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Comparison of physiological parameter data generated by the Metalyzer 3B in the laboratory and the Metamax 3B for use in field research
Kříž, Tomáš ; Nohel, Michal (referee) ; Chlíbková, Daniela (advisor)
Increasing quality of the sport performance creates demands related to sports data acquisition which occurs in the natural environment. Such demands are then placed on the equipment manufacturers which need to adapt and adjust equipment for artificial as well as the outdoor environment. Furthermore, required equipment must be substantially reduced in terms of its weight as well as the size to minimize possible interference with the outdoor conditions. The remaining question is to which extent such adjustments influence the validity of the data inquiry and whether laboratory testing should be prioritized before outdoor testing. This thesis aims to provide a comparison between two oxygen consumption analysers across the unified evidence-based testing protocol. The first half of the testing is made with Metalyzer 3B (laboratory-based equipment), and the second part of the testing is made with Metamax 3B (outdoor-oriented equipment). Both analysers are produced by the Cortex company. The theoretical part of this project introduces the underlying theory of the oxygen consumption analysers and presents various methods used for the establishment of the VO2max. The practical part includes the measurements of set parameters on both devices with an identical test protocol. The t-test was used to prove statistical significance between Metamax 3B and Metalyzer 3B. Results of statistical analysis (V'O2 p = 0,54; V'CO2 p = 0,087; SF p = 0,49; V'E p = 0,2; DF p = 0,28; time p = 0,59) within the range of 95% confidence intervals.
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Hemoglobin-mediated oxidation of marine liposomes
Škrabalová, Lada ; Mozuraityte, Revilija (referee) ; Rustad, Turid (advisor)
Cílem této práce bylo studium mechanismu oxidace lipidů katalyzované hovězím methemoglobinem a zhodnocení účinků různých experimentálních podmínek a antioxidantů (EDTA, askorbová kyselina, kávová kyselina, a-tokoferol, d-tokoferol, astaxanthin a L-askorbyl-6-palmitát) na methemoglobinem zprostředkovanou oxidaci lipidů v modelovém systému liposomů připravených z fosfolipidů. K monitorování oxidace lipidů při pH 5,5 a teplotě 30 °C bylo použito spotřeby kyslíku. Pro zhodnocení antioxidační aktivity v modelovém systému liposomů se ukázaly být důležitými faktory typ prooxidantu a koncentrace prooxidantu a antioxidantu. Dalšími důležitými faktory jsou struktura molekuly antioxidantu, jeho hydrofilita/lipofilita a umístění v systému. Všechny testované antioxidanty ve všech koncentracích (kromě koncentrace 0.1 % astaxanthinu and 0.1 % askorbyl palmitátu) inhibovaly oxidaci vyvolanou methemoglobinem. Účinnost antioxidantu stoupala s jeho zvyšující se koncentrací. Koncentrace 0.1 % astaxanthinu neměla žádný vliv na oxidaci liposomů. Koncentrace 0.1 % askorbyl palmitátu měla prooxidační efekt, který lze vysvětlit prooxidačním působením radikálu askorbylu, který může urychlit štěpení hydroperoxidů. Volné železo uvolněné z methemoglobinu se podílelo jen velmi málo na oxidaci liposomů, zatímco část prooxidační aktivity methemoglobinu byla přisouzena tvorbě singletového kyslíku (methemoglobin jako fotosenzitizátor). Antioxidační aktivita astaxanthinu, askorbyl palmitátu a tokoferolu byla z části přisouzena schopnosti zhášet singletový kyslík. Ovšem hlavním prooxidačním mechanismem methemoglobinu se ukázal být rozklad lipidových hydroperoxidů, tvorba volných radikálů a hypervalentních forem hemoglobinu. EDTA utlumila oxidaci liposomů díky chelataci přechodných kovů obsažených v liposomech a chelataci volného železa přítomného v methemoglobinovém roztoku. Velmi důležitým antioxidačním mechanismem (který vykazují askorbyl palmitát, askorbová a kávová kyselina) se ukázala být redukce hypervalentních forem hemoglobinu. Askorbová kyselina, kávová kyselina, tokoferoly a astaxanthin inhibovaly methemoglobinem zprostředkovanou oxidaci lipidů odstraňováním volných radikálů. Při použití peroxidu vodíku nebyl pozorován žádný vliv na oxidaci liposomů vyvolanou methemoglobinem. Působení vysoké teploty (tepelná denaturace) mírně utlumilo oxidaci. Významná inhibice oxidace byla pozorována u liposomů obsahujících TPP (triphenylphosphin), což značí, že je methemoglobinem vyvolaná oxidace liposomů závislá na přítomnosti již vzniklých lipidových peroxidů. Výsledky této práce přispívají k hlubšímu pochopení prooxidačních a antioxidačních mechanismů a faktorů, které ovlivňují oxidaci liposomálních roztoků, buněčných membrán a emulzí typu olej ve vodě stabilizovaných fosfolipidy.
