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The influence of physiologically controlled motor activity on biomechanical cross-section properties of long bones.
Jindrová, Alena ; Sládek, Vladimír (advisor) ; Špoutil, František (referee)
The mechanical loading affects the long bone cross-section geometry (CSG). The aim of this thesis was to examine experimentally the relationship between the locomotory parameters and cross-sectional properties of long bones, and to test some partial knowledge of the bone mechanical adaptation. Two groups of B6CBA mice were examined: (a) Lurcher type (model of olivocerebellar degeneration; n = 10) and (b) control (normal mice; n = 10). We analyzed the motor characteristics and the bone cross-sectional geometric properties. The motor tests included the test of spontaneous motor activity (Open field), strength properties (horizontal bar) and motor coordination (rotarod). Cross-sections were taken in 50 % of the left tibia biomechanical length and further processed for fluorescence confocal microscopy. We analyzed the biomechanical properties of cortical bone cross-sections (software ImageJ). There were nonsignificant differences in CSG parameters (TA, CA, Imax, Imin, J, Imax/Imin) between Lurcher and control mice. The results did not support our assumption about the effect of motor disorder on CSG properties. We did not demonstrate the effect of local factors on the bone biomechanical adaptation. The results of this thesis may be useful to find new testing possibilities of cortical bone in...
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Development of human postcranial skeleton depending on terrain
Turková, Eliška ; Sládek, Vladimír (advisor) ; Hora, Martin (referee)
A human bone is a dynamic structure that changes tissue arrangement according to inner and outer stimuli. The outer stimuli include among others the mechanical, environmental, and climatic ones. The impact of mechanical stimuli on the bones shows already with natural activities such as walking and running through change in the shape of a transverse bone cross section. The changes in the bone cross section can be observed on bone findings of ancient populations. The cross section shape of the long bones of lower extremities changes from the Palaeolithic to Neolithic population in such a way that the circulation of transverse cross-section increases. The explanation of this trend lies in the change of subsistence strategies from the Palaeolithic to the Neolithic era. The subsistence economy of the Palaeolithic population demanded more movement in comparison to the Neolithic population that lived a more sedentary lifestyle. This trend might be challenged by few bone findings of the Neolithic population from the mountain regions (e.g. Liguria, Italy) whose transverse bone cross section are more of a elliptic shape. Those excavations are mostly situated in the mountain regions with a demanding terrain. The outcomes presented in this study suggest that terrain is one of the factors that affect the...
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Evaluation of influence of mechanical loading on differentiation of stem cells into smooth muscle cells
Pražák, Šimon ; Filová, Elena (advisor) ; Maxová, Hana (referee)
Cultivation of cells in bioreactors with mechanical load simulates the physiological conditions to which cells in the body are exposed. This technology has been used to induce the differentiation of stem cells from adipose tissue towards the phenotype of vascular smooth muscle cells, which can further serve to form vascular replacements. At present, there is no established strategy for cultivating stem cells while being exposed to mechanical stress. The main aim of this work was therefore to optimize the cultivation strategy and determine the ideal load parameters. Differentiation was analyzed by immunofluorescence of specific smooth muscle cell markers, α-actin and h1-calponin, which were quantified by Western blot. Extracellular matrix production was also detected by immunofluorescence staining. The outcome of this work is the establishment of ideal conditions of cell culture in a bioreactor with mechanical load, during which they differentiate into smooth muscle cells. Three types of scaffolds were used for cultivation; plasma treated glass, fibrin-coated glass and decelularized pericardium. Preliminary results show that smooth muscle differentiation was succesfully induced in human and porcine adipose tissue stem cells. Cells were analyzed after 3 and 7 days of culture. Developing a stem cell...
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Development of human postcranial skeleton depending on terrain
Turková, Eliška ; Sládek, Vladimír (advisor) ; Hora, Martin (referee)
A human bone is a dynamic structure that changes tissue arrangement according to inner and outer stimuli. The outer stimuli include among others the mechanical, environmental, and climatic ones. The impact of mechanical stimuli on the bones shows already with natural activities such as walking and running through change in the shape of a transverse bone cross section. The changes in the bone cross section can be observed on bone findings of ancient populations. The cross section shape of the long bones of lower extremities changes from the Palaeolithic to Neolithic population in such a way that the circulation of transverse cross-section increases. The explanation of this trend lies in the change of subsistence strategies from the Palaeolithic to the Neolithic era. The subsistence economy of the Palaeolithic population demanded more movement in comparison to the Neolithic population that lived a more sedentary lifestyle. This trend might be challenged by few bone findings of the Neolithic population from the mountain regions (e.g. Liguria, Italy) whose transverse bone cross section are more of a elliptic shape. Those excavations are mostly situated in the mountain regions with a demanding terrain. The outcomes presented in this study suggest that terrain is one of the factors that affect the...
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The influence of physiologically controlled motor activity on biomechanical cross-section properties of long bones.
Jindrová, Alena ; Sládek, Vladimír (advisor) ; Špoutil, František (referee)
The mechanical loading affects the long bone cross-section geometry (CSG). The aim of this thesis was to examine experimentally the relationship between the locomotory parameters and cross-sectional properties of long bones, and to test some partial knowledge of the bone mechanical adaptation. Two groups of B6CBA mice were examined: (a) Lurcher type (model of olivocerebellar degeneration; n = 10) and (b) control (normal mice; n = 10). We analyzed the motor characteristics and the bone cross-sectional geometric properties. The motor tests included the test of spontaneous motor activity (Open field), strength properties (horizontal bar) and motor coordination (rotarod). Cross-sections were taken in 50 % of the left tibia biomechanical length and further processed for fluorescence confocal microscopy. We analyzed the biomechanical properties of cortical bone cross-sections (software ImageJ). There were nonsignificant differences in CSG parameters (TA, CA, Imax, Imin, J, Imax/Imin) between Lurcher and control mice. The results did not support our assumption about the effect of motor disorder on CSG properties. We did not demonstrate the effect of local factors on the bone biomechanical adaptation. The results of this thesis may be useful to find new testing possibilities of cortical bone in...
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