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Modelling, parameter estimation, optimisation and control of transport and reaction processes in bioreactors.
ŠTUMBAUER, Václav
With the significant potential of microalgae as a major biofuel source of the future, a considerable scientific attention is attracted towards the field of biotechnology and bioprocess engineering. Nevertheless the current photobioreactor (PBR) design methods are still too empirical. With this work I would like to promote the idea of designing a production system, such as a PBR, completely \emph{in silico}, thus allowing for the in silico optimization and optimal control determination. The thesis deals with the PBR modeling and simulation. It addresses two crucial issues in the current state-of-the-art PBR modeling. The first issue relevant to the deficiency of the currently available models - the incorrect or insufficient treatment of either the transport process modeling, the reaction modeling or the coupling between these two models. A correct treatment of both the transport and the reaction phenomena is proposed in the thesis - in the form of a unified modeling framework consisting of three interconnected parts - (i) the state system, (ii) the fluid-dynamic model and (iii) optimal control determination. The proposed model structure allows prediction of the PBR performance with respect to the modelled PBR size, geometry, operating conditions or a particular microalgae strain. The proposed unified modeling approach is applied to the case of the Couette-Taylor photobioreactor (CTBR) where it is used for the optimal control solution. The PBR represents a complex multiscale problem and especially in the case of the production scale systems, the associated computational costs are paramount. This is the second crucial issue addressed in the thesis. With respect to the computational complexity, the fluid dynamics simulation is the most costly part of the PBR simulation. To model the fluid flow with the classical CFD (Computational Fluid Dynamics) methods inside a production scale PBR leads to an enormous grid size. This usually requires a parallel implementation of the solver but in the parallelization of the classical methods lies another relevant issue - that of the amount of data the individual nodes must interchange with each other. The thesis addresses the performance relevant issues by proposing and evaluation alternative approaches to the fluid flow simulation. These approaches are more suitable to the parallel implementation than the classical methods because of their rather local character in comparison to the classical methods - namely the Lattice Boltzmann Method (LBM) for fluid flow, which is the primary focus of the thesis in this regard and alternatively also the discrete random walk based method (DRW). As the outcome of the thesis I have developed and validated a new Lagrangian general modeling approach to the transport and reaction processes in PBR - a framework based on the Lattice Boltzmann method (LBM) and the model of the Photosynthetic Factory (PSF) that models correctly the transport and reaction processes and their coupling. Further I have implemented a software prototype based on the proposed modeling approach and validated this prototype on the case of the Coutte-Taylor PBR. I have also demonstrated that the modeling approach has a significant potential from the computational costs point of view by implementing and validating the software prototype on the parallel architecture of CUDA (Compute Unified Device Architecture). The current parallel implementation is approximately 20 times faster than the unparallized one and decreases thus significantly the iteration cycle of the PBR design process.
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Plant microclimate control
Jelínek, Jan ; Neděla, René (advisor) ; Papežová, Stanislava (referee)
This bachelor thesis discusses the management of a microclimate environment inside an
enclosed box. The aim of the work is to propose and implement a program for the
automized control of the microclimate. Microclimate management is ensured by a PLC
(Programmable Logic Controller) system utilizing the program Mosaic. Radishes
(Raphanous sativus L.) were chosen as the test plant. Optimal microclimate conditions for
the chosen test plant were achieved by programming the management system. The system
is easily adaptable, meaning endless different species of plants can be grown properly.
Healthy plant growth indicates the correct parameters of the microclimate.
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Logistic Controlling And Its Role In Business Management
Šimáně, David ; Štůsek, Jaromír (advisor) ; Lenka, Lenka (referee)
The Master´s thesis focus on evaluation of logistics controlling as a support of logistics process in Škoda Auto a.s. and suggests a solution to company´s logistics controlling case study. Firstly the thesis defines theoretical basics such as enterprise management, controlling and its role in company management, tasks of controller or logistician and specifics of logistics controlling. Then it includes the explanation of Make or Buy method which is used in solving the case study. The practical part analyzes particularities of logistics
and its controlling in Škoda Auto. It specifies the main tasks of controlling department of the company especially planning, controlling, analysis of variances, tracking and optimization costs, reporting for the logistics management and other departments. At the end of this section the case study is presented and solved, which is an illustration optimization of logistics costs, which provides logistics controlling. It evaluates practical benefits of logistics controlling within the company´s logistics process and proposes recommendations to increase the efficiency of logistics controlling weaknesses.
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An evaluation of erosion risks and design of erosion control measures in selected cadastral area
Janota, Petr ; Janků, Jaroslava (advisor) ; Karel, Karel (referee)
Erosion is exogenous geomorphological process that affects the formation of the Earth's surface since the formation of the Earth's solid crust. This activity, which under natural conditions proceeded slowly, in terms of human generations imperceptibly, in intensively used landscape dramatically accelerated and brought a number of adverse consequences. The aim of this study has been to assess and evaluate erosion risks in selected cadastral area and in the event of an over limit erosion hazard to suggest appropriate erosion control measures to eliminate the increased erosion.
