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Characterization of Natural Biostimulants and Their Effect on Soil Properties
Hanzelková, Kateřina ; Bělonožníková, Kateřina (advisor) ; Smrček, Stanislav (referee)
Biostimulants represent an environmentally friendly way of protecting plants and supporting their growth. Here, fermented nettle was characterized in terms of antioxidant properties, protein content, and activities of selected enzymes. Furthermore, this study focused on the effects of biostimulants (fermented nettle and vermicompost) on soil properties. Fermented nettle showed high content of phenolic compounds and high antioxidant capacity. Activity of proteases, as well as acid and alkaline phosphatases was successfully determined in fermented nettle. Alkaline phosphatase activity was significantly higher than that of acid phosphatase. Protease, endo-β-1,3-glucanase, and total peroxidase activities were determined in soils of plants treated with biostimulants - fermented nettle (N), vermicompost (V) - and compared with control (C). All groups of plants were also exposed to drought stress (D). Application of biostimulants did not affect protease activity in watered groups but D groups showed a significant increase in proteolysis. Endo-β-1,3-glucanase activity was higher in the groups treated with biostimulants and drought did not affect the activity. The group VD showed significantly higher total peroxidase activity than other groups. The protein content in groups treated with biostimulants was...
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Drought stress response of European beech seedlings monitored using physiological and optical indicators.
Červenka, Ondřej ; Lhotáková, Zuzana (advisor) ; Gebauer, Roman (referee)
One of the manifestations of global climate change is an increase in extreme temperatures and drought waves, while forecasts of future development indicate deepening negative impacts. The effect of these abiotic stress factors is reflected in the physiological state of the vegetation. It is therefore necessary to find suitable stress indicators that will help detect the worsening of physiological state of vegetation in time series. Indicators can be detected not only by physiological methods, but also optical ones, for further use of vegetation monitoring by remote sensing methods. Forest beech (Fagus sylvatica L.) is a native tree in our context, currently widely used in forestry for planting more species-varied communities of trees that are more resistant to the effects of climate change. The aim of the thesis was to study the responses of beech to drought stress using physiological and optical indicators. Beech seedlings (planting material from the nursery) were placed in containers in the experimental greenhouse for two experimental seasons, water supply was mediated by drip irrigation. The experimental material was divided into three groups with graded irrigation (control, moderate drought and drought). The watering regime consisted of three periods of drought and recovery phases with uniform,...
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Impact of nitrogen fertilization and drought on gliadins content in winter wheat
Odstrčilová, Eva ; Obruča, Stanislav (referee) ; Hrstka, Miroslav (advisor)
In this diploma thesis the impacts of both nitrogen fertilization and drought on the content of proteins of the gliadin fraction in case of a winter wheat variety Tiguan were observed. Selected samples were cultivated at two locations in a total duration of two years. Two different conditions were selected: one without the nitrogen fertilization (0 kg N/ ha) and the other with the nitrogen fertilization in 140 kg N/ ha concentration. Second observed factor was the drought which was ensured by roofs above the crops and their cultivation in common climatic environment. Gliadin fraction was separated by the A-PAGE method and the protein quantification carried out by a computer densitometry. Such nitrogen fertilization caused a significant increase of gliadins, especially -1,2 and -5 fractions. Stress induced by the drought caused an increase of gliadin content compared to the control sample, particularly in case of - and - fractions. The most important factor influencing the gliadin content in grains was therefore the nitrogen fertilization in a dry environment. The observed increase of gliadin content was lower in case of the sample which was fertilized in a humid environment than in case of the sample which was fertilized in the dry one.
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Odrůdová variabilita vitality osiva máku a její souvislost s velikostí kořenového systému
Jedličková, Žaneta
The thesis deals with seed vigour testing of 10 selected poppy genotypes. The aim was to determine the tolerance of poppy seed to selected suboptimal abiotic factors such as drought and temperature, and to clarify its relationship with the ability to form the root system or the final product - poppy seed in the yield-forming process. The laboratory part of the experiment was carried out at the Department of Crop Science, Breeding and Plant Medicine at Mendel University in Brno. Observations and measurements were carried out in a field experiment in the municipality of Chvalíkovice in 2022 under the direction of OSEVA PRO s.r.o. The results confirmed the expectation that the number of germinated individuals decreased with increasing drought intensity. Significant differences between genotypes were found when testing for vigour. Differences in root system size and seed yield were also found. An interesting finding of this work was the data showing a higher number of germinated individuals at 10 °C compared to testing at 20 °C. A statistically significant relationship was found when poppy seed vigour data were correlated with poppy root system size. The more vigorous the seed, the more able the plant is to form a robust root system in the early stages of growth. However, this relationship is no longer clearly reflected in the yield of poppy. There is no conclusive relationship between the vigour of poppy seed and its subsequent seed yield. A myriad of external factors enter into the poppy seed yield process, so it cannot be said that a highly vigorous seed will produce a large quantity of quality poppy seed.
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Vitalita a růst smrku ztepilého ve vertikálním gradientu České republiky
Kovačević, Vuk
Long periods of drought and irregular precipitation are common phenomena in the 21st century, which, along with the management of Norway spruce (Picea abies (L.) Karst.) outside its natural range in Europe, have led to the physiological decline and mortality of entire spruce stands. Drought significantly impacts tree growth and vitality, and these adverse effects are better monitored through tree water deficit (TWD) or water content in the tree. This study focuses on monitoring and analyzing the correlation between radial stem growth and climate changes at altitudes ranging from 380 to 972 meters in the Czech Republic. Growth was monitored using automatic dendrometers for five years (2016–2020). The negative impact of climate changes on radial growth is more significant at lower elevations below 650 meters above sea level, where the highest TWD occurred. This means that managing Norway spruce at lower elevations is risky and requires a change in management practices.
