|
| |
|
|
Vliv biologického a chemického ošetření na výskyt biogenních aminů u vojtěškových siláží
Remerová, Pavla
In the experiment, the influence of two different biological silage additives (Lactococcus lactis (NCIMB 30117), Lactobacillus plantarum (DSM 16568), Enterococcus faecium (DSM 22 502 / NCIMB 11181), the enzyme xylanase EC 3.2.1.8.) And chemical (43% formic acid 30% ammonium formate and 10% propionic acid and 2% benzoic acid) at a concentration of biogenic amines, nutrient content, evaluation of the quality of the fermentation process and microbiological parameters for alfalfa silage (Medicago sativa). Silage fermentation time was 90 days. Biological silage additive significantly increased incidence of putrescine (51%; P <0.05), lactic acid (11%; P <0.05), protein (11%; P <0.05). Conversely, there was a significant reduction cadaverine (29%; P <0.05), histamine (57%; P <0.05), spermidine (15%; P <0.05), spermine (55%; P <0.05), acid acid (40%; P <0.05), ethanol (55%; P <0.05) ammonia (25%; P <0.05), and ash (9%; P <0.05). In the group treated by chemical silage additive is statistically significantly increased histamine content (63%; P <0.05) and tyramine (34%; P <0.05). Decreases in the concentration of putrescine (18%; P <0.05), cadaverine (55%; P <0.05), spermidine (47%, P <0.05), spermine (45%; P <0.05), lactic acid ( 16%; P <0.05), acetic acid (46%; P <0.05) ammonia (59%, P <0.05), ash (13%, P <0.05) and fat (24%, P < 0.05). From a microbiological point of view have been affected by the lactic acid bacteria, molds, yeasts, enterobacteria and total number of microorganisms. The results show that to maintain hygiene and healthy silage is preferable to use biological and chemical silage additives. For alfalfa silage inoculant chemical has higher efficiency in maintaining health security silage compared with biological inoculant
|
|
|
Plevele ozimé pšenice a technologie pěstování
Liška, Jakub
The aim of this thesis was to evaluate the occurrence of weeds in the winter wheat field at the field experimental station in Žabcice, depending on the technology of cultivation and on the preceding crop being cultivated. Weed infestation was evaluated using a numerical method on an area of 1m2. As a variant of soil preparation, conventional, minimizing and direct sowing technologies were used. Preceding crops for winter wheat were alfalfa and maize for silage. The evaluation took place in the years 2017-2018. The statistical processing of the evaluation of the number of individuals of all species in the winter wheat field was performed using the Statistica.cz computer software. In order to determine the effect of the observed factors on the weed species which occurred in the field experiments, multivariate analysis of ecological data was performed by using the Detrended Correspondence Analysis (DCA). In addition, Redundancy Analysis (RDA) and Canonical Correspondence Analysis (CCA) were used. The results show that cultivation technology and preceding crops affect weed species spectrum.
|
|
|
Hodnocení genetických zdrojů českých původů vojtěšky seté (Medicago sativa L.)
Knotová, Daniela
In 2009 the field experiments with the Czech varieties of alfalfa (Medicago sativa L.) were established on the two experiment areas. The evaluation took place in 2010 and 2011 in the small plots and in the individual outplanting. The size one plot was 10 m2. The main objective was to determine the similarities in the Czech collection. The morphological, yields, and biological traits were evaluated. The same varieties were evaluated using SSR markers. The Clusters analyses with the morphological and yields traits divided the set of alfalfas to the two groups and individual group with only one variety Palava. The Cluster analyse created with SSR markers divided the set also to the two groups and individual group with Jitka variety. The result of the both analyses was the similarity between Morava and Niva. In addition the intra-varietal variability was evaluated. A low intra-varietal variability was found out in cultivars Jarka, Holyna and Morava. On the other hand, however, the cultivar 'Litava' showed a high degree of imbalance. Using the correlation coefficients were found the close relationships between the characters, a direct correlation between the traits "the length of the stem" and "the number of the pods in the inflorescence". The absolute independence was found between "the length of the inflorescence" and "the number of pods per 100 flowers".
|
|
| |
|
| |
|
| |
|
| |
|
| |
|
|
Identification and sequencing genom of a new virus infecting lucerne
BEČKOVÁ, Martina
Samples of lucerne plants characteristic with local necrosic lesions, leave malformation and yellow spots on leaves were investigated with transmission electron microscopy. Virus particles observed there were filamentous ones of 600 to 700 nm long. Nucleic acid was isolated, transcribed and amplified using PCR. Genus-specific primers were designed based on reverse genetics from the highly conserved genes for carlaviruses, potexviruses and potyviruses. Successful amplification with carlavirus-specific primers, sequencing and comparison with sequences in GenBank database revealed presence of a carlavirus. This was later identified by nucleotide sequence comparison as a new isolate V4 of Alfalfa latent virus. Specific primers for isolate V4 were designed in a coat protein position. Half of the genom of this virus was obtained with PCR and PCR modified amplifications and compared with sequences of Alfalfa latent virus and Pea streak virus from GenBank.
|
guest ::
