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The Controlling Study
Herda, Tomáš ; Mikovcová, Hana (advisor) ; Herda, Zdeněk (referee)
The main goal of this Diploma´s Thesis is to make a model for calculation of water and sewer rates for the company Vodovody a kanalizace Náchod, a.s. when sticking to the set criteria both from the side of VaK Náchod, a.s. and law regulations. Based on the theoretical part an analysis of customer sensitivity to the price changes using the data for last 20 years follows. Findings from the first two parts are used in risk analysis in next part. The created model calculates the water and sewer rates based on the information from the company accounting system in the way to generate sufficient financial resources to fulfill the renovation plan of infrastructural property plant and equipment and to transfer given amount to the company funds. In addition, the model monitors whether the legal condition of maximal allowable increase of profit per m3 is met. In the customer sensitivity to the price changes part the price elasticity of demand for water and sewer rates is calculated based on the data from 1995 to 2015. The assumption of inelastic demand is confirmed. Risk analysis part is deals with potential risk regarding the demand and prices. Potential impacts for the most significant risk are quantified. The analysis uses knowledge gained in the first two parts. It was confirmed that potential risks are exiting but do not have any significant impact on the going concern of VaK Náchod, a.s. The created model has been already used for the calculation of prices for the year 2017. Customer sensitivity analysis to the price changes and link to the potential risks is an additional information for VaK Náchod, a.s. which validates that nowadays, there are no significant threats which could affect the demand and water and sewer rates significantly.
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Modelling, parameter estimation, optimisation and control of transport and reaction processes in bioreactors.
ŠTUMBAUER, Václav
With the significant potential of microalgae as a major biofuel source of the future, a considerable scientific attention is attracted towards the field of biotechnology and bioprocess engineering. Nevertheless the current photobioreactor (PBR) design methods are still too empirical. With this work I would like to promote the idea of designing a production system, such as a PBR, completely \emph{in silico}, thus allowing for the in silico optimization and optimal control determination. The thesis deals with the PBR modeling and simulation. It addresses two crucial issues in the current state-of-the-art PBR modeling. The first issue relevant to the deficiency of the currently available models - the incorrect or insufficient treatment of either the transport process modeling, the reaction modeling or the coupling between these two models. A correct treatment of both the transport and the reaction phenomena is proposed in the thesis - in the form of a unified modeling framework consisting of three interconnected parts - (i) the state system, (ii) the fluid-dynamic model and (iii) optimal control determination. The proposed model structure allows prediction of the PBR performance with respect to the modelled PBR size, geometry, operating conditions or a particular microalgae strain. The proposed unified modeling approach is applied to the case of the Couette-Taylor photobioreactor (CTBR) where it is used for the optimal control solution. The PBR represents a complex multiscale problem and especially in the case of the production scale systems, the associated computational costs are paramount. This is the second crucial issue addressed in the thesis. With respect to the computational complexity, the fluid dynamics simulation is the most costly part of the PBR simulation. To model the fluid flow with the classical CFD (Computational Fluid Dynamics) methods inside a production scale PBR leads to an enormous grid size. This usually requires a parallel implementation of the solver but in the parallelization of the classical methods lies another relevant issue - that of the amount of data the individual nodes must interchange with each other. The thesis addresses the performance relevant issues by proposing and evaluation alternative approaches to the fluid flow simulation. These approaches are more suitable to the parallel implementation than the classical methods because of their rather local character in comparison to the classical methods - namely the Lattice Boltzmann Method (LBM) for fluid flow, which is the primary focus of the thesis in this regard and alternatively also the discrete random walk based method (DRW). As the outcome of the thesis I have developed and validated a new Lagrangian general modeling approach to the transport and reaction processes in PBR - a framework based on the Lattice Boltzmann method (LBM) and the model of the Photosynthetic Factory (PSF) that models correctly the transport and reaction processes and their coupling. Further I have implemented a software prototype based on the proposed modeling approach and validated this prototype on the case of the Coutte-Taylor PBR. I have also demonstrated that the modeling approach has a significant potential from the computational costs point of view by implementing and validating the software prototype on the parallel architecture of CUDA (Compute Unified Device Architecture). The current parallel implementation is approximately 20 times faster than the unparallized one and decreases thus significantly the iteration cycle of the PBR design process.