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Comparison of physiological parameter data generated by the Metalyzer 3B in the laboratory and the Metamax 3B for use in field research
Kříž, Tomáš ; Nohel, Michal (referee) ; Chlíbková, Daniela (advisor)
Increasing quality of the sport performance creates demands related to sports data acquisition which occurs in the natural environment. Such demands are then placed on the equipment manufacturers which need to adapt and adjust equipment for artificial as well as the outdoor environment. Furthermore, required equipment must be substantially reduced in terms of its weight as well as the size to minimize possible interference with the outdoor conditions. The remaining question is to which extent such adjustments influence the validity of the data inquiry and whether laboratory testing should be prioritized before outdoor testing. This thesis aims to provide a comparison between two oxygen consumption analysers across the unified evidence-based testing protocol. The first half of the testing is made with Metalyzer 3B (laboratory-based equipment), and the second part of the testing is made with Metamax 3B (outdoor-oriented equipment). Both analysers are produced by the Cortex company. The theoretical part of this project introduces the underlying theory of the oxygen consumption analysers and presents various methods used for the establishment of the VO2max. The practical part includes the measurements of set parameters on both devices with an identical test protocol. The t-test was used to prove statistical significance between Metamax 3B and Metalyzer 3B. Results of statistical analysis (V'O2 p = 0,54; V'CO2 p = 0,087; SF p = 0,49; V'E p = 0,2; DF p = 0,28; time p = 0,59) within the range of 95% confidence intervals.
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Physiological responses on standardized climbing task in sport climbers
Gajdošík, Jan ; Baláš, Jiří (advisor) ; Radvanský, Jiří (referee)
Title: Physiological responses on standardized climbing task in sport climbers Purpose: To determine the effect of height, wall angle, climbing speed and climbing ability on physiological responses in sport climbers. Methods: The study was divided into three parts. 75 sport climbers (36 female and 39 male) completed differing tests on climbing wall and motorized climbing ergometer. Perceived exertion was assessed on a scale suggested by Borg. Indirect calorimetry, venous blood samples and near-infrared spectroscopy were used to assess physiological response, hormonal response and muscle oxygen saturation, respectively. Results: Perceived exertions were higher when climbing to height as opposes to climbing low to the ground on the treadwall (+5,3%; P = 0,013; ηp 2 = 0,149) (Study 1A). The physiological response was higher on the climbing wall as opposed to the treadwall: V̇ O2 (+6%; P = 0,03; ηp 2 = 0,22), SF (+4%; P = 0,04; ηp 2 = 0,20), V̇ E (+9%; P = 0,01; ηp 2 = 0,30) a EC (+16%; P < 0,001; ηp 2 = 0,48). There was an interaction for climbing ability and post- climbing catecholamine concentration (P < 0,01, ηp 2 = 0,28) (Study 1B). With increasing climbing speed greater differences were found for V̇ O2 (P < 0,001, ηp 2 = 0,923) than for StO2 (P < 0,001, ηp 2 = 0,448). Between-subject effect...
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Effect of climbing ability on energy expenditure of climbing
Kodejška, Jan ; Baláš, Jiří (advisor) ; Malý, Tomáš (referee)
The aim of the study was to evaluate the relation between climbing performance and energy demands while climbing through a specific submaximal test. The sample consisted of twenty-six climbers (age 26,8 ± 3,3 years, weight 70,6 ± 6,2 kg, height 177,6 ± 6,5 cm). Climbers were deliberately chosen so that their performance covers as much scale difficulty of UIAA (Union Internationale des Associations d'Alpinisme) as possible. The performance was given by the climbers currently the highest ascent in the style of RP (Red Point). Performance of these climbers ranged between 4 to 10 degrees UIAA. Climbers were subjected to submaximal climbing test, which lasted three minutes in one slope. They moved at a constant speed of 25 movements. min-1 on climbing route, already known in advance, the slope after 3 minutes changed from 90 ř to 105 ř. VO2 (oxygen consumption), SF (heart rate) and VE (minute ventilation) significantly correlated with climbing performance of RP (90ř, VO2, r = - 0,82; SF, r = -0,66, VE, r = - 0,77; 105ř, VO2, r = - 0,84; SF, r = -0,78; VE, r = - 0,80 ). Respiratory rate during submaximal climb was about 25 breaths. min-1, which refers to a link with a climbing speed. VO2 during submaximal climbing can be used to evaluate the economy of movement. Keywords sport climbing, submaximal test,...