The 77 erosion of closed units were examined by a computer program Atlas DMT erosion module, which uses digital terrain model together with data from databases or BPEJ or LPIS. The 14 of them have diagnosed overlimit value wash away the soil. As a basic erosion control measures the change of applied classic crop rotation to crop rotations using soil conservation technologies was considered. After adjusting cropping practices that positively impact factor of the protective effects of vegetation, it was found by erosion Atlas module, six parcels of land with over limited value of soil washes. These lands have suggested the use of technical erosion control measures, for example furrows, grassing thalwegs etc.. On the parcels, where, due to their size, shape or morphology technical measures proved inadequate or ineffective it has been proposed permanent grassing.
In the proposals erosion control measures it is necessary to combine the maximum efficiency of measures with condition of ease and minimal restriction of land users. When their making is to be assumed towards the user, because it depends on him only whether the proposed organizational and agronomic measures will be implemented or not. The fundamental problem with these measures is that their implementation is not backed by legislation. I assume that the more acceptable, less restrictive and inexpensive measures will be proposed, the more likely it will be implemented. One of the reasons why even the simple erosion control measures are put into practice slowly and with difficulty is the fact that in the Czech Republic the most of the agricultural land is managed by entities that are not its owners. This fact significantly contributes to the fact that land is viewed merely as a means of production, which must to bring maximum profit only. To improve this situation may also contribute to the establishment and consistent control of the GAEC standards.
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Influence of term of winter rapeseed (Brassica napus) desiccation on yield and seed quality
Rajtmajer, Stanislav ; Bečka, David (advisor) ; Petr, Petr (referee)
Rapeseed is the world's third most important oil plant (the second seminal). It is the most cultivation and most important oil plant in Czech Republic. Winter rapeseed reaches about 85% of the harvest area of oilseeds in the country. The desiccation of oilseed crop is sphere of agricultural engineering, which is still worth discussing. The main problems are how to use the product, what dose and which dates to choose to desiccate. The results of this thesis could help to partially clarify this complex issue.
The aim of the thesis is to observe the effect of different terms oilseed rape desiccation by glyphosate on yield and seed quality.
Small-plot experiments to investigate the influence of the term desiccation of winter rapeseed were established in the years 2013/2014 and 2014/2015 on the lands of the experimental station of the Czech Agricultural University, Faculty of Agronomy Food and Natural Resources at the Červený Újezd.
In the first experimental year 2013/2014 was founded five variants in three replications (including undesiccated control). In the year 2014/2015 was founded seven variants in four replicates (including undesiccated controls). In the first experimental year was the first term desiccation 16. 6. 2014, the second year 8. 6. 2015. Subsequent periods of desiccation were a week apart.
The variety of winter rapeseed used for the experiments was a hybrid variety Rohan. The desiccating agent was used Dominator active substance glyphosate. The dose of
4 l / ha + 200 l H2O.
For all samples, both experimental, years yield was determined, the weight of a thousand seeds and oiliness. For desiccated variants were carried out pre-harvest analysis for the determination of solids in pods. Further, all harvested samples taken for laboratory germination test (Determination of germination) ISTA according to the methodology. The results of the experiment sprouting were statistically analyzed using ANOVA analysis of variance. Differences between mean values were evaluated by Tukey test, the computer program SAS at a significance level of p = 0.05.
Effect of desiccation term influences of winter oilseed rape seed quality in terms of weight and thousands of seeds in terms of oil content. Too early desiccation (46 to 39 days before harvest) HTS reduced by 7-17% and the oil content of 2-4% of the overall average. Oiliness of the observed characters minimum interference term desiccation. Desiccation in the optimum date (17 days before harvest) increased oiliness of 1-6%.
Influence term desiccation oilseed rape also greatly influences seed yield.
Too early desiccation (46 to 39 days before harvest) reduced the yield by 11-14%. Undesiccated control in both years achieved the highest yields, increase yield by 5-12%. Desiccation in the optimum date (17 days before harvest) increased the yield of attempts by 5-6%.
The term desiccation of winter rapeseed, also significantly affects the vitality of seeds, where very reduces energy germinating seedlings in the first days. The term desiccation, however, does not affect overall seed germination. Too early desiccation (46 to 39 days before harvest) EK2 decreased by 12-40%, EK3 decreased by 4-24%, 3-4% EK4 and extended MGT of 7-15%. In the first experimental year was the most vital option undesiccated control (EK2 = 50.4% = 91.3% EK3, EK4 = 97.9%). In the second experimental year was the most vital seeds of the optimal term desiccation, (Sixth term, 17 days before harvest) = 68.9% EK2, EK3 = 98.2%, EK4 = 100%. Seed samples of 2014/15 had a higher overall vitality of seeds, than samples from 2013/14, the overall vitality is probably worse given year old and transsilaged seed.
The results of the two-year experiment that term desiccation affects the quality of seeds, the yield of seeds and vitality of seeds. Pre-analysis was determined optimum solids content in siliques desiccated samples to values of 40-50%. Like most technology seems to desiccation in the optimum date (17 days before harvest) and agro technology without desiccated vegetation. As the least appropriate technology seems very early desiccation (46 to 30 days before harvest).