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Vliv smíšení porostu na dynamiku růstu borovice lesní (Pinus sylvestris L.) ve Finsku
Vyčítalová, Hana
Mixed stands excel in their species richness and they theoretically have a better precondition for coping with disturbances than monocultures. On the other hand, there is interspecies competition, which may result in the oppression of individual species in the ecosystem. A total of 12 research plots in the province of North Karelia (Finland) were selected to approximate the effect of forest mixing on the growth of Scots pine (Pinus sylvestris L.). In growing season 2018, stem volume changes and drought stress responses were observed in monocultures and with the admixture of one or both of the species Norway spruce (Picea abies L.) and Silver birch (Betula pendula Roth.). The measurement of volume changes was performed using point dendrometers installed on individual pines, the temperature and soil moisture were monitored by a TMS-4 sensor located in the soil in the middle of each research area. It was found that in all cases, Scots pine grew only in dependence on rainwater, not groundwater. It was discovered that individuals in monoculture grew up to twice as much as in both types of mixed stands. They also showed a better ability to saturate the tissues with water after it had been spent for transpiration during the July drought. In August, however, the response to drought stress was comparable in monocultures and mixed stands. The measured results show that interspecific competition may have a negative effect on the growth and drought tolerance of Scots pine in the short term. However, at a time of escalating extreme climate events around the world, this issue needs to be further explored in order to create optimal conditions for the growth of future forests.
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Assessment of drought response in local Scots pine ecotypes using anatomical, biochemical, and fluorescence stress markers.
Štěpánová, Kristýna ; Lhotáková, Zuzana (advisor) ; Stejskal, Jan (referee)
Global climate change and associated global warming is already a problem for temperate forest ecosystems and will continue to exacerbate the problem in the coming decades. Many species will have to adapt to a multifactorial combination of stresses, with increases in average air temperature, frequency and intensity of precipitation increasing drought episodes and drought-affected areas will enlarge. It is necessary to find ways to help the landscape cope with these intensifying phenomena. The possibility of planting suitable tree species that can manage water efficiently and survive even in periods of intense water scarcity is an option. The drought tolerance of trees is related to their phenotypic plasticity and ecotypic differentiation. Scots pine (Pinus sylvestris L.) is characterised by modest ecological demands on the environment and climate of the habitat, is phenotypically plastic and relatively drought resistant. The objectives of this study were 1) to evaluate and compare the response of different Scots pine ecotypes to drought stress due to reduced water availability using anatomical, biochemical, and fluorescence indicators of physiological status, and 2) to determine the biochemical phenotype of selected genotypes within a local Scots pine ecotype in a seed orchard in terms of...
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Effect of year on field germinability of opium poppy seeds
JELÍNKOVÁ, Olga
The aim of this thesis was to perform an experiment with opium poppy control and drought stressed plants. Gained seeds were tested for germinability in normal conditions and during simulated drought stress induced with PEG 6000. It was found plants for seed production provides less seeds with lower quality, but the vigour and germinability of these seeds are better. In this thesis I also tested the method of surface plasmon resonance as potentialy suitable method for dehydrin accumulation analysis. I managed to verify method for termostable proteins isolation and at the same time I confirmed, the SPR method is potentially usefull method for dehydrin accumulation analysis in opium poppy seedling plants.
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Study of common bean drought response with the use of leaf optical properties
Svitáková, Lenka ; Lhotáková, Zuzana (advisor) ; Zámečník, Jiří (referee)
The negative effects of climate change have affected the conditions of agriculture areas, which leads to hampered cultivation of crops, including the common bean's cultivation. Changes, such as extreme temperature swings and lowered water availability in soil create a big challenge for today's agriculture in seeking solutions for safeguarding the food security for all people on our planet. The aim of this thesis was to study a wider range of genotypes of common bean to quantify their resistance against lowered water availability in soil, and to establish new approaches for detecting drought stress with the use of leaf optical properties. From the methodological point of view, this thesis connects the leaf optical properties with anatomical and biophysical leaf traits. The common bean (Phaseolus vulgaris) and the tepary bean (Phaseolus acutifolius) were employed as the model organisms. Plants were cultivated in two differing environments - in a greenhouse setting at the Faculty of Science of the Charles University in Prague and on the experimental fields in Colombia at the international research institute for tropical agriculture - Alliance of Biodiversity International and CIAT. There were 48 genotypes included into the field experiments in Colombia. These included genotypes from both Mesoamerican...
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Vliv vysychání sadebního materiálu buku lesního a smrku ztepilého na jeho ujímavost a růst po výsadbě
Rašková, Pavlína
This bachelor thesis focuses on bad handling of bare-rooted and containerized seedlings of European beech (Fagus sylvatica L.) and Norway spruce (Picea abies L.). The aim of this thesis was to find out what influence the different length of drying of the root system of used planting material before planting, its follow-up and growth after planting and to determine critical values and times when plants are able to accept drought stress without significant losses and degradation of growth. The plants were planted as a freshly plants and after 1 and 2 weeks of establishment. The stress by drought was taking place in the shade, near the planting site for 0 to 48 hours. Along with planting, plants were tested to determine the water content of the root system or rootballs. The tested methods were the weight loss of the fine roots under constant heat stress, the measurement of the weight loss of the rootballs and the measurement moisture of the rootballs, using WHT 860, SM 300 and EMS sensors. The EMS sensor was found to be unpromising due to the inability to measure values in plants with too-dried rootballs whose plants did not show significant losses or degradation at the end of the growing season. Furthermore, it has been found that the seedlings of European beech are more predisposed to stress by drought, especially if the plants have before planting badly morphological conditions. On the contrary, the seedlings of Norway spruce are resistant to long-term stress.
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