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Experimental Investigation of The Flow Pattern Behind Cylinder
Vítkovičová, R. ; Skála, Vladislav ; Čížek, J.
Investigation of the flow behind bluff bodies, especially for cylinder, is one of the highly topical research topics in the fluid mechanics. Nevertheless, mainly because of its complexity, this topic is still an open problem. This article discusses the running project at the Czech Technical University in Prague, Faculty of Mechanical Engineering and it deal with the implementation of a methodology for measuring the cylinder wake in a wind tunnel and also recapitulates the partial results obtained measurements.

Supervision in psychiatric nursing
RŮŽIČKOVÁ NEUŽILOVÁ, Michaela
Basic theoretical background The theoretical part of the thesis explores the issue of supervision and focuses on psychiatric nursing. It is divided into several parts. The first part is focused on supervision, briefly characterizes supervision as such, then it describes its divisions, functions and methods. It also explores the participants of supervision, the agreement on supervision, transcultural supervision and Balint groups. The following part describes historical development of psychiatric nursing, briefly presents the personality of Joyce E. Travelbee. In the conclusion of the theoretical part of the thesis the problems of education and competence of nurses in psychiatric care are introduced, marginally mentioning the Turku declaration. The aim of the work The main aim of the thesis is to investigate the awareness of nurses working in psychiatric care of the clinical supervision options. The task of the second objective is to find out the opinion on supervision of nurses working in psychiatric care. The third objective is focused on means to assist nurses working in psychiatric care in their personal development and growth within their practices. The purpose of the fourth objective is to find experience with supervision of nurses working in psychiatric care. The methods used Qualitative investigation was chosen for the empirical part of the thesis. The method used for data collection was in the form of semi-structured interview. The interviews with the respondents were taken down using a voice recorder and transcribed, qualitative data was then analyzed by pencil and paper encoding technique and semantically organized into 27. groups of categorization. The selection of a research sample was intentional, consisting of twelve nurses working in psychiatric care in the Czech Republic. Results The research focused on nurses who do not have personal experience with supervision revealed that they have certain awareness of the supervisory process, however not quite sufficient to be able to describe the nature and function of supervision in its entirety. Their knowledge is rather theoretical. They understood supervision as help to solve disharmonious relationships in the workplace, as well as it could improve communication skills of the nurses, it would also assist in dealing with crisis intervention and prevent burnout syndrome. The results regarding nurses who do not participate in the supervisory process clearly indicated that they have insufficient support and the opportunity for both their professional and personal growth in their workplace, while this important could be solved by supervision. The research, which covered only nurses who have had personal experience with supervision and regularly participate in it, showed that their knowledge and skills related to the supervision process are quite sufficient to be able to understand and use supervision practically in the whole range. These nurses sees supervision as a benefit that would allow them not only to understood themselves, but also to learn how to manage challenging situations that are a part of their profession. Supervision gives them confidence, provides support and teaches them to deal with people who are part of their work process. Supervision has become a tool for preventing burnout and showed them how to use their own potential effectively. Conclusion This thesis is focused on the use of supervision in psychiatric nursing. Working with mentally challenged patients is very demanding, therefore supervision plays its justified role there. If you do not know yourself and you are not able to solve your own problems, you can never effectively and comprehensively address the problems of others, who are often desperately waiting for your help. The results will be presented at professional seminars designed not only to nurses working in psychiatric care.