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Fytochemical potencial of polyfenols contain in berries
KRUMPHANZLOVÁ, Iva
This thesis sums up findings on polyphenols and their positive influence on human health. In these fytochemicals, unlike in micronutrients, recommended daily amount cannot be estimated. Micronutrient deficiency in food can cause deficiency symptoms, while in polyphenol deficiency, the deficiency does not occur. When they are present in sufficient amount in food, it is considered functional food, which is important for civilization illnesses prevention. At the beginning of the theoretical part, the polyphenol classification from pharmacological and chemical point of view is represented. On the basis of this classification important role of antioxidants and flavonoids is described. Special attention is being paid to resveratrol, which is populardue to its well known "French paradox". The following part considers the polyphenol influence on human health. The way in which polyphenols protect our body from certain diseases is explained in detail. Most of the health benefits are supported by studies conducted on animals. These studies are also mentioned and described in this paper. In the theoretical part the process of absorption and the polyphenols safety for human body is also explained. Polyphenol sources are listed in the last subchapter. The work is mainly aimed at blueberry fruits (Vaccinium myrtillus) and vine grapes (Vitis vinifera). Other berries are briefly mentioned as well, such as cranberries, raspberries, blackberries and currant. The goal of this work is to create a diet that would provide a functional daily amount of polyphenols. This amount can be compared to 120 ml of native blueberries. Only products, consumable without cooking, are used in this diet. The second goal is to chart, which food products are adequate source of polyphenols. On the basis of these goals, research inquiries were defined. Research inquiry No. 1: What amount of polyphenols is contained in berries, especially blueberries. Research inquiry No. 2: Which food products (without cooking) are ideal to be included in diet, to provide adequate daily amount of polyphenols. To asses polyphenol content in blueberries, analysis of polyphenol content acquired through GEOMED program from Nemocnice České Budějovice a.s., department of clinical pharmacology, was used. Assessments were made using the HPLC method (high performance liquid chromatography) in cooperation with the biological centre AV ČR in České Budějovice. Thanks to this analysis, the first inquiry can be answered. An interesting phenomenon is discovered, that frozen blueberries have higher polyphenol content then cooled ones. Malvidin (malvidin-3-6"-acetoyl glycoside) was chosen as priority anthocyan since it is most represented in the blueberry. Remaining information about polyphenol content in certain food products were drawn on Joseph Maroon´s book, MD, FACS (2010). The practical part involves a diet for 14 days, which meets the given requirements. The results clearly show that it is possible to create a diet rich in polyphenols. A list of blueberry products accessible to everyone is included. Blueberry dairy products, jams, beverages, fruit salads and fruit pyre have been analysed. The list shows which product contains the most polyphenols. The required amount can be achieved by the proper combination of given products. The ideal one proved to be 200 ml of red wine and one blueberry dairy product (with the malvidin value higher than 64). Findings in this paper will serve to promote polyphenol awareness and itseffects. This diet can be used in nursing homes, where their positive influence on equilibrium can be applied. It can also be used in spas and other alimentation related facilities.
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Individual human odor as a forensic trail in criminal proceeding made by contact or contactless transmission and its resistance to physical agents
Santariová, Milena ; Bartoš, Luděk (advisor) ; Chmelíková, Eva (referee)
The dissertation thesis consists of four papers that have been published in scientific journals.
Study n. 1
The need to recover evidence from water is quite common in criminal investigation. The article deals with the possibility to collect human scent from such objects and with the ability of specially trained dogs to match such scent samples with scent samples collected from detained suspects. During an experiment, designed as a blind one, it has been proved, that human scent can survive on submerged objects and can be later used for scent identification.
Study n. 2
To collect odors the Czech Republic Police use special fabric sorbents manufactured under the registered mark ARATEX. Before use the fabric sorbent is treated by water vapor sterilization. After the scent identification the sorbent is destroyed. The goal of the study was to verify if the vapor sterilization is effective enough to remove human scent that has already penetrated into the sorbent structure or in other words if the sorbent can be exposed to vapor sterilization and then used again. Specially trained dogs were used to match starting odors with target odors in the line-ups. The results showed that dogs are able to correctly match human odors even after they have being exposed to vapor sterilization.
Study n. 3
The purpose of the study was to determine the temperature at which the human scent is degraded so that a dog would not be able to identify it. In contrast to expectations, eight dogs used in the experiment almost flawlessly identified human scents from five scent donors exposed to temperatures of 100°C, 200°C, 300°C, 400°C, 500°C, 600°C, 700°C, and 800°C. Only two of the dogs were able to identify 5 of 15 scent samples exposed to 900°C. No dog identified a scent exposed to 1000°C. Our study verified heat survivability of human scent far beyond existing expectations.
