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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Chinese characters and language purification movement in Korea
 Repková, Gabriela ; Horák, Tomáš (advisor) ; Ferklová, Blanka (referee)
Chinese characters using in classical Chinese (hanmun) have long history of using in Korea. After an invention of the Korean alphabet - hangŭl begun the first attempts to using hangŭl beside hanmun. Attempts have gained strength during the centuries and in 19. century the thoughts about exclusive using of hangŭl have begun showing up. The prohibition of using characters in both parts of Korea was accepted in the middle of 20. century. The North has stopped using characters completely but teaching of characters at schools is still alive. In South Korea was later accepted that for the temporary time the characters will be using beside hangŭl. The idea that Korean language needs to be purified of various foreign elements fully show after the end of Japanese colonial administration in 1945. Various linguistics and government societies were concerned with this issue. In the North purification got stronger mainly in 60s of 20. century, in the South in 70s. Nature of language purification in the North is different from nature of language purification in the South and this led into deeper differences in vocabulary of the both states. Key words: Chinese characters, hanmun, purification, language policy, purification movement
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Plasmid DNA vaccines
 Machan, Radoslav ; Chroboková, Maria (referee) ; Rittich, Bohuslav (advisor)
Plasmid DNA vaccines are the new generation of vaccines with a great potential in prevention of many diseases. Recent studies and clinical test are aimed at prevention against cancer, hepatitis, malaria, HIV, influenza and other diseases. Recent main challenges covering plasmid DNA vaccines are associated with optimalization of each step of production and mainly purification steps allowing production of pDNA at kilogram levels. Main purification techniques used are based upon chromatographic methods, but research and development also shows other potential methods, like two-phase aqueous systems or magnetic microparticles as carriers. In experimental part of this thesis isolation of pUC 19 plasmid from Escherichia coli JM 109 (pUC 19) cell culture was performed via method of alkaline lysis. Isolation was verified by agarose gel electrophoresis. Isolated samples were purified in four repetitions with lithium chloride and magnetic microparticle carriers and the extent of purification was verified spectrophotometrically. Purified samples were visualised via agarose gel electrophoresis and results were compared.
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