The need for security and safety in connection with a planned surgery with the patient's perspective and nurses
BOHDALOVÁ, Jaroslava
A planned operation performance always affects patient´s needs for assurance and safety. It is important to provide necessary information connected with the planned operation performance itself so that the patient can feel assure and safe. The key role for the patient and her/his needs for assurance and safety is in general nurse´s hands because she is the closest person to the patient and can help to inspire her/his needs for assurance and safety with konfidence best through the aid of her knowledge and skills that she should have as well as through the aid of her attitudes. The target of the thesis was to find out how a planned operation performance affects patient´s needs for assurance and safety and how a general nurse inspires patient´s needs for assurance and safety connected with a planned operation performance with confidence. The following research questions were stated: How does a planned operation performance affect patient´s needs for assurance and safety? How does a general nurse affect patient´s needs for assurance and safety connected with a planned operation performance to inspire her/him with confidence? What is insufficient in filling patient´s needs for assurance and safety before a planned operation performance? How can a general nurse help a patient control her/his fear and state of anxiety related to a planned operation performance? The method qualitative research with the use of depth interview was used to figure out the investigation. The respondents were willing to record the interview which were verbally rewritten and then subjected to the contents analysis by the method paper and pencil. From the research it is clear that a planned operation performance affects patient´s needs for assurance and safety in a negative way disregarding to her/his previous experience. Patient´s worries and negative feelings can be eliminated by a general nurse who treats her/him as equal, taking up an individual approach, informing her/him about all performances and proceedings, having a calming and welcoming way of communication, using empathy and listening as well as trying to meet patient´s wishes according to her possibilities. The research reveals the advantage of patients´ readiness for planned operation performances. Patients can learn it in the framework of educational proceeding in the out-patients´ department at the same time when they learn that they are going to be operated. The research also shows that general nurses do not have enough knowledge in the sphere of therapeutical communication. The results indicate that it would be suitable to start educating patients as soon as it is known how their disease will be solved and treated in order to reduce the negative impact of surgical treatment to patients´ needs for assurance and safety. General nurses should complete their knowledge and skills to be able to help patients to cope with their difficult life-situation. On the basis of the research investigation and after the consultation with the senior nursing officer, the training course will be organized. The topic of the training course will concentrate on communication with patients before planned operation performances with a view to therapeutical communications and therapeutical interviews. Other discovered facts, for example, the lack of information provided to patients and possibilities regarding better patients´ readiness for planned operation performances will surely be solved by the hospital management.

Iniciace pohybu byčíku, signalizace a regulace pohyblivosti spermií ryb: fyzikální a biochemické řízení
PROKOPCHUK, Galina
The current study attempted to shed light on the regulatory processes and response arrangements of fish spermatozoa during the course of maturation and motility initiation. The first intent of this study was to improve the understanding of the mechanism underlying the acquisition of potential for sperm motility in sturgeon. Up to present work, the physiological process underlying sperm maturation in this species has not been described at all. Our results showed that sperm maturation in sturgeon occurs outside the testes because of dilution of sperm by urine and involves the participation of high molecular weight substances as well as calcium ions present in seminal fluid and/or urine. The second aim of the present study was to investigate the coping mechanisms in fish spermatozoa with osmotic and ionic activating mode, as well as in spermatozoa of euryhaline fishes, to various environmental conditions. We showed that alteration of environmental osmolality might affect the fish sperm in different ways, depending on fish species and modes of spermatozoa motility activation either osmotic or ionic mode. In response to osmotic stress caused by hypotonicity, carp spermatozoa regulated the flow of water across their cell membrane and increased their cytoplasmic volume during their short motility period. In contrast, no indications of sperm volume changes were observed neither in sterlet nor in brook trout spermatozoa, both of which having an ionic mode of motility activation. We also examined the mechanism by which sperm motility triggering in euryhaline fishes can adapt to a broad range of environmental salinity. Our results demonstrated that spermatozoa of euryhaline tilapia, Sarotherodon melanotheron