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Changes of internal organs after percutaneous exposure to sulfur mustard
Šulová, Veronika ; Červený, Lukáš (advisor) ; Vokřál, Ivan (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Veronika Šulová Supervisor: doc. PharmDr. Lukáš Červený, Ph.D. External supervisor: pplk. doc. MUDr. Jaroslav Pejchal, Ph.D. et Ph.D. Title of diploma thesis: Changes of internal organs after percutaneous exposure to sulfur mustard Sulfur mustard is a chemical warfare agent belonging to the group of blistering agents. The theoretical section of the thesis is mainly focused on the description of acute toxic effects, the mechanism of action, and deals with the current possibilities of poisoning therapy. The experimental section is focused on monitoring the effect of sulfur mustard poisoning in the liver, lung, and kidney of female C57BL/6J mice after the percutaneous administration. This work aimed to evaluate markers of oxidative stress and histopathological changes of the selected organs at 3, 5, and 7 days after the poisoning. Ferric reducing antioxidant power (FRAP) and thiobarbituric acid reactive substances (TBARS) methods were used to determine markers of oxidative stress. Histopathological changes were evaluated microscopically using the hematoxylin-eosin staining method. The airness of the lung parenchyma was also assessed by computer image analysis. First, the LD50 of sulfur mustard was...
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Detection of foreign objects in X-ray chest images using machine learning methods
Matoušková, Barbora ; Kolář, Radim (referee) ; Chmelík, Jiří (advisor)
Foreign objects in Chest X-ray (CXR) cause complications during automatic image processing. To prevent errors caused by these foreign objects, it is necessary to automatically find them and ommit them in the analysis. These are mainly buttons, jewellery, implants, wires and tubes. At the same time, finding pacemakers and other placed devices can help with automatic processing. The aim of this work was to design a method for the detection of foreign objects in CXR. For this task, Faster R-CNN method with a pre-trained ResNet50 network for feature extraction was chosen which was trained on 4 000 images and lately tested on 1 000 images from a publicly available database. After finding the optimal learning parameters, it was managed to train the network, which achieves 75% precision, 77% recall and 76% F1 score. However, a certain part of the error is formed by non-uniform annotations of objects in the data because not all annotated foreign objects are located in the lung area, as stated in the description.
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Modeling of Respiratory System
Matoušek, Josef ; Roleček, Jiří (referee) ; Jiřík, Radovan (advisor)
This thesis is concerned with respiration system simulation. The main goal of this studies is to gain knowledge of how human respiration system works, along with selecting proper algorithms and their implementation into mathematic model. These models are realized by Simulink interface, which is part of Matlab environment. Similar models are used for medical technology research, especially for artificial ventilation of the lungs. There is a practical utilisation of these models suitable for illustration and respiration system's biological processes simulation, particularly combined model described in GUIDE graphic interface - also implemented in Matlab environment.
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Study of organs of mice in inhalation experiments
Vrlíková, Lucie ; Diviš, Pavel (referee) ; Dočekal, Bohumil (advisor)
The diploma thesis is devoted to study potential risks for living organisms resulting from inhalation of nanoparticles of chosen heavy metals. The main aim of this work is the investigation of organs from experimental white mice after their guided inhalation of lead nanoparticles. The samples of liver and lungs from these animals are taken in time periods during the inhalation experiments and processed by specific methods for their evaluation. After pressurized microwave assisted decomposition of selected samples of mice organs in high purity concentrated nitric acid the content of lead is determined by atomic absorption spectrometry with electrothermal atomization. Furthermore, the changes are monitored using the microscopy of histological slides stained with hematoxylin-eosin. The results of both approaches are evaluated and compared within the frame of this work.
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Influence of PbO and CdO nanoparticles on particular physiological functions in mouse.