The first scientific hypothesis: Premature desiccation reduces seed yield, oil content and HTS. Yes, the hypothesis was confirmed.
The second scientific hypothesis: Desiccation made in the correct term do not affect the quality of the seeds (oiliness and HTS). Yes, the hypothesis was confirmed.
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Control of web analytics implementation
Kocourek, Tomáš ; Šimek, Pavel (advisor) ; Ladislav, Ladislav (referee)
The diploma thesis is focused on verifying the accuracy of web Analytics Adobe Analytics. Manual control is a time-consuming activity, therefore web analysts are looking for ways leading to its acceleration and automation. Selected problem was solved by writing VBA macro in MS Excel. Macro checks the reports that are uploaded into Excel from analytic database using Adobe Report Builder. The theoretical part is focused on explaining the role of web analytics in online marketing and related concepts. The practical part presents the requirements for macro in Excel and then interprets all functions and procedures created by the program. Thesis also includes the configuration of the ObservePoint system competitive environment, which will be the last part compared with created macro. Created solution in Excel allows to verify the accuracy of web analytics with clearly sorted results quickly, thereby replaces the previous manual control.
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Small terrestrial mammals as an indicator of the restoration Sokolov area after coal mining
Červená, Lucie ; Kouba, Marek (advisor) ; Markéta, Markéta (referee)
Aim of this paper was to describe the diversity of small terrestrial mammals on selected habitats of Velka podkrusnohorska spoil heap and surrounded areas. Between years 2013 2015 there was realized seven catching on the twenty seven study sites. In every year we repeated a catches in spring and autumn period. The selected habitats were: wetlands, meadows, pine forests, oak forests and successional forests. As a capture method was chose standard line method with usage of snap traps. The obtained data were statically analysed by the R program and the diversity was compared based on the Shannon-Wiener index of diversity. We compared abundance and diversity between spoil heap study sites and surrounding study sites, which were situated out of the Velka podkrusnohorska spoil heap and was sign as control sites. We chose three study sites of every habitat situated on spoil heap and another three sites of every habitat situated out of the spoil heap (control sites). In total we caught 720 small terrestrial mammals of ted species: Crocidura leucodon, Crocidura suaveolens, Microtus arvalis, Microtus agrestis, Mus domesticus, Apodemus sylvaticus, Apodemus flavicollis, Myodes glareolus, Sorex araneus and Sorex minutus.
The highest diversity was on the four wetlands sites which three of them were situated on the spoil heap. The highest abundance was at the meadow site Matyas which was situated on the spoil heap. In the overall assessment of the abundance and diversity of communities of small terrestrial mammals was confirmed equality of spoil heap sites and control sites. In the assessment of abundance and diversity of individual habitats was confirmed equality of spoil heal sites and control sites. Exception was abundance between forests spoil heap sites and control sites, where the equality of abundance was rejected because of higher abundance of control sites. We decide to compare technically restored sites and sites using managed successions on the spoil heap. The diversity of technically restored pine forests and successional forest was confirmed but the equality of abundance of these sites was rejected because of the higher abundance of successional forests. The equality of diversity and abundance of technically restored oak forests and successional forests was rejected because successional forests had higher both of these values.
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Differences in CO2 Extraction of Vegetable Oils, Essential Oils, and Minor Components from Plants
Sovová, Helena
Significant progress in understanding the phenomena that control supercritical fluid extraction from plants was made in the last years and is reflected in the literature. Combination of chemical engineering approach and botanical knowledge has appeared to be fruitful for the choice of extraction model and even for the prediction of extraction kinetics. This contribution refers to the papers utilizing knowledge of plant microstructure and location of extracted substances together with extraction equilibrium and attempts to specify the differences among extraction kinetics of vegetable oils, essential oils, and other substances. The most abundant substances like vegetable oils in seeds can be regarded in first approximation as non-interacting with plant matrix, in contrast to the minor components whose equilibrium concentration in CO2 is affected by their adsorption on matrix and often also by co-extracted substances.
Fulltext: content.csg -  PDF
Plný tet: SKMBT_C22012102414100 - PDF
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Automation of the cultivation process in term of program control of temperature and moisture
Beneš, Vojtěch ; Papežová, Stanislava (advisor) ; Neděla, René (referee)
Theoretical part of this thesis describes different kinds of sensors used for measuring temperature and humidity of air. These sensors are categorized according to the principle on which they operate. It also describes their characteristics like range, accuracy and other aspects important for determining suitability for a glasshouse automation system.
Practical part of this work covers design of this system which can be used for auto-mated controll over temperature and humidity. It reasons about suitability of different kinds of sensors and materials. It also describes a software system capable of maintaining and changing humidity and temperature based on user input. Results of different mea-surements done during the system's operation in a variable environment are evaluated to draw conclusions about the system's ability to maintain constant temperature and humidi-ty levels inside the glasshouse.
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