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Mathematical analysis in mass spectrometry
Vlachová Hutová, Eliška ; Adam, Vojtěch (referee) ; Valla, Martin (advisor)
In this bachelor work, there is theoretically described the principle of mass spectometry and tandem mass spectometry, usage the method in practices and description of functionality of separate analysers. Next, there is also mentioned the description of mass spectrum and the algorithm to its analysis. Practical part of the work describes the creation of analytic tool to evaluate spectrum and following usage of this programming tool for the practical analysis of flavonoid compounds, concretely isoflavonoid.
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A complex study of biological effects of some soya products
Ručková, Michaela ; Němcová, Andrea (referee) ; Márová, Ivana (advisor)
Soybeans and products thereof are considered as a very contradictory legume across layman and expert opinions. Some see it almost as a “superfood” while others avoid it due to its biologically active content with an unclear effect on the organism. As a lot of research on soybeans was already performed the content varies considerably and it is not easy to grasp the issue correctly. The objective of the research part of the thesis is a structuralized study of already published scientific knowledge to clarify the current state of the art. Experimental part of the thesis focuses on characterisation of soy and its products in terms of active ingredients content and determination of antioxidants and antimicrobial effect. After that, the cytotoxic effect on human heterogeneous colorectal adenocarcinoma epithelial cell line and mouse melanoma cell line is determined by in vitro testing. All obtained information and results of experimental part could possibly serve as a starting point for further study of soy and soy products, both in the dissertation and in the submission of a grant project.
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A complex study of biological effects of some soya products
Ručková, Michaela ; Němcová, Andrea (referee) ; Márová, Ivana (advisor)
Soybeans and products thereof are considered as a very contradictory legume across layman and expert opinions. Some see it almost as a “superfood” while others avoid it due to its biologically active content with an unclear effect on the organism. As a lot of research on soybeans was already performed the content varies considerably and it is not easy to grasp the issue correctly. The objective of the research part of the thesis is a structuralized study of already published scientific knowledge to clarify the current state of the art. Experimental part of the thesis focuses on characterisation of soy and its products in terms of active ingredients content and determination of antioxidants and antimicrobial effect. After that, the cytotoxic effect on human heterogeneous colorectal adenocarcinoma epithelial cell line and mouse melanoma cell line is determined by in vitro testing. All obtained information and results of experimental part could possibly serve as a starting point for further study of soy and soy products, both in the dissertation and in the submission of a grant project.
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Biologicky aktivní látky v sóji
Sedláčková, Eliška
Submitted master thesis summarizes current knowledge about biologically active sub-stances contained in soybeans (Glycine max L. Merrill). Mainly deals with problematics of isoflavones, mechanism of origin and effects on human healht. Then the theoretical part summarizes mainly modern instrumental technics using for determination of isofla-vones. The experimantal part is concentrated on identification of soy isoflavones gathered from eight varieties (Brunensis, Bohemians, Naya, Moravians, Silesia, Toutatis, Korus, Kofu) of soybeans cultivated in Czech Republic. For detection of free, conjugated and total isoflavones was used high performance liquid chromatography with mass spectro-meter (LC-MS/MS)
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Components of soybean (\kur{Glycine max} (L.) Merr.) seeds and their effects on human health
LESÁKOVÁ, Klára
Farmers in CR started to grow soybean plants in late nineties. Since that days sowing area of soybean got much larger. There are many ways how to prepare soybean seeds for human consumption. It is a source of valuable proteins. It is also contained of a lot of biological active compounds. Those compounds have positive impact on human health. Effects of phytohormones - isoflavones are the most explored. Consumption of isoflavones decrease presence of cancer and other diseases in population
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Sója - funkční potravina
Zedníčková, Pavlína
This diploma thesis is about soybean as functional food. Functional food contain components with positive effect on human health. In soybean there are lot of these components and very valued are phytoestrogens. Phytoestrogens has estrogenic activity with positive effect in prevention of osteoporosis, breast and prostate cancer, cardiovascular disease and menopausal problems. In experimental part was measured level of isoflavones in different kinds of soy food. The method for separation was high performance liquid chromatography with mass spectrometry.
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Koncentrace vybraných fytoestrogenů v krmné dávce dojeného skotu a jejich distribuce do krve a mléka
Bařinová, Michaela
Diploma thesis on the theme Concentration of selected phytoestrogens in the diet of dairy cattle and their distribution in to the blood and milk deals about transmittance of phytoestrogens from real fed TMR in the South Moravian conventional breedings of dairy cows into the blood plasma of animals and to milk as a product intended for hu-man consumption. A review of literature is devoted to the occurrence of phytoestrogens in forage and feed, their metabolism and effects on animals and humans. The experimental part of the thesis is devoted to the identification and quantification of selected phytoestrogens in feed and their penetration into blood plasma and milk. Determination of phytoestrogens was performed by HPLC - MS analysis.
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Fytoestrogeny v potravinářství
Došková, Markéta
Phytoestroegens are biologically active compounds naturally occuring in plant materials. This group consists mainly of polyphenols characterized by structural similarities with female hormone 17-beta-estradiol. Plants produce phytoestrogens as their secundary metabolities, primarily due to the reaction as a response to various stress. Agents with estrogen effect contained in foodstuffs bring very positive impact on our health. On the other hand, some of the estrogen compounds may be harmful to the health depending on the concentration and the complex state of the consumer, as well as mycoestrogens, xenoestrogens and antiestrogens. Also for this reason is necessary to detect and quantify them in the sample of food. The major problem encountered in the studies of their effect is large amount of a variety of other substances in biological material, and according to their complicated structure was important to establish a reliable method, that was used also in the practical part of my experiment. It is an UPLC-MS/MS -- combination of Ultra High Performance Liquid Chromatography and Tandem Mass Spectrometry preceded by complex sample preparation which involves also SPE purification ("solid phase extraction").
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Mathematical analysis in mass spectrometry
Vlachová Hutová, Eliška ; Adam, Vojtěch (referee) ; Valla, Martin (advisor)
In this bachelor work, there is theoretically described the principle of mass spectometry and tandem mass spectometry, usage the method in practices and description of functionality of separate analysers. Next, there is also mentioned the description of mass spectrum and the algorithm to its analysis. Practical part of the work describes the creation of analytic tool to evaluate spectrum and following usage of this programming tool for the practical analysis of flavonoid compounds, concretely isoflavonoid.
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