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Cardioprotective action of ischemic perconditioning
Chalupová, Miloslava ; Neckář, Jan (advisor) ; Šilhavý, Jan (referee)
Remote ischemic perconditioning (RIPerC) is acknowledged to be a promising cardioprotective strategy, defined as brief repetitive periods of ischemia and reperfusion applied during ongoing myocardial infarction. This method provides protection against ischemia-reperfusion injury. Although remote perconditioning reduces infarct size, the underlying mechanisms remain unclear. The aim of this thesis is to summarize the current knowledge of RIPerC, its molecular mechanisms and protective effects on the heart.
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Effect of chronic morphine on cell survival after oxidative stress in the SH-SY5Y neuroblastoma cell line
Moutelíková, Karolína ; Hejnová, Lucie (advisor) ; Musílková, Jana (referee)
Morphine is a natural opioid which is used in medicine due to his potent analgesic and sedative effects. In the forefront of scientific interest is a chronic usage of opioids which can lead to a development of drug addiction. Morphine role in oxidative stress was described in last years. It was revealed its protective potencial by many studies. However, some studies described its pro-oxidative effect. The aim of this study was to determinate effect of chronic morphine on cell survival after oxidative stress caused by H202 analog - tBHP in the SH-SY5Y neuroblastoma cell line. The results verified morphine protective effect against oxidative stress. The highest protective effect of morphine was achieved in a concetration of 10 µM. It was desribed that morphine can induce activation of mu-opioid (MOR) and Toll-like 4 (TLR4) receptors signalling pathway on molecular level. The aim of this thesis was to evaluate the role of MOR a TLR4 in protective effect of morphine against oxidative stress by two methods. Firstly, it was used tests of oxidative stress on cell viability. The obtained results demonstrated majority role of TLR4 and minory role of MOR. Afterwards, we assesed changes in the expression of MOR a TLR4 after chronic morphine by SDS-PAGE electrophoresis. Results of these experiments did not...
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Personnel selection
Mužíková, Lenka ; Kolman, Luděk (advisor) ; Nosek, Ondřej (referee)
This bachelor thesis deals with selection of employees in the state administration. Approaching the problem of the possible presence of nepotism in selection of employees. Thesis is divided into theoretical and practical part. The theoretical part deals with the search, recruiting and selecting staff from literature. The next point of the theoretical part is the selection of employees in the local government units on response to the law n. 312/2002 Sb., which is about local government units officials and amended later laws. In the theoretical part are the social and human consequences of the choice of employees in connection with nepotism. The practical part is focused on interviews with employees of municipal office.
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