Svozilová, Eva ; Vrlíková, Lucie (referee) ; Večeřa, Zbyněk (advisor)
The aim of this bachelor´s thesis is to assess the long-term effects of inhalation of nanoparticles of lead oxide and cadmium oxide on the weight of selected organs of experimental white mice. The selected organs (spleen, liver, kidney, lungs, brain) were successively collected during a period of thirteen weeks. The effect of inhalation of both metal oxides was statistically evaluated. In both study groups of the experiment (PbO and CdO), the relation between organs weight and the length of inhalation and the relation between organs weight and inhalation of differing metal concentrations were evaluated, and results both of the study groups were compared to each other.
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Transport and Deposition of Aerosol in Human Respiratory Tract
Elcner, Jakub ; Kratochvíl, Zdeněk (referee) ; Volavý, Jaroslav (referee) ; Jícha, Miroslav (advisor)
One of approaches in treatment of respiratory system diseases is the use of drug particles suspended in air in the form of aerosol. It is a fast and non-invasive method for the delivery of medicine into tracheobronchial tree or bloodstream. Although the method of the medication dosage by means of inhalers or nebulizers is well known, the effectiveness of that approach is still an actual issue. A significant amount of drugs delivered with the use of the medication dosage never reaches its primary destination and the drugs deposit in antecendent areas of respiratory tract where their presence is not required. This thesis deals with a problem of the passage of monodisperse homogenous aerosol with micron-size particles through the upper parts of the respiratory tract. This work was created with the use of numerical simulations carried out by means of the finite volume method in the commercial code based on computational fluid dynamics. Turbulence was modelled using the Reynolds averaged Navier–Stokes equations with the two-equation eddy viscosity k-omega SST model. The main output of the thesis is the analysis of airflow in two respiratory regimes. Stationary and cyclic cases of the flow behaviour were considered and the validation of simulated results with experiments performed on similar geometries was carried out. Furthermore, the review of simplified lung models and their geometries was made and the acquired results were used for the calculation of air distribution in the respiratory tract. The last part of the thesis deals with the calculation of particle deposition and with the analysis of the results.
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Ventilation Support of Laboratory Animals
Daniš, Václav ; Rozman, Jiří (referee) ; Sekora, Jiří (advisor)
mechanical ventilation an inseparable part of almost all surgery, where is anesthesia used. The introductory chapters of this thesis are focus on a teoretical familiarization with the complex issue with artificial lung ventilation. In additional to the history of artificial lung ventilation, chapters included familiarization with anatomy and physiology of lungs, associated with this defined volume of lungs and itself pulmonary ventilation. In the practical part I deal with design of ventilator for used it on laboratory animals.
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Air quality in buildings
Kops, Tomáš ; Uher, Pavel (referee) ; Rubinová, Olga (advisor)
The aim of the diploma thesis is to find out by measuring the production of carbon dioxide that man produces in various types of physical activity. Using the model, the goal is to simulate real carbon dioxide production for a type object and to design a way of automatic regulation, air supply and drainage to obtain better indoor environment (represented by carbon dioxide).
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Mobile Control Unit for Transporting Lungs
Sonnenschein, Jiří ; Veselý, Ivo (referee) ; Sekora, Jiří (advisor)
In the first part of this master’s thesis an overview about lungs (anatomy, physiology and respiratory mechanics) is given. Next sections are focused on description of the mechanical lung simulator and the mobile circulatory module. The use, construction, function, observed parameters and control scheme of the mobile circulatory module is described. Control unit which is divided into separate modules is introduced including description of power supply and communication. In following chapters a description of DCNM (Direct Control and Notification Module) is given.
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Data Transfer Protocol for Mobile Control Unit for Transporting Lungs
Trávníčková, Hana ; Mézl, Martin (referee) ; Sekora, Jiří (advisor)
The aim of this thesis is a data transfer protocol implementation for a mobile control unit for transporting lungs. Apart from this thesis the data transfer protocol is used in AlveoPic project. The introductory part is focused on an anatomical and physiological background of a human respiratory system. Consequently it describes the i-Lung module and the mobile circulation module (MCM). It deals with the healthcare informatics interoperability standards with an emphasis to the ISO/IEEE 11073 standard. The subsequent part is represented by MCM’s simulator realization and design of a monitoring application. The final part aims at an analysis of the test cases for a monitoring application’s and a protocol framework’s control.
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