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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Application of tailor-made enzymes for biosyntheses of beta-lactam antibiotics
 Schneiderová, Michaela ; Kyslík, Pavel (advisor) ; Vopálenská, Irena (referee)
Nowadays beta-lactam antibiotics are widely used as bacteriostatic agents. The chemical synthesis of antibiotics or its derivatives could be replaced with biocatalysis. This work deals with basics of industrial synthesis beta-lactam antibiotics and, mainly, with used enzymes. This work acquainted with methodes used in enzyme modifications and improving, so they could fit the best for the industrial syntheses.
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Development of recombinant Pichia pastoris strain expressing synthetic gene of npga by genetic cloning
 Marešová, Helena ; Maršálek, Jaroslav ; Bečka, Stanislav ; Kyslík, Pavel
Cloning of synthetic npga gene into Pichia pastoris. Development of high expression system for penicillin acylase in a stirred bioreactor. Isolation and characterization of the enzyme.
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