|
|
Detection of single photon-upconversion nanoparticles by luminescence microcopy
Polachová, Natálie ; Kolář, Radim (referee) ; Fohlerová, Zdenka (advisor)
This bachelor thesis deals with the detection of photon-upconversion nanoparticles using the U-net convolutional neural network, by using epiluminescence microscopy. The theoretical part contains an introduction to the issue of photon-upconversion, description and use of photon-upconversion nanoparticles. Furthermore, the thesis deals with the functioning of basic and convolutional neural networks. In the practical part, we prepared samples of nanoparticles with subsequent acquisition of images by epiluminescence microscopy. The convolutional neural network U-net was designed, which further serves for the detection of nanoparticles bz using H-maxima morphological operations. In the end, everything was summarized and statistically evaluated..
|
|
|
Detection of single photon-upconversion nanoparticles by luminescence microcopy
Polachová, Natálie ; Kolář, Radim (referee) ; Fohlerová, Zdenka (advisor)
This bachelor thesis deals with the detection of photon-upconversion nanoparticles using the U-net convolutional neural network, by using epiluminescence microscopy. The theoretical part contains an introduction to the issue of photon-upconversion, description and use of photon-upconversion nanoparticles. Furthermore, the thesis deals with the functioning of basic and convolutional neural networks. In the practical part, we prepared samples of nanoparticles with subsequent acquisition of images by epiluminescence microscopy. The convolutional neural network U-net was designed, which further serves for the detection of nanoparticles bz using H-maxima morphological operations. In the end, everything was summarized and statistically evaluated..
|
|
|
Fast and highly sensitive laser scanner for recording photon-upconversion luminiscence from planar surfaces
Hlaváček, Antonín ; Křivánková, Jana ; Foret, František
Photon-upconversion nanoparticles (UCNPs) are lanthanide-doped nanocrystals that can be excited by nearinfrared light and emit photon-upconversion luminescence of shorter wavelengths. Advantages of UCNPs include near-infrared excitation, multiple and narrow emission bands, negligible autofluorescence and high stability, which make UCNPs ideal luminescence label for use in biological and chemical assays. These assays - e.g. upconversion-linked immunosorbent assay, western blot, lateral flow assay, gel electrophoresis, thin layer chromatography - commonly require the scanning of a planar surface with a high spatial resolution and an excellent sensitivity. The availability of commercial equipment is recently limited because of the novelty of the photon-upconversion phenomenon. Therefore, we report on the construction of photon-upconversion laser scanner. The scanner consists of a laser scanning head, which is attached to a xy-moving stage. The scanning head itself is constructed as an epiluminescence detector with excitation wavelength of 976 nm. A CCD array spectroscope is connected to the laser head and serves as a sensitive detector of photon-upconversion luminescence. The scanner possesses a spatial resolution of 200 μm, the scanning rate is up to 57 points per second and the sensitivity reaches down to single photon-upconversion nanoparticle.
|
|
| |
guest ::
