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Pollution produced carp general, depending on the fullness of the digestive tract.
HAVLÍNOVÁ, Šárka
The aim of my bachelor thesis was to determine and compare the differences in the levels of pollution produced by fish through their metabolic activities depending on the fullness of their digestive tract. The pollution production was detected in 4 groups of fry of the common carp: 1) the fish had no access to food for 2 days before the beginning of the experiment; 2) the fish did not eat for 10 days before the beginning of the experiment; 3) the fish had a limited food intake (pellets KP1) before the beginning of the experiment; 4) the fish accepted spontaneously feed (2% by weight of the stock). The experiments were carried out in laboratory conditions. In the beginning of the experiment, the fish of individual groups and known weight were placed in experimental aquariums filled with 20 l of tap water. The experiments took 24 hours and after that the fish were moved back into its origin tank. Water from the experimental aquariums was filtered through a screen with 40 micrometr mesh size. Both, filtered water ("aqueous phase excrements") and solids ("solid phase excrements") were analysed. Solids were analysed for a content of dry matter, total phosphorus (P) and nitrogen (N). Filtered water was analysed for a concentration of suspended solids, total nitrogen, total ammonia, total phosphorus, chemical oxygen demand (CODMn, and CODCr) and biochemical oxygen demand (BOD5). Aqueous phase excrements was filtered again through a glass filter (1.2 micrometr) and the filtrate was analysed for concentration of ammonia nitrogen [N (NH3 + NH4+)], nitrate nitrogen [N-NO3-] and orthophosphate phosphorus [P-PO43-]. Pollution detected in solids and in filtered water was related to 1 kg of live weight of fish per day. The parameters characterizing pollution excreted by fish achieved in the 4th group i.e. fish, which accepted spontaneously feed the highest values. The fish of this group secreted on average of 4,154 mg.kg-1.day-1 of suspended solids (particles 1.2 micrometr), 105,5 mg.kg-1.day-1 of total phosphorus and 1,342 mg.kg-1.day-1 of total nitrogen. In the aqueous phase excrements, there was detected on average of 637 mg.kg-1.day-1 of ammonia nitrogen 4.9 mg.kg-1.day-1 of phosphate phosphorus.4,561 mg.kg-1.day-1 of CODCr, 2,634 mg.kg-1.day- 1 of BOD5. The values detected in the other groups were times lower.
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Analysis, treatment and prevention of colic disease of horses
Míšková, Tereza ; Navrátil, Jan (advisor) ; Zita, Lukáš (referee)
This bachelor thesis is about equine colic diseases, which are frequent subject of discussion. Considerable part of the thesis is dedicated to digestive tract of equine and to nutrition. Considered the range of the thesis, there are described chosen diseases of stomach, small intestine, appendix, small and large columns, rectum etc. with colic symptoms. Thesis is analysing a prevalence of equine and causes that are leading to illness, such as abdominal pain. Another part is about first aid that are owners and medical assistants able to provide to their horses and then clinical examination made by veterinary doctor. These pages are dealing with conservative and chirurgical treatment and subsequent recovery. Necessary part is prevention of colic diseases. Due to zootechnics field of study is thesis taken from the breeding point of view.
This thesis Analysis, treatment and prevention of colic disease of horses is overview of todays accessible literature. Completed and actualized todays literature about colic issues.
Next target, after reading the literature, is to find a conclusion, why occurrence of colic diseases is such a frequent problem. What reasons are triggering the colic and if we can lower the number of colic cases.
Selected issue is a large topic, for the nature of the thesis it cannot be described in the full range, therefore the author described the main information in a brief text, leading directly to the subject, which were necessary for understanding the issues. From the studied sources was find out, that even that there are many anatomical peculiarities, source of colic diseases is unfortunately mostly caused by inadequate care of horses from their owners and caregivers. Minimalizing these risks depends on the level of particular breed and is completely necessary to take care of its prevention.
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The occurrence of gastrointestinal nematodes to anthelmintic drugs in goat herds
Císlerová, Markéta ; Vadlejch, Jaroslav (advisor) ; Kyriánová, Iveta Angela (referee)
Parasitic nematodes are a cosmopolitan distribution group helminths causing the infections, which cause deterioration of health of infected animals. Frequent are indigestion e.g. diarrhea, dehydration, loss of appetite, but also to anemia. Gastrointestinal nematodes also have an impact on economic losses for farmers due to reduced production of milk and meat, the more difficult pregnancy rates, abortions, deaths during severe infections and high treatment costs. These consequences parasites worsen the overall welfare of animals, which should not be completely overlooked.
To eliminate gastrointestinal infections with broad use modern anthemintic of the following groups: benzimidazoles, imidazoles and macrocyclic lactones aminoacetonitrilové derivatives (AADs). AADs is a relatively new group of broad-spectrum anthlemintic. As an adjunct in the treatment available today increasingly researched, plants and their extracts anthelmintic effect.