Study n. 4
We tested the hypothesis that if odor fallout (the release of a human odor onto an untouched object) in human subjects exists, then holding a hand above an absorbent will produce a detectable scent which will be subsequently matched in a detection test by trained dogs. Scents were collected from seven males to sterile cotton absorbent squares. The left hand was used to get the control scent and the right hand served as the target scent. Each experimental subject was sitting and his left hand was laid down on a cotton square for 3 min. The right hand was held 5 cm above another cotton square for 3 min. The scent identification was done by two specially trained police German shepherds. Both dogs performed 14 line-ups and correctly matched the collected scents of all test subjects. The results suggest the existence of human odor fallout, whereby a human scent trace is left by humans even if they do not touch an object.
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E-learning support for lifelong training
Žažo, David ; Husa, Jiří (advisor) ; Marek, Marek (referee)
The bachelor thesis deals with the process of creating an e-learning course for the personnel operating with the camera system of the municipal police in Chomutov. This course is created on the base of need to introduce the controlling software IndigoVison Control Center to the current employees and also to the new employees. During the course, the participants get familiar with the features, options, and operating principles of the software. At the end of the course, the knowledge gained by completing the course is validated by the final test. The entire course is evaluated, based on test results and interviews with the participants. The theoretical part deals with e-learning, it mentions the advantages and disadvantages as well as the specifics associated with adult education. The practical part is a detailed presentation of the course, which is fully implemented in an environment of MoodleCloud and it is practical result of the thesis.
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DCTOOL-A3
Bakule, Lubomír ; Papík, Martin ; Rehák, Branislav
DCTOOL-A3 is a documentation of Matlab routines developed for the design of decentralized control of large scale complex systems. The current beta version covers three areas as follows:\nReport 4.1 deals with the event-triggered control design for unstructured uncertain systems. Both non-quantized and quantized feedback is analyzed. The results are given in terms of linear matrix inequalities (LMIs). Logarithmic quantizer is used. Numerical example illustrates the effectiveness of the presented results.\nReport 4.2 presents a new decentralized overlapping wireless control design with a switched communication protocol. The method is applied by simulations on the 20-story building structure including the test of robustness of the methods against sensor failures and network node dropouts of a digital network.\nReport 4.3 presents the construction of a new decentralized wireless controller and a set of heuristic algorithms for evaluation of packet dropouts, sensor faults and actuator faults. The digital network operates at the standard frequency used in well-known widely-used industrial protocols. The results are tested at the Benchmark model decomposed into two disjoint substructures. The results are published. Thus, the details are omitted here.
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Controlling System and the Competitiveness of a Small Service Company
PODLAHOVÁ, Žaneta
The bachelor thesis deals with evaluating the current system of controlling and analysing of the competitiveness of a selected small company in the South Bohemian region. The business subject of this company is to provide services in the real estate industry, whose aim is to satisfy its customers. The practical part contains more detailed description of the company, including its history with the present and depicts its management system. The methods used are Porter's five forces model, PEST and SWOT analysis and competition analysis. Porter's five forces model analyses the surrounding environment and examints competitiveness. PEST analysis is a simple and widely used tool that helps you analyse the Political, Economic, Socio-Cultural, and Technological changes in business environment. SWOT analysis deals with the external and internal environment. This theory describes the strengths and weaknesses of the chosen company, and its opportunities and threats. Competition analysis deals with the main competitors of company. The theoretical part applies these method. Finally, I made the proposals which help to increase competitiveness of company.