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Determination of specific oxygen uptake during climbing test until exhaustion in female sport climbers
Kalábová, Monika ; Baláš, Jiří (advisor) ; Panáčková, Michaela (referee)
Title of bachelor thesis Determination of climbing specific oxygen uptake during climbing test to vita maxima of sports climbers. Aims To assess the determination of climbing specific oxygen uptake during climbing test to vita maxima of sports climbers. Methods The study involved 14 women climbers. Their climbing ability moved about since 3th to 10th degree of UIAA. Maximal oxygen uptake was measured on climbing wall, where climbers climbed the vertival wall profile (90ř) at their own pace for 3 minutes. Specific test started on 105ř profile with increased climbing speed until individual exhaustion every 3 minutes. After that, the participant went a maximal running test on treadmill. Results Climbers achieved average of specific oxygen uptake was 38,8 ± 6,6 ml·kg-1 ·min-1 in maximal climbing test in gradient 105ř and 51,5 ± 2,1 ml·kg-1 ·min-1 in maximal running test on treadmill. The climbing performance most corelated with achievement of speed r = 0,91 and oxygen uptake r = 0,75. Result values present that minimal level of specific VO2 max 45 - 50 ml·kg-1 ·min-1 is very important for climbing difficult ways. Conclusion Climbing test is suitable for controlled training status of sport climbers. Key words Sport climbing, physiology, climbing test, oxygen uptake
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Physiological responses on standardized climbing task in sport climbers
Gajdošík, Jan ; Baláš, Jiří (advisor) ; Radvanský, Jiří (referee) ; Vajda, Matej (referee)
Title: Physiological responses on standardized climbing task in sport climbers Purpose: To determine the effect of height, wall angle, climbing speed and climbing ability on physiological responses in sport climbers. Methods: The study was divided into three parts. 75 sport climbers (36 female and 39 male) completed differing tests on climbing wall and motorized climbing ergometer. Perceived exertion was assessed on a scale suggested by Borg. Indirect calorimetry, venous blood samples and near-infrared spectroscopy were used to assess physiological response, hormonal response and muscle oxygen saturation, respectively. Results: Perceived exertions were higher when climbing to height as opposes to climbing low to the ground on the treadwall (+5,3%; P = 0,013; ηp 2 = 0,149) (Study 1A). The physiological response was higher on the climbing wall as opposed to the treadwall: V̇ O2 (+6%; P = 0,03; ηp 2 = 0,22), SF (+4%; P = 0,04; ηp 2 = 0,20), V̇ E (+9%; P = 0,01; ηp 2 = 0,30) a EC (+16%; P < 0,001; ηp 2 = 0,48). There was an interaction for climbing ability and post- climbing catecholamine concentration (P < 0,01, ηp 2 = 0,28) (Study 1B). With increasing climbing speed greater differences were found for V̇ O2 (P < 0,001, ηp 2 = 0,923) than for StO2 (P < 0,001, ηp 2 = 0,448). Between-subject effect...
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Determination of climbing specific maximal oxygen uptake during climbing with increasing inclination and constant speed
Kaláb, Miloš ; Baláš, Jiří (advisor) ; Vomáčko, Ladislav (referee)
Title of master thesis Determination of climbing specific maximal oxygen uptake during climbing with increasing inclination and constant speed. Work objectives To review the determination of climbing specific maximal oxygen uptake during climbing with increasing inclination and constant speed. Methods Twenty six climbers with climbing ability on UIAA scale from 4th to 10th degree were participated in this study. Maximal specific oxygen uptake was measured by climbing test, to exhaustion on climbing wall, where the inclination were changed from 95ř (105ř) to 135ř every three minutes. Speed of climbing was constant on 25 movements·min-1 . After a rest, the participants went a maximal running test on treadmill. There were evaluated relationships between climbing ability, inclination and cardiopulmonary variables. Results In maximal climbing test, climbers achived plato on value 40 ± 3,5 ml·kg-1 ·min-1 , without influence on climbing ability or inclination. The climbing ability most corelated with achived inclination r = 0,89 and heart rate r = 0,41. Our study confirmed disproportional rising of heart rate against oxygen uptake. Furthermore, there was a strong relationship to be found between ventilation per 1l oxygen to RER indicating specific breathing mechanism of advanced climbers. More demanding...
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Determination of specific oxygen uptake during climbing test until exhaustion in female sport climbers
Kalábová, Monika ; Baláš, Jiří (advisor) ; Panáčková, Michaela (referee)
Title of bachelor thesis Determination of climbing specific oxygen uptake during climbing test to vita maxima of sports climbers. Aims To assess the determination of climbing specific oxygen uptake during climbing test to vita maxima of sports climbers. Methods The study involved 14 women climbers. Their climbing ability moved about since 3th to 10th degree of UIAA. Maximal oxygen uptake was measured on climbing wall, where climbers climbed the vertival wall profile (90ř) at their own pace for 3 minutes. Specific test started on 105ř profile with increased climbing speed until individual exhaustion every 3 minutes. After that, the participant went a maximal running test on treadmill. Results Climbers achieved average of specific oxygen uptake was 38,8 ± 6,6 ml·kg-1 ·min-1 in maximal climbing test in gradient 105ř and 51,5 ± 2,1 ml·kg-1 ·min-1 in maximal running test on treadmill. The climbing performance most corelated with achievement of speed r = 0,91 and oxygen uptake r = 0,75. Result values present that minimal level of specific VO2 max 45 - 50 ml·kg-1 ·min-1 is very important for climbing difficult ways. Conclusion Climbing test is suitable for controlled training status of sport climbers. Key words Sport climbing, physiology, climbing test, oxygen uptake
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