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The role of the nurse in transrectal prostate biopsy
HESOUNOVÁ, Dominika
The topic of this bachelor thesis is: The role of nurses in the transrectal prostate biopsy. The thesis is divided into the theoretical and the practical parts. The theoretical part focuses on the description of the prostate carcinoma, which is the second most frequent malignity in men. It describes the risk factors of the illness that may have influence on the development of prostate carcinoma, and the symptoms that the illness manifests itself by. Further, it describes the fundamental diagnostic methods and prevention measures. Of the diagnostic methods, a more detailed analysis is made of transrectal ultrasonography connected with prostate biopsy. The theoretical part also describes the nursing care of patients who undergo transrectal prostate biopsy. The nurse is an integral part of the care of these patients, not only before and during the performance but also after it.
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Preparation, expression and characterization of mouse GCPIII
Bäumlová, Adriana ; Šebo, Peter (referee) ; Konvalinka, Jan (advisor)
English abstract Glutamate carboxypeptidase II (GCPII, EC 3.4.17.21) is a type II transmembrane glycoprotein which has been discovered in nervous system as an enzyme responsible for the hydrolysis of neuropeptide N-acetyl-L-aspartyl-L-glutamate to N-acetyl-L-aspartate and L-glutamate and that has been hypothesized to influence glutamatergic signaling processes. Except for brain, GCPII was mainly found in prostate, kidney, and small intestine. In small intestine, GCPII cleaves terminal glutamates from polyglutamylated folates facilitating thus absorption of dietary folates. In prostate, this enzyme is known as prostate-specific membrane antigen and is used as a cancer marker. Mus musculus is an important model for studing GCPII and its homologs as a therapeutic target. While human GCPII and its paralog GCPIII are relatively well characterized, no biochemical study of their mouse orthologs is available. That is why mouse glutamate carboxypeptidase III (mGCPIII) was cloned, prepared by recombinant expression in insect cells and characterized. We show that pure mouse GCPIII possesses α-glutamate carboxypeptidase activity which is effectively inhibited by specific inhibitor GCPII, 2-PMPA. We also analyzed sensitivity and specifity of monoclonal antibodies against mouse GCPIII. Immunoblots demonstrate that...
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Natural drugs in cancer treatment and their cytotoxicity
Hájková, Tereza ; Raudenská, Martina (referee) ; Provazník, Ivo (advisor)
The thesis deals with the natural substances in context with the cancer disease. The natural substances have a positive effect on the human organism and they are able to influence the viability and the growth of the cancer cells. The main mechanical device is to influence the mechanisms needed to start the apoptosis of the cancer cells and stopping further proliferation. The cancer cell lines utilization in the cancer disease is discussed in the thesis too. The thesis states common methods of determining the natural substances cytotoxicity. For the experimental part of the thesis it was chosen the MTT test method and the xCELLigence system for monitoring in real time. The mechanical device of the tested substance capsaicin in application on the prostate cell lines, tumorous PC3 and nontumorous PNT1A influence will be observed within the experimental part of the thesis.
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On-chip and capillary electrophoresis for cancer research
Hájková, Tereza ; Babula, Petr (referee) ; Provazník, Ivo (advisor)
The thesis deals with cancer disease and its genesis. The prostate cancer is the second most dangerous disease in male population. It is very important to detect it in early stage. This thesis deals with the proteins which are or could be used as potential cancer markers. For example in current commonly used the prostate-specific antigen and newly the metallothionein. Metallothionein is a protein occuring in fauna and flora in four basic isoforms. There is a hypothesis that the isoform content or/and their ratio may have a diagnostic implication and could be used in clinical practise. For this reason they are studied and developed methods for specific and sensitive analysis of individual isoforms. In this work, capillary electrophoresis is used for the separation of the isoforms. The influence of the parameters such as type of background electrolyte, its pH and separation voltage etc. on the final separation are discussed.
