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Determination of optimal conditions for cell growth and accumulation of storage lipids of the alga Trachydiscus minutus
ONDREJMIŠKOVÁ, Kamila
Algae are becoming a strategic source of fuels, food, feedstocks, and biologically active compounds. Algal lipids are among the most promising potential products. The yellow-green alga Trachydiscus minutus (class Eustigmatophyceae) is unicellular freshwater alga accumulating lipids as its storage reserves. The influence of various growth média, temperature and light intensity on the growth and accumulation of storage lipids of alga Trachydiscus minutus was studied. The optimal growth conditions were determined. Storage lipids were quantitatively determined fluorometrically. Accumulation of lipids was monitored by fluorescence microscopy.
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Possibilities for bioindication of the surface water status using freshwater bivalves’ shells with focus on the morphological variability of Unio crassus
Zuzáková, Hana ; Douda, Karel (advisor) ; Matasová, Klára (referee)
Possibilities for bioindication of the surface water status using freshwater bivalves shells with focus on the morphological variability of Unio crassus
Summary
This bachelor thesis present a review of scientific literature dealing with the possibilities for bioindication by freshwater mussels of the family Unionidea. In it practical part, it is about the variability of freshwater mussel Unio crassus, whose shells were collected from 10 streams in Poland, Bulgaria and Romania. Specifically, shells from 357 U. crassus individuals were measured by classical morphometric method. This analysis confirmed findings from literature where are mentioned most common size of shells (40- 70 mm of length). Using residual values of the relationship between shell length and width and between shell length and the thickness the shell shapes were compared between individuals and the watersheds with the use of basic statistical methods, scatterplots and boxplots.
The result is a significant value for different flows, their differences are only in the few millimeter variance. For a substantial variation in shapes were made GIS analysis for each river. To describe the catchment area were delineated buffers in the size of square root lengths of streams, in which was to determine land-use and their percentage and surface representation. Land-use categories were characterized by Corine Land Cover classes. Identified categories in studied areas include total afforestation total artificially built-up areas, agricultural and water areas.
The resulting statistics of land use were compared and evaluated in relation to the shape characteristics of U. crassus and enabled the determination of species plasticity and variability which is capable, despite their demands and the need for high quality waters, live in diverse environmental conditions. Despite the limited set of population tested, study documents that typical environmental conditions for U. crassus are unpolluted areas with a high percentage of afforestation, the average representation of agricultural land, and the lowest representation of any built-up areas and industrial application in the nearby stream.
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Water quality valuation (case study of Máchovo jezero)
Škopková, Hana ; Ščasný, Milan (advisor) ; Kameníček, Jiří (referee)
This thesis estimates the benefits of water quality improvement on Macha Lake using the contingent valuation method. The aim of this thesis is to show the main problems related to the application of the contingent valuation method in general and in the Czech Republic. First, the methodology of contingent valuation is described in detail. Since the case study on Macha Lake deals with the valuation of improvement of eutrophicated water body, the main issues regarding the water quality are discussed with a special focus on the problem of euthrophication. Following part summarizes selected attitudes towards the application of contingent valuation method on freshwater quality in foreign countries. It focuses on studies that value similar hypothetical product as the survey on Macha Lake. Main differences are identified in the attitudes towards the definition of the good to be valued. Based on this summary the survey on Macha Lake is assessed critically. The aim of this survey is to estimate the benefits that the improvement of water quality on Macha Lake presents for the local households. This change is defined as an improvement in water quality by one and by two classes on five-class water quality scale. The structure and organisation of the survey is introduced as well as its parts, outlining the solution of...
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Diversity of anaerobic ciliates
Nováková, Ludmila ; Čepička, Ivan (advisor) ; Kostka, Martin (referee)
Ciliates (Ciliata) are single-celled eukaryotic organisms belonging to the large group Alveolata. Ciliates are classified to eleven classes. Anaerobic/microaerophilic representatives belong to classes Armophorea, Litostomatea, Plagiopylea, Oligohymenophorea, and Prostomatea. The mitochondrion of the anaerobic ciliates has been tranformed to hydrogenosome. The anaerobic ciliates are characteristic for the presence of methanogenic symbionts in the cytoplasm near hydrogenosomes. Anaerobic flagellates are free-living or they live as commensals, mutualists or parasites in the digestive tract of animals including humans. The true diversity of anaerobic ciliates is still not fully understood. The reason is that only sequences of described ciliate species are usually included into phylogenetic analyses. However, many environmental sequences representing considerable part of known molecular diversity of ciliates have been published as well. The aim of this work was to obtain and analyze sequence data of anaerobic free-living ciliates. We have determined SSU rDNA sequences of 32 different freshwater and marine strains of ciliates. Phylogenetic analyses showed that the strains belonged mostly to the classes Armophorea, Plagiopylea and Oligohymenophorea. The strain LIVADIAN belonged, together with genera...
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