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Genes of early meiotic prophase I of spermatogenesis in house mouse
Škaloudová, Eliška ; Trachtulec, Zdeněk (advisor) ; Forman, Martin (referee)
Meiosis is an essential cellular process that is necessary for gamete formation in all sexually reproducing organisms. This work is focused on the description of the genes of early stages of meiotic division in males of a mammalian model, the house mouse. The first part summarizes meiosis focusing on prophase I, which is longer than prophase II. Prophase I is divided into five stages, namely leptotene, zygotene, pachytene, diplotene, and diakinesis. Mouse spermatogenesis and its differences from oogenesis are also briefly described. The second part provides a list of genes encoding proteins required for initiation of meiotic division, pairing and synapses of chromosomes, and initiation of the catalysis of double-strand breaks. Double-strand breaks are repaired by homologous recombination, which may result in so-called crossing-over, the major source of genetic variability. The work deals with the early stage of homologous recombination and components required for this process. Localization of meiotic double-strand breaks in the genome is not random and is under the control of the Prdm9 gene, which seems to take multiple roles, such as the formation of new subspecies of the house mouse. Knowledge of the genes controlling the early stages of meiotic division is a prerequisite to understanding some of...
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Quality control of blood products
NOVÁČKOVÁ, Barbora
The main theme of this bachelor thesis is a quality control of blood products which are prepared in the Transfusion Centre in the České Budějovice Hospital. In my thesis, I focus on the rudimentary blood products manufactured from whole blood. These include resuspended erythrocytes without a buffy coat (EBR), plasma (P), mixed thrombocytes without leukocytes from a buffy coat in a spare solution (TBSDR) and mixed thrombocytes without leukocytes (TBSD). The aim of this work is a statistical analysis of quality of the aforementioned blood products used in the past 5 years. Moreover, I have devised my own quality control of the newest blood products TBSDR. The theoretical part addresses blood donors´ control, methods of blood collection, blood processing and procedures which may significantly influence the quality of blood products. I also describe the examined blood products and the quality control process. In the practical part, I deal with the procedure of examining the particular parameters of blood products´ quality control and their assessment. After the preparation of blood products, sampling is done as well as the control of both efficient and undesirable components (erythrocytes, leukocytes, thrombocytes, hemoglobin, hematocrit). Assessment of stability and sterility of blood products (hemolysis, pH, coagulation factors) is done at the end of their expiration. I will focus more carefully on the TBSDR blood product quality control which starts one month after the blood product is implemented into a production process and which lasts six months. Statistical control of the process is applied during the quality control. In the past 5 years, the quality of blood products has been satisfactory. The newly implemented blood product TBSDR is more high-quality than the previous TBDS. TBSDR provides not only patients but also transfusion centers with many advantages.
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Comparison of the occurrence of inversion at the 9th chromosome in infertile and sterile patients with literature dates
Šlégrová, Sandra ; Krkavcová, Miroslava (advisor) ; Šípek, Antonín (referee)
The pericentric inversion of the chromosome 9 [inv (9)] is the most common small-scale inversions in the human chromosomes. Some publications consider it as a variation of the human karyotype, which has no clinical manifestations. However there are mentioned the studies in this thesis that reports the inversion on the chromosome 9 in connection with the various pathological conditions, such as sterility, infertility, or some congenital anomalies. However, most attention is paid to the patients who were evaluated for the indication due to the failure of reproduction (sterility or infertility). The research was conducted in the accredited genetic laboratory GENvia s.r.o., where the data of the period 2004-2011 are collected. During this period a total of 5195 patients of variety indication reasons were examined. Totally 84 cases of them were found with inversion of the chromosome 9 with an incidence of 1.62 % laboratory. The indication diagnoses are divided into six created groups because of their quantity. One of them is called sterility/infertility. There were found 23 cases with inversion in the 9th chromosome in this group. It makes 27.38 % totally of the amount found in the chromosome inversions 9th. The occurrence of this inversion was also noticed in other indication groups: the congenital...
