently, we have used the colored tenside as a buffer additive for the photometric detection of proteins in the UV region. The selectivity, efficiency and resolution of CZE separation using this dye were found to be similar to the CZE with SDS as the additive. In this study, the ampiphilic fluorescent compound is suggested as a buffer additive in CZE for dynamic modification of the sample of several proteins. Using the deuterium lamp for the excitation in the UV region for the on column fluorometric detection, the amol minimum detectable amounts of the proteins sampled on the CZE capillary were achieved.
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Design of software single purpose machine for manufacturing test
Janoušek, Roman ; Šubrt, Kamil (referee) ; Houška, Pavel (advisor)
This diploma thesis deals with design and implementation of software for control of manufacturing tests of pneumatic proportional valve Festo MPYE. The single-purpose testing device consists of a DAQ device, a pressure sensor and of electrically operated pneumatic valves. In the introduction of this thesis are outlined aims of thesis followed by introduction to single-purpose assemblies commonly used in practice. The main part of this thesis deals with analysis and with solving the issues of assigned product testing. The designed and implemented modular software for control of manufacturing tests of assigned product is presented as an outcome and this software is easily adjustable for tests of a different product. In the conclusion are assessed achieved aims.
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Transfer of production to China
Janek, Michal ; Halík, Jaroslav (advisor) ; Jezný, Tomáš (referee)
The thesis is focused on transfer of production to China. The aim of the thesis is to evaluate the potential of China from the perspective of companies and investors, and on the example of the international company to highlight not only the traditional ways of entering the Chinese market, but also the specific method that considers the needs of manufacturing companies. Thesis also contains the assessment of competitiveness of the Chinese economy, cultural differences and comparison of the various forms of entering the Chinese market. The practical part is focused on the analysis of enterprise from the polygraphic industry which has transferred part of its assembly processes to China. Conclusion contains recommendations for further development of the company on the Chinese market.
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Prolyl endopeptidase of the blood fluke Schistosoma mansoni
Fajtová, Pavla ; Konvalinka, Jan (advisor) ; Ryšlavá, Helena (referee)
Prolyl endopeptidase SmPEP from the blood fluke Schistosoma mansoni is investigated here for the first time. This enzyme is potentially interesting as a drug target for the treatment of schistosomiasis. SmPEP was detected in the extract of adult worms by enzyme activity and immunoreactivity. Enzymatically active SmPEP was produced in the E. coli expression system and was chromatographically purified. The pH optimum of recombinant SmPEP was about 8. Substrate specificity analysis revealed that SmPEP cleaved peptide substrates by endopeptidase activity, however, macromolecular substrates were not fragmented. The residue preferences in the positions P3 to P1' were determined using synthetic fluorogenic peptide substrates. SmPEP was found to be highly sensitive to the inhibition by Z-Ala-Pro-CMK and Z-Arg-Pro-CHO. Primary screening of crystallization conditions for recombinant SmPEP was performed. " (In Czech)"
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