After a few years since the introduction broad spectrum anthelmintic occurred first mention of resistance. Resistance is defined as the ability of the parasite to survive treatment with the recommended dose of anthelmintics that would normally be lethal to the parasite. Rapid development of resistance has caused particularly wrong drug administration procedures for the failure of the administration and also the fact that resistance is based genetic property. It is therefore very important along with the development of new effective drugs also strictly follow the prescribed procedures in the treatment.
Goats were previously treated with the same doses of anthelmintics like sheep and chronic administration of subtherapeutic doses of anthelmintic lead to faster development of resistance to anthelmintics. This was problematic, as it is known, goats have a faster metabolism and it contributed to the expansion resistance in goats.
The early detection resistance is needed to improve detection methods. Emphasis should be placed on simplicity, availability and cost efficiency that could be used directly to the farmer.
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Characterization of bifidobacteria and lactobacilli isolated from intestinal tract of wild pigs
Šimurka, Julius ; Rada, Vojtěch (advisor) ; Švejstil, Roman (referee)
The microbiota of the gastrointestinal tract is considered as "acuqrated organ" located in a host organism. There are many performs of specific functions. The microbiota composition changes during the life of the individual, depending on his environment and nutrition. The process of identification and characterization of the bacteria from the intestinal microbiota is an important prerequisite for understanding the function of this "acuqrated organ".
This work deals with the anatomy and physiology of the digestive tract (DT) of pigs and evolutionary and eating differences of wild and domesticated pig, for understanding the natural environment of bacteria from DT, which in turn affects the bacterial diversity of the intestinal microbiota. Also mentioned is the process of domestication, which influenced the present form of domestic pig. The work includes the characteristics of lactobacilli and bifidobacteria, which contains the descriptions, taxonomy, metabolism and habitats. The work also shows the insulating media used in practice for the isolation of bifidobacteria and lactobacilli from DT of pigs and methods of their identification and related methods of characterization.
The available literature confirms the differences in the composition of microbiota DT between wild and domesticated pig, that correlate with other studies which study microbiota diversity of other domesticated and wild animals. The diversity studies of humans microbiota, also confirms the broad spectrum of bacterial species in primitive tribes of humans compared with urbanized man.
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Helminths of the European otter
Seemannová, Kateřina ; Vadlejch, Jaroslav (advisor) ; Lukáš, Lukáš (referee)
The target of this study was to explore a distribution and prevalence of parasitic helmints in population of wild European otters in the Czech Republic. For this purpose organization ALKA wildlife o.p.s. provided 15 cadavers of these animals. This gained experimental material was consequently subjected to a detailed parasitical examination. All examinations and analises were made in the parazitical laboratory of Department of Zoology and Fishers, Faculty of Agrobiology, food and natural resources in Czech University of Life Sciences in Prague. The discovered parazitic organisms were subsequently isolated and determined. This autopsy showed the occurrence of parasitic flukes of genus Metorchis. Prevalence was counted 13.33 % (two positive samples from all fifteen samples). In the first sample, there were found 17 flukes, in the second sample, there were found 11 flukes. The place of discovery of this samples was southern Bohemia. The occurence of other expected representers of tapeworms, roundworms and acanthocephala were not confirmed. Results can be disorted because of low number of examined biological material. But getting bigger amount of material is a problem because the European otters are protected by law.
The results show that in population of wild European otters occur parasitic flukes of genus Metorchis. Since this is the first mention of their occurrence in the Czech Republic, it would be appropriate to study this issue from now to get more results that would be more exact.
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Effect of prebiotics on survival of bifidobacteria in digestive tract
Štěrba, Vojtěch ; Vlková, Eva (advisor) ; Jitka, Jitka (referee)
The gastrointestinal microbiota of livestock is a complex and diverse ecosystem, which strongly depends and correlates with animals health. One of the many ways, how to improve the composition of this microbiota in the gastrointestinal tract, is application of probiotics, prebiotics or an appropriate combination called as synbiotics. Using subtle interventions can be achieved health benefits, increase
of some indicators livestock performance and improving the economic situation
of farming.
The aim of the thesis was to find out the effect of prebiotics on the currently applied probiotics and the composition of the intestinal microbiota. Three groups
of calves were studied. In the first one the mixture of five strains of rifampicin resistant variants of Bifidobacterium (RRBif): Bifidobacterium animalis ssp. animalis (2 strains), B. choerinum, B. thermophilum and B. longum ssp. suis was fed to 2 days old calves in a form of fermented milk. In the second group probiotics were adminestered together with prebiotics mixture contains fructooligosaccharides and galactooligosaccharides everyday. The third group without synbiotic treatment was used as a control. The numerous of main bacterial groups in gastrointestinal tract were determined by cultivation.
Our results showed that applied bacteria were able to survive the passage through the gastrointestinal tract of calves. Administered bacteria persist in counts more than 107 CFU/g in the group of calves, that were fed only by probiotics and in numbers 108 CFU/g in the group of calves, that were treated by synbiotics. Furthermore a slow decrease of bifidobacterial numbers to 104 CFU / g was observed in both groups. Significant difference (p = 0.05) between the count of RRBif in probiotic and synbiotic group were determined only two days after administration of that additive. Administration of probiotics and synbiotics to calves increased the count of naturally occurring bifidobacteria, but these differences were not significant.
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