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The role of microbiome in celiac disease
Tučková, Kateřina ; Konečná, Klára (advisor) ; Jílek, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Subject of study: Healthcare bioanalytics Student: Kateřina Tučková Supervisor: RNDr. Klára Konečná, Ph.D. Title: The role of microbiome in celiac disease Background: Descriptions of the celiac disease problematics. Focus on the origin of the disease and its possible connection and role of the intestinal microbiome. Define the term microbiome and point out its possible role in human health. Finally, address the attention to possibilities in the treatment of celiac disease. Main findings: The role of the microbiome in celiac disease and other diseases is a frequently discussed topic. Patients with the celiac disease diagnosis depend on a lifelong gluten-free diet. The microbiome is highly specific to and is affected by many external stimuli, which can often be affected. Current studies point to a linkage between the microbiome and many diseases, including celiac disease. Conslusions: More knowledge is needed for the complex insight into the problematics of the relationship between celiac disease and the microbiome. In general, the microbiome composition has a much more significant impact on human lives than previously thought. Intestinal microbiome imbalance is related to celiac disease...
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The effect of antibiotics on human gut microbiome and the influence of probiotics on its restoration
Hloucalová, Nikola ; Lichá, Irena (advisor) ; Ulrych, Aleš (referee)
Antibiotics are used for treatment of bacterial infections. They negatively affect not only the pathogens, but also other microorganisms in the gut, including the beneficial bacteria. Antibiotic treatment changes the proportion of good versus bad bacteria in the gut, causes a decrease in the number of commensal bacteria and leads to the overgrowth of opportunistic pathogens. We should consume probiotics during and after the antibiotic treatment, otherwise it results in an unhealthy stool and moreover it affects the immune system which then leads to physical and mental illnesses. This thesis summarizes the influence of probiotics on human gut during dysbiosis caused mainly by antibiotics.
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Changes in intestinal flora in toodlers after application antibiotics
ŠTABERŇÁKOVÁ, Jitka
In the theoretical part, I concentrated on the characteristics of the bacteria from the Enterobacteriaceae family. I followed up with aerobic intestinal flora, which we establish by common cultivation. I described the course of microbiological examination and the characteristics of antibiotics from the mechanisms of their function to their side effects. The aim of my bachelor's thesis was to establish, how the intestinal microbiome is affected after the administration of the antibiotic Klacid. In collaboration with a pediatrician, 76 rectal swabs were performed on 38 toddlers, to whom Klacid was prescribed to cure a respiratory disease. Two swabs were performed; the first performed before the beginning of treatment by Klacid and the second after the conclusion of the antibiotic treatment. Both of the swabs were sent to the laboratory of Stafila k.s. for a microbiological examination of aerobic strains. The samples were inoculated and during the next days also evaluated. Using biochemical examination, further determination of the individual bacteria was performed. I acquired microbiological examination of 76 samples of bacteria. I determined that in 76% only an insignificant change in the opportunistic bacteria occurred after the antibiotic treatment; in the next 24%, there was a recess in the pathogenic flora.
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Changes In Toddlers' Intestinal Microflora After Antibiotics In Connection To Nutrition
MRLÍKOVÁ, Tereza
The bachelor thesis focuses on changes in toddlers' intestinal microflora after antibiotics in connection to nutrition. The first aim is to inform public about the importance of healthy gut microbiota. The second aim is to determine to what extent antibiotics influence intestinal microflora. The third aim is to analyze a list of daily meals of selected toddlers with antibiotics and to propose recommendations to support the health of intestinal microflora. In the theoretical part of my bachelor thesis I focus on key information about intestinal microflora, its characteristics, abilities and benefits for human organism. I also mention effects of enviroment on microbiota, benefits of fiber as an important source of energy for bacteria and ongoing trend of antibiotics indications which have major impact on bacteria. According to my stated goals, in the practical part of my bachelor thesis I analyze stool samples from toddlers before and during antibiotic treatment, compare them and determine if the microbes changed. I simultaneously collected some lists of daily meals from toddlers' mothers and I analyze approprietness of the meals for toddlers and changes that should go along with the antibiotic therapy. I also focus on the presence of probiotic foods. This bachelor thesis can serve as an information background about antibiotic treatment. From laboratory examination we can see that the amount of aerobic bacteria in intestinal microflora changed in 15 toddlers. Altogether only 10 toddlers consumed some probiotic food at least once a day. The sufficient amount of fiber according to my estimation fulfilled 13 lists. There were 20 toddlers in total.
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Influence of nutritional measures on ulcerative colitis
Guznar, Jan ; Meisnerová, Eva (advisor) ; Hrabák, Pavel (referee)
Diploma thesis The influence of nutritional measures on the course of ulcerative colitis deals with the influence of dietary habits on the course of ulcerative colitis (especially probiotic foods), namely impact on relapses of the disease. The theoretical part is divided into two parts. The first part describes ulcerative colitis, from definition to treatment. The second part deals with the intestinal microbiome and the factors that affect it, with an emphasis on the influence of food. The semi-structured interview method based on the questionnaire was used to elaborate the practical part. The research group consisted of respondents with ulcerative colitis who were in remission and relapse. A questionnaire was filled with respondents, which consisted of questions about the course of the disease and a frequency questionnaire targeting different groups of foods. The established eating habits were compared to each other among the respondents, given the frequency of relapse of their illnesses. Research has shown that only fermented dairy products are related to relapse rate and retention of remission. For foods that contain fiber that also modulates the intestinal microbe, the relationship between food consumption and relapse has not been proven. Also, the relationship between consumption of fatty...
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The role of the gut microbiome in immune-mediated CNS disorders
Zedníková, Barbora ; Herink, Josef (advisor) ; Semecký, Vladimír (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Bc. Barbora Zedníková Supervisor: Doc. MUDr. Josef Herink, DrSc. Title of diploma thesis: The role of the gut microbiome in immune-mediated CNS disorders Human body hosts a large number of microorganisms - i.e. Archea, Eukarya, Bacteria and viruses. These microorganisms form microbiome, the total number of the microorganisms is ten times higher than the number of all human cells. Largest part of the microbiome is located in the intestine. The current development of molecular genetics revealed the close relationship between intestinal microbiome and health. Recent studies the most recent studies have pointed to a connection with the pathogenesis of various diseases. This dissertation is focused on the connection between intestinal microbiome and autoimmune diseases of the central nervous system. Research shows that the key factor are the ongoing changes in the composition of microbiome. These changes lead to increased immune stimulation and thereby to inflammatory proliferation.
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