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Optical Micromanipulation Techniques Combined with Microspectroscopic Methods
Pilát, Zdeněk ; Prášil,, Ondřej (referee) ; Mojzeš, Peter (referee) ; Zemánek, Pavel (advisor)
Předložená dizertační práce se zabývá kombinací optických mikromanipulací s mikrospektroskopickými metodami. Využili jsme laserovou pinzetu pro transport a třídění živých mikroorganismů, například jednobuněčných řas, či kvasinek. Ramanovskou spektroskopií jsme analyzovali chemické složení jednotlivých buněk a tyto informace jsme využili k automatické selekci buněk s vybranými vlastnostmi. Zkombinovali jsme pulsní amplitudově modulovanou fluorescenční mikrospektroskopii, optické mikromanipulace a jiné techniky ke zmapování stresové odpovědi opticky zachycených buněk při různých časech působení, vlnových délkách a intenzitách chytacího laseru. Vyrobili jsme různé typy mikrofluidních čipů a zkonstruovali jsme Ramanovu pinzetu pro třídění mikro-objektů, především živých buněk, v mikrofluidním prostředí.
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Recovery of waste from processing of phosphorus to produce materials with increased added value
Hejsek, Michal ; Zavřel, Tomáš (referee) ; Ing.Jan Červený, Ph.D. (advisor)
This master thesis focuses on reuse of waste water from industrial plant processing yellow phosphorus. Theoretical part summarizes physical and chemical properties of phosphorus, its transport in biosphere and its role in living organisms. Also the production process of the industrial plant is described. Waste water that contains waste byproducts is considered to be useful source of essential nutrients for economical large scale microalgae cultivation and development of biotechnological processes. In practical part, optimization of growth conditions for microalgae Chlorella pyrenoidosa Chick (IPPAS C-2) cultivation in medium based on wastewater from yellow phosphorus warehouse is presented.
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Microalgal Growth Problem for the Photosynthetitc Factory and its Optimization: An Overview and Further Analysis
Čelikovský, Sergej
The overview of the micro-algal growth problem for the photosynthetic factory and its optimal control solution is presented here. First, the model description and its properties like Haldan steady state dynamics, light integration property and the forward invariance of the set of biologically well-de ned states are described. This shows that the model meets basic experimentally based requirements. Further, its optimal control problem is formulated and solved analytically for the one dimensional singular perturbation based reduction. Properties of the optimal control for the reduced model are discussed and compared with some experimental observations. Among them, the general biotechnological paradigm that on large time intervals the optimal solution is, in certain sense, almost constant, is substantiated. As a new original result of this paper, the explicit solvable di erentiable equation for the unsaturated part of the optimal control is derived.
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Řasy jako surovina pro výrobu bioetanolu
Doucha, Jiří ; Lívanský, Karel ; Doušková, Irena ; Zachleder, Vilém
Many countries are actively developing biofuels program. Leading in this field is Brazil whose present need of gasoline is supplied with 30 % by ethanol produced relatively cheaply from sugarcane. In developed countries bioethanol (3-5 % of present gasoline need) is produced mostly from corn (USA) or wheat (EU). However, ethanol production from cereals is expensive. Promising feedstock are microalgae. Some of them can surpass yields of classical crop 10-15 times. Technology of growth of unicellular Chlorella in very thin layer on inclined areas is compared with the technology of world-wide mode of cultivation in open ponds. It was found, that due to higher yields and lower energy needs, the cost of biomass production in thin-layer bioractores is only 20 % compared with ponds
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