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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Raman tweezers for sorting of living cells
 Pilát, Zdeněk ; Ježek, Jan ; Kaňka, Jan ; Šerý, Mojmír ; Jákl, Petr ; Zemánek, Pavel
We have developed an instrument for automatized analysis and sorting of living cells of unicellular algae and other micro-objects based on laser tweezers and Raman spectroscopy. The system comprises the Raman tweezers setup, special microfluidic chip, and a specialized software allowing image recognition, spectral analysis, and automated sorting functions. The resulting instrument allows non-destructive analysis of chemical properties of living cells and their automatic separation for further examination or cultivation.
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Raman tweezers: principle and applications
 Bernatová, Silvie ; Samek, Ota ; Pilát, Zdeněk ; Ježek, Jan ; Kaňka, Jan ; Šiler, Martin ; Zemánek, Pavel
Raman tweezers combines the optical trap for non-contact micromanipulation of micro-objects with Raman spectroscopy for chemical analysis of the sample. This combination presents an efficient tool for concurrent spectroscopic analysis of chemical composition and micromanipulation allowing sorting or probing of mechanical properties of the sample. Raman tweezers were successfully used for example in characterization of biomolecules like DNA, for sorting of cells and investigation of the link between their chemical and mechanical properties.
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