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Native hyaluronan as a delivery system for hydrophobic drugs
Černá, Eva ; Mravec, Filip (referee) ; Pekař, Miloslav (advisor)
The aim of this paper is to discover whether it is possible to use the native form of hyaluronic acid as a hydrophobic drug carrier for a targeted distribution in the body. In its structure, hyaluronic acid is a linear high molecular weight biopolysaccharide which is found in most living organisms. Hyaluronan is involved in many physiological processes and therefore is essential for the functionality of the human body. It is in most tissues of the human body, high concentration is in the skin, the vitreous body and is also observed in cancer cells that contain several receptors for hyaluronan. These receptors include CD44 and RHAMM. The interaction of the hyaluronic acid delivery system and the hydrophobic medicinal with these receptors could ensure a free passage for drugs to the affected tissue, where the release of the drug would destroy the affected cells. The drug would directly target the damaged tissue and did not burden the rest of the body like the cytotoxic agents do. In this paper the native form of hyaluronic acid, which we normally find in the human organism, was chosen as the carrier. Its properties do not stand above other carrier systems, but its biocompatibility and biodegradability in the body greatly exceed them. High molecular weight hyaluronic acid was used as a carrier and the hydrophobic dye sudan red G, a substance of similar properties, was used instead of a hydrophobic drug.
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Native hyaluronan as a delivery system for hydrophobic drugs
Černá, Eva ; Mravec, Filip (referee) ; Pekař, Miloslav (advisor)
The aim of this paper is to discover whether it is possible to use the native form of hyaluronic acid as a hydrophobic drug carrier for a targeted distribution in the body. In its structure, hyaluronic acid is a linear high molecular weight biopolysaccharide which is found in most living organisms. Hyaluronan is involved in many physiological processes and therefore is essential for the functionality of the human body. It is in most tissues of the human body, high concentration is in the skin, the vitreous body and is also observed in cancer cells that contain several receptors for hyaluronan. These receptors include CD44 and RHAMM. The interaction of the hyaluronic acid delivery system and the hydrophobic medicinal with these receptors could ensure a free passage for drugs to the affected tissue, where the release of the drug would destroy the affected cells. The drug would directly target the damaged tissue and did not burden the rest of the body like the cytotoxic agents do. In this paper the native form of hyaluronic acid, which we normally find in the human organism, was chosen as the carrier. Its properties do not stand above other carrier systems, but its biocompatibility and biodegradability in the body greatly exceed them. High molecular weight hyaluronic acid was used as a carrier and the hydrophobic dye sudan red G, a substance of similar properties, was used instead of a hydrophobic drug.
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Introduction of the Histoscanning for Routine Evaluation of the Biopsy of the Native and Transplantated Kidneys.
ŠMOLÍKOVÁ, Petra
Aim: Immunofluorescence used as part of diagnostic algorithm for native and transplanted kidneys, however it has several limitations: short expiration, difficult handling and shipment. We compared quality of real slides versus scanned digital( virtual)slides. Materiál and methods: 163 cases were stained and diagnosed using classic technology and microscope. All cases were scanned using 3D Histech and evaluated again as virtual slides. Results: 20 primary evaluated slides( real and virtual) were reevaluated. Based on results, changes of technology were introduced ( medium, coverslips, scanning parameters). 143 new cases were examined using classic technology and subsequently using virtual microscopy. Results and minor differences in quality were acceptable and modified technology was introduced into the routine practice. Conclusions: Virtual microscopy is applicable also to immunofluorescence techniques and it is routinely used in our institution now. Altogether with histoscanning of standard histochemical and immunohistochemical stainings is also used for so-called telepathology. Main advantage is easy and fast access of consultant pathologist to examined materiál even from oversea distance.
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Vefication of Metadata for Sound Recordings
Přerovský, Petr ; Bartoň, Radek (referee) ; Láník, Aleš (advisor)
The work deals with verification of the metadata of audio recordings in an environment of mobile phones running Android. The text work is divided into three main parts. The first part is focused more on theoretical basis, where the main emphasis on the description of Android, application structure, description of the audio MP3 file and theoretical basis of Fourier transform in signal processing. The second part is devoted to solution design, analysis and implementation. The third part deals with testing.
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