heudelotii, reared in fresh-, sea- or hypersaline water can be activated in hypotonic, isotonic or hypertonic conditions of swimming milieu, provided Ca2+ ions are present at various levels. It was established that the higher the fish rearing salinity or the more hypertonic ambient media at spermatozoa activation, the higher extracellular concentration of Ca2+ ions is required. The results obtained in the present study allow suggesting that osmolality is not the main factor inhibiting sperm motility inside the testis in the S. melanotheron heudelotii. A third aim of this study was investigation of the regulation of motility initiation process and description of flagellar beating initiation in chondrostean spermatozoa. We detected that K+ inhibition of sperm motility in sturgeon can be by-passed due to the pre-exposure of sperm cells to a high osmolality shock prior to its transfer to K+-rich swimming media. Thus, we hypothesized that sturgeon spermatozoa may be activated by use of an unexpected signaling pathway, independent from regular ionic stimulation. The successive activation steps in sturgeon spermatozoa were investigated by high-speed video microscopy, using specific experimental situation, where sperm motility initiation was delayed in time up to several seconds. At motility initiation, the first couple of bends formed at the basal region begins to propagate towards the flagellar tip, but gradually fades when reaching the mid-flagellum. This behavior repeats several times until a stage where the amplitudes of bends gradually reach similar value, what eventually leads to sperm progressive displacement. The total period needed for the flagellum to switch from immobility with rigid shape to full activity with regular propagating bends ranges from 0.4 to 1.2 seconds. In conclusion, the results of the current study bring valuable pieces of information into the general understanding of the processes of maturation of fish spermatozoa, their adaptability to different physical and biochemical circumstances, the extra- and intra-cellular signaling as well as the regulatory mechanisms of motility activation in fish spermatozoa.

Characteristics important for ensuring of sustainable and development of dairy cattle herds breeding
Lelek, Jaroslav ; Stádník, Luděk (advisor) ; Petr, Petr (referee)
The aim of this dissertation was to assess the influence of characteristics important for ensuring of sustainable and development of dairy cattle herds breeding. The data investigated describe approximately 5138 dairy cows in period of four years in selected farming´s of dairy cattle in district of Jičín. There were these indicators of reproduction processed: conception rate after first insemination, conception rate after all inseminations, interval of insemination, index of insemination, service period and meantime; the data were collected from seven stables of Bohemian Spotted cattle and from seven stables of Holstein cattle. The longevity was investigated from the number of dairy cows at respective lactation of both investigated breeds and from the average lactation in respective years. The results of investigation prove that the average percentage representation of dairy cow at respective lactation between 2012-2015 ranged 33,4 % at first lactation, and 24,68 % at second lactation, and 18,13 % at third lactation, and 11,48 % at fourth lactation, and 6,53 % at fifth lactation, and 3,32 % at sixth lactation, and 2,36 % at seventh and more lactations. The evident data from comparison prove, that till third lactation there is higher percentage representation of Holstein cattle and from third lactation there are more cows of Bohemian Spotted cattle, and also the average reached lactation confirms this the value of 2,75 lactation for Bohemian Spotted cattle and the value of 2,3 lactation for Holstein cattle. The influence of breed on the average length of lactation was quite weak. The results of rating indicators of reproduction also have not proven any statistically important difference between both types of cattle as concerns values reached by each cattle, but the original Bohemian Spotted cattle reached better results in all indicators, and thus it is probably more suitable for the area of district Jičín.

Family and reading fairy story
NĚMCOVÁ, Petra
This thesis deals with reading fairy tales in families. Theoretical part acquaints with the parental position, also with the attitudes of fairy tales and readership, with reader´s literacy and its development. From the psychological point of view it is dedicated to the influence of fairy tales on child, their indispensability in the pre-school period, and the topic of rituals. In the practical part mixed research results are presented, by means of that the attitude of parents on fairy tales and readership has been determined. Whether sense of reading fairy tales for children, for their development, orientation in emotional experience and understanding of life journey is perceived. Furthermore, it was investigated whether fairy tales appeared as a ritual before bedtime, their overall frequency and timing. What influences affect on this issue related to reading fairy tales in families. What parents themselves consider as a ritual and what additional evening rituals occur in families.

Sandstones and conglomerates Peruc Mbrs (lower Cenomanian , Upper Cretaceous ) of eastern Bohemia and coal mass in them
Martinec, Petr ; Kubina, Lukáš ; Rojáková, T.