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The effect of pyrethroid based pesticides on fish
RICHTEROVÁ, Zuzana
Pyrethroids are ones of the most used pesticides worldwides. The widespread use and high stability of pyrethroids lead to the assumption of that their occurrence in the environment could be quite frequent. They can reach water ecosystem as pollutants. Residues of pyrethroids are not only detected in the water column, but also in sediments and in fish tissues. The first study was devoted to the product Nexide containing 60 g.l-1 of active substance gamma-cyhalothrin. Tested Nexide concentrations were 5, 25, 50, 100, and 250 µg.l-1. Early life stage test was used.Common carp (Cyprinus carpio) was tested. There were significant mortalities in all concentrations except the lowest concentration during the trial. The lowest concentration tested 5 µg.l-1 only caused a slightly increased mortality. This lowest concentration influenced the growth in length and weight negatively, decelerated ontogenetic development, and made the body surface of the individuals darker. Histopathology of individuals from this concentration revealed dystrophy in liver. Examination of kidney, intestine and gills did not show significant histopathological differences compared with control. The evaluation of selected parameters of oxidative stress demonstrated a significantly higher activity of detoxification enzyme glutathione-S.transferase (GST) and a significantly lower activity of defensive enzyme glutathione peroxidase (GPx) compared with the control group. The other examined parameters of oxidative stress such as catalase (CAT), glutation reductase (GR), and lipid peroxidation determined by using the thiobarbituric acid-reactive substances (TBARs) were comparable to the control group. Changes in oxidative stress parameters suggest that exposure of the organism to the product Nexide in the given concentration leads to dysbalance of defensive enzymes. The second study was devoted to the product Cyperkill 25 EC containing 250 g.l-1. Tested Cyperkill 25 EC concentrations were 7.2, 36, 72, 144, and 360 µg.l-1. The procedure of the trial was the same as the preceded one. There were 100% mortalities in all concentrations except the lowest concentration during the trial. The lowest tested concentration 7.2 µg.l-1 allowed 90% of individuals to stay alive till the end of experiment. The lowest concentration influenced the growth in length and weight negatively and decelerated ontogenetic development compared with the control. Any individual exposed to this concentration did not reach juvenile stage until the end of the trial. Dark pigmentation was visible in 68% of these exposed individuals on the last day. Similar darkening was visible in individuals from higher concentrations shortly before death too. Histological examination did not revealed significant changes in intestine, liver, kidney, and gills compared with the control group. Evaluation of selected parameters of oxidative stress demonstrated significantly lower activities of GST, GR, and GPx. Activities of CAT and TBARS were comparable with the control group. Changes in oxidative stress parameters suggest that exposure of the organism to the product Cyperkill 25 EC in the given concentration could induce oxidative stress and interfere with the activities of antioxidant enzymes. The presented thesis summarises actual data about pyrethroids and their influence on fish. The demonstrated effects confirm high susceptibility of early developmental stages of fish to tested pesticides. When interpreting the results, we have to take into account the fact that studies showed this risk even on single pyrethroid substances. But water organisms are exposed to many other more or less toxic products and substances in a real environment. These xenobiotics could react with each other and their mixture could even potentiate negative effects. The performed studies also clearly show the significant differences in the sensitivity of embryonic and embryolarval tests.
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Ability of dogs to discriminate human odor exposed to chemical detergent
Čajágiová, Martina ; Vyplelová, Petra (advisor) ; Jaroslav, Jaroslav (referee)
In the recherche part of our thesis we familiarize ourselves with the human odor, theories of its origin, definition, anatomy of human skin and odors, composition of individual human odors and with odor secretion. We also look closer on the topics of odor absorbent and its use in the world, the transmission of odors to odor sensors and securing of scents. This section of our thesis discusses the resistance and survival abilities of odor, scent identification method and its history, organic acids, and defines the application of laundry detergents and ultrasonic washers. Aim of our thesis was to verify the relevance of the use of chemical detergents in the purification process when working with scents. We tried to verify the ability of detergents to degrade the human scent on odor absorbents to such an extent, that specially trained dogs would not be able to identify it. Our experiment was following a precise determination methodology. In the first phase samples were collected from the hands of targeted persons to a scent carrier - a metal cylinder (extirpate odour), by one researcher. In the second phase samples were collected from the body to a textile carrier, by another researcher. The metal scent carriers were processed. Some of them were left as they were (control sample) and some were exposed to chemical detergents with and without usage of an ultrasonic washer.
In the experiment where we tried to identify the odour samples, six bitches of German shepherd were used. They were specially trained for odour identification. Each dog was let three times to identify the target scent exposed to a detergent and three times to identify the target scent not exposed to a detergent. Target smell was randomly deposited between other samples and its position was changed, so that the handler did not know its position and thus was unable to affect the work of his dog. The indicator of positive identification was a sign the dogs were taught - to sit or lay in front of a sample.
Any dog was unable to identify the scent which was exposed to chemical detergent and all dogs identify the scent unexposed to a chemical detergent. Our experiment has shown that the use of chemical detergents in the purification process when working with scents is relevant to the degradation of individual human scent.
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