|
The Introduction of Contact Ecotoxicity Tests for the Assessment of Terrestrial Ecosystems
Modlitbová, Pavlína ; Kráčmar, Stanislav (referee) ; Beklová, Miroslava (referee) ; Demnerová, Kateřina (referee) ; Vávrová, Milada (advisor)
This dissertation thesis is focus on using toxicity tests with testing organism - terrestrial isopod Porcellio scaber. This organism is use for toxicity assessment of selected anorganic compunds which could contaminate terrestrial ecosystem. This organism was selected for several reasons - sophisticated test methodology; well known biology of these animals and their ecological relevance. Besides classical endpoints such as mortality, change in mass of test specimens and the effect of the presence and concentration of contaminants onto food consumption; we monitored changes in the morphometric characteristics at the level of tissues and cells, the cytotoxicity and possibly bioaccumulation in various parts of the body of the organism. Selected test substance in this thesis were nanoparticles and inorganic salts. Wherein for chosen gold nanoparticles was carried out a complete study presents several levels of organization of living matter (organism, tissue, cell). For selected salts compound, this study was carried out on the behavior of organisms and classical endpoints.
|
|
Model of transport in vadose zone in vertisols under semiarid climate.
Weiss, Tomáš ; Ondovčin, Tomáš (advisor) ; Kodešová, Radka (referee)
Vertisols cover a hydrologically very significant area of semi-arid regions, and thus understanding of water flow and solute accumulation is very relevant to agricultural activity and water resources management. Previous works suggest a conceptual model of desiccation-crack-induced salinization where salinization of sediment in deep section of the vadose zone (up to 4 m) is induced by subsurface evaporation due to convective air flow in desiccation cracks. This thesis presents a conceptual model of water flow and solute transport in vertisols, and its numerical implementation. The model uses a single-porosity material but unconventionally prescribes a boundary condition representing a deep crack in soil and uses the unsaturated hydraulic conductivity as one of the fitting parameters. The numerical model is bound to one location close to a dairy farm waste pond, but the application of the suggested conceptual model could be possibly extended to all semi-arid regions with vertisols. Simulations were conducted using several modelling approaches with an ultimate goal of fitting the simulation results to the controlling variables measured in the field: water content, and chloride salinity of pore water. The development of the model was engineered in numerous steps; all computed as forward solutions by...
|
|
The Introduction of Contact Ecotoxicity Tests for the Assessment of Terrestrial Ecosystems
Modlitbová, Pavlína ; Kráčmar, Stanislav (referee) ; Beklová, Miroslava (referee) ; Demnerová, Kateřina (referee) ; Vávrová, Milada (advisor)
This dissertation thesis is focus on using toxicity tests with testing organism - terrestrial isopod Porcellio scaber. This organism is use for toxicity assessment of selected anorganic compunds which could contaminate terrestrial ecosystem. This organism was selected for several reasons - sophisticated test methodology; well known biology of these animals and their ecological relevance. Besides classical endpoints such as mortality, change in mass of test specimens and the effect of the presence and concentration of contaminants onto food consumption; we monitored changes in the morphometric characteristics at the level of tissues and cells, the cytotoxicity and possibly bioaccumulation in various parts of the body of the organism. Selected test substance in this thesis were nanoparticles and inorganic salts. Wherein for chosen gold nanoparticles was carried out a complete study presents several levels of organization of living matter (organism, tissue, cell). For selected salts compound, this study was carried out on the behavior of organisms and classical endpoints.
|