A reasonably complete knowledge of the development of East Bohemia’s Lower Cenomanian sediments (Peruc Mbr) is still lacking. The sediments originate from rivers flowing from the buried Hořice-Nové Město elevation to Moravia in the SE direction, via a flatland depression. The sandstone and conglomerate bodies within the Litice Chlum area under investigation are channel river sediments accompanied by sedimentation in river lakes or oxbow lakes. The landscape of the region showed the character of a low-relief peneplain. At Sopotnice site, the surface of Litice kaolinite massif of granodiorite with a weathered crust incorporates fine-grained conglomerates with white quartz and quartzite pebbles in a sandy matrix. They form wedge-shaped channel bodies of several dozens centimeters thickness. The textures are massive, with inconspicuous positive gradation bedding of medium to poor bed sorting. By their character they correspond to the fill of an instable riverbed that kept changing its position in the flatlands. In the matrix, kaolinite dominates over illite.
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Biotransfer of selected risk metals into plants and their accumulation and distribution in plant organs
Le Minh, Phuong ; Lachman, Jaromír (advisor)
Contamination of soils with heavy metals is one of the serious environmental problems threatening human being. Heavy metals are considered as the special hazard of soil pollutants because of the adverse effects on the plant growth, the amount, activity of useful microorganisms in soils and the quality of food. Regard to the persistent and toxicity, the heavy metals are toxic when we consider different kinds of pollutants in soils. In the soil, zinc (Zn), cadmium (Cd), lead (Pb) and mercury (Hg) toxicities frequently occur than the other metals because of their precipitation and sorption by the soil. It is a very dangerous situation because when these metals are taken up by plants, they can be transported to the food web and food chains. In the present study, the accumulation of four heavy metals (mercury, zinc, lead and cadmium) in the whole grain of spring accessions of emmer, einkorn and common spring wheat cultivars and potato (Solanum tuberosum) is reported. Heavy and essential elements were monitored in potato cultivars in the exact field experiments and in hydroponically grown plants. The elements were determined by methods FAAS, ET AAS, and AMA (Advance Mercury Analysis). Statistical analyses were performed using SPSS 9.0 with the Tukey HSD (Honestly Significant Difference) test (alpha equal to 0.05). In our study, the concentration of heavy metals decreased in the order zinc (Zn) > lead (Pb) > cadmium (Cd) > mercury (Hg) in the wheat grain. The comparison between three varieties of investigated wheat revealed that the emmer wheat was rich in zinc content (62.12 mg kg-1 dry matter), while the spring wheat had the lowest average concentration of zinc in the grain (40.99 mg kg-1 dry matter). Generally, the values of lead concentration in grain wheat varieties were low (ranging from 0.1268 mg kg-1 dry matter to 0.2950 mg kg-1 dry matter). The concentrations of mercury in four typical growth stages of wheat (boot stage 10, heading stage 10.2 1/4 of head emerged, leaf-stage 10.2 and stage ripening 11 according to Feekes) were also determined. It has been shown that the concentrations of mercury in different wheat varieties were absorbed differently at different growth stages of plant. Stage 10.2 and leaf stage 10.2 showed the high mercury content (0.0152 mg kg-1 dry matter and 0.0214 mg kg-1 dry matter, respectively). Among individual varieties significant differences were determined. Amounts of toxic and potentially toxic elements detected in investigated potato tubers are characterized by a large variability within investigated groups. Performing statistical analysis (one way ANOVA) showed that there were no significant differences between two investigated groups of samples (samples from Uhříněves and Valečov in the year 2013 and 2014) considering either one of investigated metals. Measurable levels of mercury were found in smallest amounts in all investigated potato samples comparing to other metals (Cd, Pb). Plant cells compared to animal cells are characterized by the formation of cell walls. Plasma membrane or cell membrane is a biological active membrane separating the interior of cell from the outside environment. An adjusted method for isolation of protoplasts was developed and adapted for isolation of protoplasts from plant material (potatoes). In our experiment, the plants were grown hydroponically in the Research Institute of Plant Crops Prague-Ruzyně. If we examine the plant membrane, one option is to remove the cell wall by means of special mixture enzymes. Protoplasts were released in the dark at 25 degrees of Celsius for 18 hours. The 70 and 90 microns sieve was used to filter and then centrifugation for 5 minutes at 100 x g. All the steps were carefully carried out to prevent the damage or breakage of protoplasts.