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Methods of family planning at Romany woman
ŠULCOVÁ, Iva
In this thesis, I addressed the issues of the Romany population, in particular family planning of Romany women. My thesis is divided into two parts. The first part is theoretical and the other is practical. The first part dealt with the history of the Romany population, Romany customs, rituals, housing, family, children, etc. These phenomena were separately applied to the multicultural nursing model - a Giger-Davidhizar model. Due to globalization and population migration, I emphasized the need to use and apply the model of multicultural nursing as an integral part of nursing care. In the second part of my thesis, I carried out my research through semi-controlled interviews with five randomly selected Romany women aged between 20-40 years. Five objectives and related research questions were defined. All the objectives were met. Research question 1, whether Romany women are aware of family planning methods, was not answered definitely. Research question 2 shows that Romany women use family planning methods. Research question 3 resulted in the finding that Romany women use hormonal contraceptives in family planning. Research question 4 explained that Romany women do not find family planning important. The respondents replied to research question 5 ? what Romany women?s needs in primary care are ? in the sense that they do not have any specific requirements. My bachelor thesis can be used as a resource for midwives who want to learn about the problems of the Romany population and family planning, as well as for working midwives who would apply the knowledge in the field of multicultural nursing his their practice, recommend adequate contraception in primary prevention and educate Romany women on when, how and under what circumstances to start a family. The general public, especially young girls, who begin their sexual life, will find here an overview of all available and used methods of contraception.
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PHYSIOTHERAPY AS A PART OF FERTILITY DISORDERS TREATMENT
ROHLÍČKOVÁ, Eva
Infertility is defined as inability of a couple to conceive a child within one year of having unprotected sex at least two times a week. In 1970s the World Health Organization classified the condition as a disease. The data indicate that approximately 20-25% of couples in the Czech Republic are involuntarily childless. There are many methods used to address this condition. One of them is a physiotherapeutic approach. The purpose of this thesis was to find new (modern) views of the issue or potentially also some of its causes, and to point to the potential use of modern physiotherapeutic approaches to infertility treatment. To achieve the objective I have focused in the theoretical section on various causes of fertility disorders and their diagnostics. Further, I have tried to map some methods used in infertility treatment. I have strongly emphasized the physiotherapeutic approach, particularly methods developed by Ludmila Mojžíšová. In the practical section I have used a qualitative research method {--} the case study. The research was conducted in the Rehabilitation and Regeneration Center in Borovany near České Budějovice. Women selected for the survey were diagnosed with infertility and palpation sensitivity of pelvic bottom muscles. Three women treated with a rehabilitation method for some types of functional female infertility. The objective of the research was to map changes in the condition of muscular dysbalances in their pelvis bottom areas. In the surveyed patients improvements were found in terms of posture, breathing stereotype and pelvic bottom function. During the treatment all the three women reported gradual improvement of subjective feelings. One of the women got pregnant during the treatment. The main contribution of this thesis is the mapping of the infertility phenomenon. From the viewpoint of physiotherapy this work may be used in clinical practice by physiotherapists involved in this area.
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Sampling of biological material in outpatient health care facilities viewed by a nurse.
SRBOVÁ, Kateřina
Sampling of biological material is one of the most frequent duties of nurses working in outpatient health care facilities. The nurse takes biological samples daily and has to respond to possible problems. Sampling of biological material is an important component of the patient´s examination and therefore it must be carried out correctly and properly. The quality of the procedure is affected by the nurse´s experience, skills and knowledge. It is important for a nurse working in an outpatient health care facility to master the sampling technique so that patients were satisfied and the samples did not get devaluated. The objective of my research was to give an account of the most frequent problems occuring when samples of biological material are taken in outpatient health care facilities, and to find out if nurses give patients enough information before the procedure. I set three hypotheses: the H I - nurse do not use protective aids while taking samples of biological material, the H II {--} nurse do not follow the recommended standards, the H III {--} nurse do not inform patients about preparations before taking samples of biological material. To achieve the objective and to verify the hypotheses the questionnaire method was used. Questionnaires were addressed both to patients and nurses. The research sample included 103 outpatients and 73 nurses from outpatient health care facilities in the South Bohemian and the Central Bohemian regions. The obtained data analysis showed that the H I {--} nurse do not use protective aids while taking samples of biological material {--} was not confirmed. H II {--} nurse do no follow the recommended standards {--} was not confirmed. The H III {--} nurse do not inform patients about preparations before taking samples of biological material {--} was not confirmed either. The results of my research reflect the fact that the most common problem is communication between a nurse and the patient. But there is more to be improved - patients education, the fixed placement of aids and the early transportation of samples to laboratories. I would like to make use of these conclusions and pass them over not only to nurses who took part in the research but also to the hospital managements. I intend to give a presentation on my research conclusions to nurses working in outpatient health care facilities.
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