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Design and synthesis of specific colevelin derivatives for in vitro and in vivo studies
Kostomoiri, M. ; Zikos, C. ; Benaki, D. ; Slaninová, Jiřina ; Pirmettis, I. ; Papadopoulos, M. ; Pelecanou, M. ; Livaniou, E.
Colivelin is the most potent member of the humanin family of neuroprotective peptides with in vitro and in vivo action against insults associated with Alzeimer’s Disease. The mechanism of action of colivelin is not yet elucidated. The objective of the present study is the design and synthesis of specific colivelin derivatives that can be used as molecular probes in in vitro and in vivo biological experiments. In the frame of the study three colivelin derivatives bearing suitable tags, i.e the fluorescent moiety FITC, the 99mTc-radiometal chelating unit dimethylGly-Ser-Cys, and the streptavidin-counterpart biotinyl-group, were designed and synthesized. These derivatives will be used in in vitro cell component binding and in vivo biodistribution experiments, in order to gain better insight on the mode of the neuroprotective action of colivelin.
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Analysis of selected challenges when exporting fast-moving consumer goods from Mexico and suggestion of particular optimization
Kudelová, Jarmila ; Kolář, Petr (advisor) ; Staroba, Jaromír (referee)
This Master´s Thesis deals with the exportation of fast-moving consumer goods from Mexico. The theoretical part explains the concept of FMCG and describes the steps that should generally be followed when exporting FMCG from Mexico. Furthermore, the elements related to maritime transportation are described, because this type of transport of exporting from this country is used most often. Within the specific case of Corona, detailed logistics structure of the export process is displayed. By analysing its operation, the main challenges associated with carriers used for the export are identified and ultimately partial solutions that should be implemented to minimize the occurrence of problematic points are suggested.
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Izotopicky značené peptidy poskytující rozlišená strukturní data pomocí infračervených specter. Strukturní limity optické spektroskopie
Keiderling, T. A. ; Huang, R. ; Kubelka, J. ; Bouř, Petr ; Setnička, V. ; Hammer, R. P. ; Etienne, M. A. ; Silva, R. A. G. D. ; Decatur, S. M.
Isotopic labeling, here exemplified by 13C substitution on the amide C=O group, can allow site-specific determination of peptide conformation with IR. Examples of helix and sheet (beta-hairpin) structures are reviewed.
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Optimization of internal material flow
Kovář, Miroslav ; Veverka, Petr (referee) ; Simeonov, Simeon (advisor)
This thesis refers about the optimalization of internal business logistics system. This thesis want to explore and propose new solutions to the system of internal transport of material in a manufacturing company. The aim is find a suitable methodology for the problem and aplication this methodology to the particular case. The practical part describes the specific case of the production system in the Bosch Diesel s.r.o. at his plant in Jihlava. Description includes an overview of the current state of the logistical arrangements and problems that a rise from the current state. Compares the current state with the proposed state and investigates the various pros and cons. Part of the analysis is a computer simulation of the transport system, which has been support to confirming the appropriateness of the proposed solutions.
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Using DEA models to assess efficiency of public transport
Hanousek, Jakub ; Borovička, Adam (advisor) ; Soldatyuk, Nataliya (referee)
This thesis deals with using DEA models to assess efficiency of the bus public transport in the Czech Republic regions. It is a method based on linear programming used to measuring efficiency of units. The main problem of public transport is the constant pressure to increase subsidies into this sector whilst the efficiency decreases. In the theoretical part of thesis, there will be explained the term 'efficiency' and will be introduced the basic principles of data envelopment analysis method including the mathematical formulations of the classic and modified DEA models, which will be then used in the practical part. The analysis of the resulting efficiency rates of all the different regions will be carried out with respect to the regions' specific characteristics. In the conclusion the optimal values will be proposed.
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Sperm protein profiles of different mammalian species
Pohlová, Alžběta ; Zigo, Michal ; Jonáková, Věra ; Postlerová, Pavla
Proteins are a substantial equipment of the spermatic cell; therefore, the characterization of sperm proteins is crucial for explanation of molecular mechanisms in the reproduction process. We isolated sperm proteins from different mammalian species - pig, bull, human, mouse, dog and cat. Extracted proteins were separated by SDS-electrophoresis and protein/glycoprotein profiles from epididymal or ejaculated sperm were compared. Additionally, we tested cross-reactivity of antibodies prepared to sperm boar proteins on spermatozoa of other mammalian species using immunofluorescent technique. Our future plan is to compare the protein profiles of sperm during their functional development (epididymal, ejaculated, capacitated) in various mammalian species and identify species-specific sperm proteins with zona pellucida binding activity.
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Campus TRIANGL Uherske Hradiste - construction technology project
Chamrádová, Klára ; Kopecký, Lukáš (referee) ; Biely, Boris (advisor)
The subject of this thesis is a construction technology project Areal triangle in the town of. The work includes a technical report on the structural and technological project technical report construction site along with drawings of building equipment, technological prescription for the implementation process of monolithic structures, being associated inspection and test plan, as well as draft mechanical assemblies, technical report broader transport relations, including drawings, itemized budget calculation according THU and the timetable for construction. It also dealt with contract work, instructions for use of the building and assess two variants of cranes in economic terms. Also at work are chapters dealing with safety at work and environmental protection. More specifically, the work deals with one of the main buildings, namely the object SO10 - Building A. The construction is a reinforced concrete monolith on five floors. The work is mainly focused on the shell construction.
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Total contents and speciation of arsenic and selenium in plants growing in soils with different physico-chemical properties
Tremlová, Jana ; Száková, Jiřina (advisor) ; Vymazal, Jan (referee)
This dissertation thesis deals with uptake, accumulation and transformation of arsenic and selenium in plants growing on soils with different chemico-physical soil properties. The contents of arsenic were investigated in 99 wild plant species, coming from 27 different families and 6 different vegetables growing in arsenic contaminated soils. The results suggest that there are species-specific mechanisms which protects certain plants from the excessive uptake of arsenic from the soil. On the other hand, there are plants using a strategy of accumulation, which theoretically may increase the risk of arsenic entry into the food chain, whether through grazing cattle and wildlife or through consumption of vegetables growing on arsenic contaminated soils. The most prevalent arsenic compounds are AsV and AsIII. Other arsenic compounds occure as minor species. An important finding was detection of arsenobetaine in Plantago lanceolata L. and Carex praecox Schreb ..
The contents of selenium were examined in 73 species of wild plants, coming from 29 different families. The results suggest that naturally low levels of selenium in the soil of selected locations along with a low capability of selenium uptake via collected plant species lead to the low content of selenium in plant biomass which may contribute to selenium deficient throughout the food chain. Foliar application of selenate on wild plant communities and on some types of vegetables in our case Brassica oleracea var. italica can have a positive impact on increasing the selenium content in the aboveground biomass of these plants and by extension, increase the selenium content in the human diet. Dominant selenium compounds in the aboveground biomass of the analyzed plants were SeVI and selenomethionine, with variable proportion of other commonly occurring organic selenium compounds, which is mainly affected by plant species.
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