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The study of metallocene anticancer therapeutics-cells interaction
Bilavčíková, Kristýna ; Mravcová, Ludmila (referee) ; Vašinová Galiová, Michaela (advisor)
There were almost 600 thousand patients in Czech Republic until 2017 and the number keeps rising. Chemotherapeutics used today have lots of undesirable side effects, so scientists around the world are trying hard to find more specific and less cytotoxic drugs. In this bachelor’s thesis, potential drugs based on metallocene with a central atom of Ru, Hf or Zr were studied, always in three different drugs for each element. The permeability and distribution of these drugs into lung cancer cell lines were investigated. These drugs were detected by analysis of solutions and laser ablation with an ICP mass spectrometer. Based on the experiment, the greatest potential was found for the ruthenium-based drug 133, which had the highest permeability and was able to get into the cells in the highest volume.
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The study of metallocene anticancer therapeutics-cells interaction
Bilavčíková, Kristýna ; Mravcová, Ludmila (referee) ; Vašinová Galiová, Michaela (advisor)
There were almost 600 thousand patients in Czech Republic until 2017 and the number keeps rising. Chemotherapeutics used today have lots of undesirable side effects, so scientists around the world are trying hard to find more specific and less cytotoxic drugs. In this bachelor’s thesis, potential drugs based on metallocene with a central atom of Ru, Hf or Zr were studied, always in three different drugs for each element. The permeability and distribution of these drugs into lung cancer cell lines were investigated. These drugs were detected by analysis of solutions and laser ablation with an ICP mass spectrometer. Based on the experiment, the greatest potential was found for the ruthenium-based drug 133, which had the highest permeability and was able to get into the cells in the highest volume.
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Anticancer drugs in forms of nanoparticles and mechanisms potentiating their anticancer efficiency
Meskařová, Veronika ; Indra, Radek (advisor) ; Bělonožníková, Kateřina (referee)
Cancer has been one of the most common diseases of civilization for centuries. In the 18th century, some cancers were described and the first treatments were proposed. Currently, oncosurgery, chemotherapy, radiotherapy, immunotherapy and hormonal treatment are used to treat cancer. At the same time, efforts are being made to find new anticancer drugs that target tumor cells more selectively. Recently, nanomedicine has also started to be used. This bachelor thesis deals with minimizing the binding of the amount of cytostatic ellipticine to the surface of the nanotransporter apoferritin and achieving higher encapsulation efficiency. Two types of apoferritins at different weight ratios to ellipticine were studied. It has been found that by finding a suitable weight ratio of the two molecules, binding can be minimized, and encapsulation efficiency can be increased. When working with commercial apoferritin, there was a higher encapsulation and a lower binding of ellipticine to the surface at the weight ratio of 1:10. In contrast, when working with recombinant apoferritin, the encapsulation is higher and ellipticine binding are lower at the lower ratio of 1: 2,5. Key words: oncological diseases, anticancer drugs, nanomedicine, cytostatic, nanotransporter, ellipticine, apoferritin [IN CZECH]
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