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The role of a nurse at general practitioner's office for children and adolescents in connection to prevention of testicular cancer
NESPALOVÁ, Jana
Theory background Testes are two male reproductive glands located in the scrotum and separated protective sheath of dense connective tissue. They play major role in spermatogenesis and hormone production. They also represent secondary gender symbols and therefore are important for the personality forming of young individuals. Testicular cancer stand for about 1- 1.8% of all malignant tumour diseases in males and occur most often between 15- 35 years of age. This bachelor's work gives information on the anatomy and physiology of testes, the risk factors and causes for the occurrence of testicular cancer, and also about the symptoms and diagnostics. This thesis also deals with the role the nurse has in general practitioner's office for children and adolescents regarding the prevention of testicular cancer, preventive check-ups and self-examination. Work aim First aim of this work is to map the role of the nurse in general practitioner's office for children and adolescents regarding the prevention of testicular cancer. The research question posed was: What is the role of the nurse in general practitioner's office for children and adolescents regarding the prevention of testicular cancer? Second aim was to find out about the awareness on testicular cancer in boys 15-19. First hypothesis says: Awareness about the prevention of testicular cancer varies with age. Second hypothesis: Self-examination of testes changes with age. Used methods Qualitative research was used for the first part of the assignment, while a questionnaire and method of deep dialogues with the nurses working at general practitioner office for children and adolescents in South Bohemia was conducted for the second part. The dialogs were recorded on a dictaphone and then transcribed word for word. In the second part quantitative research was used through a method of questioning and technique of non-standardised questionnaire. The questionnaire was filled in by boys aged 15-19 living in South Bohemia. The parameter test Anova, a type of scattering analysis, was used on the data. "Its basis lies in the total scattering of the followed variable being divided into scattering within individual selections and scattering between them. If the scattering between the selections is improbably large it points towards significant influence of factor a (Budíková, 2010, s. 181)." Results The transcribed dialogs were analysed by open coding, by the pen and paper method. Five categories were defined: Education, testicular cancer, testes examination, patient dispensary. Subcategories with major data encoded, were assigned to each category. Analysis of the non-standardised questionnaire gave rise to ten resulting graphs with respondent answers. The graphs were consequently described. First hypothesis saying that the knowledge about the prevention of testicular cancer changes with age was not proven. The second hypothesis saying that the process of self-examination changes with age was not proven either. Conclusions The first research part proves that nurses working in general practitioner office for children and adolescents do educate the patient on this subject. The respondent possessed basic information on the subject of prostatic cancer and stated that testes examination is conducted in the surgeries. Scientific literature for this subject was recommended by only two of nine respondents. The respondents stated that testes examination and education about the subject of testicular cancer is conducted, although the second part of the research showed that the boys are almost not at all familiar with the subject. Furthermore, testicular examination was not conducted for most of them at the practitioner's office. The second part of research showed that the boys aged 15-19 let possess insufficient information on the prevention of testicular cancer. Most had almost no knowledge on what is involved in such prevention.
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Risk factor's of prostate cancer
Plecháčková, Anna ; Lukeš, Martin (advisor)
Even though etiology of prostate carcinoma is still unknown, its occurrence is affected by many risk factors. The most important ones being: age, race, and familiar occurrence. Also dietary factors, especially increase intake of fats and dairy products, play its role. The role of other risk factors such as androgen influence, vasectomy, sexual activity and professional exposures to kadmium remains unclear. The knowledge of these risk factors is vital for prevention of prostate cancer.
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Participation of Selected Carbonyl Reductases in Deactivation of Anticancer Drugs
Odiana, Romana ; Wsól, Vladimír (advisor) ; Szotáková, Barbora (referee) ; Heidingsfeld, Olga (referee)
Reduction is the reverse of oxidation and therefore it can involve loss of oxygen atom or the addition of two hydrogen atoms. The reduction of carbonyl groups in xenobiotics was the main topic of this thesis. We tried to identify and characterize human carbonyl reductases responsible for anticancer drugs deactivation. When cancer is among the most common death causes in the developed world, it is necessary to look for new and efficient ways of its treatment. Inhibition of enzymes, which may contribute to disease development or relapses and/or treatment efficacy decrease by drug inactivation, could be a possible way of treatment improvement and might also lead to decrease of drug doses and side effects of cytostatics. In the first part of our project, we focused on a soluble cytosolic reductase AKR1C3. This enzyme is involved in sex hormone metabolism and might play an important role in breast and prostate cancer development. We tested its ability to metabolize anticancer drugs by its incubation with oracin and doxorubicin with subsequent metabolite determination with use of HPLC. Our experiment proved that it can deactivate these two drugs with Km 355 μM for doxorubicin and 110 μM for oracin, respectively. AKR1C3 can therefore influence the anticancer therapy, expecially when overexpressed. The...
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The Effect of Initial PSA on Prognosis in Pacients Who Suffers from Prostate Cancer
Foučková, Hana ; Kindlová, Eva (advisor)
The aim is to verify whether the input level of PSA may have someinfluence on the prognosis of patients with prostate cancer. I have focused on the influence of IPSA in thefive-year survival of these patients, which is one of the relatively common internationally recognized criteria in trials .. Furthermore, I wonder if the IPSA may also reflect on otherprognostic markers Prostate cancer - if with higher histological grade, or high clinical IPSA stage level change in some way.
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