Towards a better understanding of ungulate diets: a methodological approach
Holá, Michaela ; Červený, Jaroslav (advisor) ; Stádník, Luděk (referee)
Populations of European ungulates have grown substantially over recent decades, resulting in considerable environmental and socio-economic impacts. Availability and quality of natural and supplemental food sources are among the main factors driving their population dynamics. Detailed knowledge of feeding strategies of management-targeted species is therefore of primary importance for their successful management. Over time, methods to study the feeding strategies of animals have also evolved considerably but each has its advantages as well as limitations. This doctoral thesis uses a combination of traditional methods (i.e. stomach content analysis) and novel methods (i.e. stable isotope analysis, near infrared reflectance spectroscopy) to achieve a better understanding of feeding strategies of two important ungulate species (i.e. wild boar and red deer) in the Czech Republic, where their populations are on the rise and supplementary feeding is rampant. Next, this dissertation aims to introduce and to establish a basis for these novel methodological approaches for the study of free-ranging individuals. In this respect, it also addresses methodological issues related to their application in this field. The diet composition of wild boar was investigated by examining stomach contents in order to identify their dependence on food resources of human origin (i.e. agricultural crops and supplemental foods). Foods of human origin were the dominant diet type and constituted the bulk of the diet of wild boar throughout the year. A necessary prerequisite for application of stable isotope analysis is the trophic discrimination factor (i.e. systematic difference between the isotopic composition of the consumer tissues and that of the diet), which was experimentally determined for stable carbon and nitrogen isotopes, the two most commonly used in ecology, in hair tissue of wild boar and red deer. Furthermore, possible sources of variation (such as sex, age, body weight, and lactation) on isotopic discrimination were investigated. The results of the experiments provide a starting point for the successful use of stable isotope analysis in field studies on wild boar and red deer. Finally, application of near infrared reflectance spectroscopy was proven to be useful in measuring faecal indices of food quality for red deer. The results of this thesis will contribute towards efficient management of wild boar and red deer in the Czech Republic.

Cloud computing technology framework and reducing risks
Akrir, Khaled Ali Ahmed ; Havlíček, Zdeněk (advisor) ; Macák, Tomáš (referee)
The thesis investigates, in a qualitative way, the vectors that contribute to cloud computing risks in the areas of security, business, and compliance. The focus of this research is on the identification of risk vectors that affect cloud computing and the creation of a framework that can help IT managers in their cloud adoption process. Economic pressures on businesses are creating a demand for an alternative delivery of the model that can provide flexible payments, dramatic cuts in capital investment, and reductions in operational cost. Cloud computing is positioned to take advantage of these economic pressures with low cost IT services and a flexible payment model, but at what risk to the business? Security concerns about cloud computing are heightened and fueled by misconceptions related to security and compliance risks. Unfortunately, these security concerns are seldom, expressed quantifiably. To bring clarity to cloud computing security, compliance, and business risks, this research focuses on a qualitative analysis of risk vectors drawn from one-on-one interviews with top IT experts selected. The qualitative aspect of this research separates facts from unfounded suspicions, and creates a framework that can help align perceived risks of cloud computing with actual risks. The qualitative research was done through interviews with experts and through the survey to measure risk perceptions about cloud computing using a Likert scale. The decision-making model and the framework created by this research help to rationalize the risk vectors on cloud environments and recommend reducing strategies to bring the IT industry one step closer to a clearer understanding of the risks-tradeoffs implications of cloud computing environments.